A polarized Moody/protein kinase A pathway plays an unrecognized central role in controlling the enormous cell growth and blood–brain barrier integrity in a highly coordinated spatiotemporal manner in brain homeostasis.

Two modes of GPCR endocytosis by β-arrestin are differentially coupled to downstream signaling.

Spatial restriction of GPCR signaling at nascent synapses by adhesion complexes drives excitatory synapse formation and specificity in the hippocampus.

A GPCR activated by the same agonist engages different conformational biosensors at different subcellular locations, suggesting that location-based conformations drive spatial encoding of signaling.

Different dynorphins, which signal similarly from the cell surface, can differentially localize G protein-coupled receptors to specific subcellular compartments and cause divergent receptor fates and distinct spatiotemporal patterns of signaling.

Animal G-protein-coupled bistable rhodopsins can regulate Gq- and Gi-mediated signaling in a light-dependent manner in neurons and cardiomyocytes, making them useful for analyzing the roles of GPCR signaling in vivo.

Chemically distinct opioid ligands promote selective protein recruitment by opioid receptors in intact cells.

A combination of state-of-the-art biochemical, biophysical, and computational methods revealed that water molecules play a central role in signal propagation through G protein-coupled receptors.

Protein kinase A (PKA) phosphorylates Raptor and inhibits mammalian target of rapamycin complex 1 (mTORC1) activity.

Membrane receptor proteins move through primary cilia largely by passive diffusion rather than by active intraflagellar transport.