Figure 14.

Open accessCopyright infoDownload PDFeLife Lens

Recognition of familiar food activates feeding via an endocrine serotonin signal in Caenorhabditis elegans

Figure 14.

Figure 14.
Download figureOpen in new tabDownload powerpointFigure 14. Taste and/or smell of novel bacteria override the stimulatory effect of familiar bacteria on feeding.

(A) Experimental design for the feeding assay. Coding is as in Figure 1A. Each condition is coded by three symbols. The first and the second letter above the bar represent training and test food in order. The third symbol, below the bar, represents the conditioned media that was mixed with the test food. LB, Hsup and Dsup represent LB broth and conditioned media filtered from cultures of HB101 and DA1878, respectively. (BC) Conditioned media from novel bacteria override the stimulatory effect of familiar bacteria on feeding. The average values of the feeding rates presented in (B) are 218.5 ± 5.0, 197.5 ± 7.3, 214.6 ± 3.9, 197.9 ± 5.4, 195.8 ± 4.7, 201.6 ± 5.2 in order. The average values of the feeding rates presented in (C) are 199.4 ± 5.6, 169.2 ± 6.3, 197.2 ± 4.7, 175.8 ± 3.8, 174.0 ± 5.1 and 172.8 ± 6.7 in order. Data shown as mean ± SEM, n.s., not significant (p≥0.05), *p<0.05, **p<0.01; one-way ANOVA, post hoc Tukey test. The number of animals tested (n ≥ 3 independent assays per each group) is shown on each bar.

DOI: http://dx.doi.org/10.7554/eLife.00329.016