Focusing on calcium oscillations in relation to neuronal morphology both in vitro and in vivo, rationally designed GCaMP-X provides a simple solution to neuronal toxicity of conventional GCaMP in imaging applications involving prolonged and/or strong expression of calcium probes.
Biological ages have the potential to provide aging-related information beyond chronological age and can be predictive of mortality independently of both chronological age and different types of biological ages.
Two-photon Ca2+ imaging and computational modeling reveal major developmental trajectories of spontaneous activity in developing CA1 and identify important roles of network bi-stability and synaptic input characteristics for hippocampal burstiness before eye opening.
Electrophysiological and live-cell imaging analyses identify a presynaptic function of Ca2+ influx via transient receptor potential melastatin 7 (TRPM7) channels.
Genetics, in vivo imaging, and unbiased chemical biology screens reveal that Trpv6 functions as a cellular quiescence regulator and delineates a Trpv6-mediated Ca2+ signaling pathway maintaining the quiescent state.
CaV1.3 channels of the short isoform are coupled via their C-terminal domains in a Ca2+-CaM-dependent manner, which facilitates Ca2+ influx and increases the discharge of hippocampal neurons.
A unique toolbox of novel mouse lines expressing optical effectors and sensors in cardiovascular, smooth muscle, and additional lineages allows extensive in vivo and ex vivo analysis of complex biological systems.
Heterogeneity in cytoplasmic calcium concentration alongside B cell activation and differentiation is measured intravitally using an interdisciplinary imaging approach and novel numerical analysis.