Figure 16. | Recognition of familiar food activates feeding via an endocrine serotonin signal in Caenorhabditis elegans

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Recognition of familiar food activates feeding via an endocrine serotonin signal in Caenorhabditis elegans

Figure 16.

Figure 16.
Download figureOpen in new tabDownload powerpointFigure 16. The schematic of the experimental setup for calcium imaging.

The microfluidic chamber consisted of a T-shaped channel formed in PDMS bonded to a glass coverslip. The main branch of the channel was connected to an inlet and outlet, allowing LB or bacterial solutions to flow through. Switching from LB to the bacterial solutions was achieved via an upstream valve. A smaller branch orthogonal to the first one contained the worm. This tapered channel allowed immobilization of the animal while exposing only the tip of the nose to the flowing LB or bacterial solutions.

DOI: http://dx.doi.org/10.7554/eLife.00329.018