Structural studies reveal the mechanism by which a HECT E3 ubiquitin ligase carries out E2-to-E3-to-substrate ubiquitin transfer and prioritizes target lysines for ubiquitination.
Maria Azkanaz, Aida Rodríguez López ... Vincent van den Boom
Heat shock induces relocalization of epigenetic modifiers to the nucleolus, which acts as a dedicated protein quality control center that is indispensable for recovery of epigenetic regulators and epigenetic modifications.
The chaperones Hsp70 and Sis1 collaborate to repress the heat shock response and are both transcriptional targets of the heat shock response, yet only Hsp70 acts as a negative feedback regulator of the heat shock response.
Catherine G Triandafillou, Christopher D Katanski ... D Allan Drummond
When translation stops, cells require intracellular acidification to turn on the conserved heat shock response during stress, and stress-triggered acidification (common in eukaryotes) is adaptive, promoting cell and population fitness.
Katelyn E Sadler, Francie Moehring, Cheryl L Stucky
Purinergic keratinocyte-to-sensory neuron signaling is a ubiquitous amplification mechanism that is required for normal mechanical, cold, and heat sensation in vivo.
Centrosomal Aurora A (AIR-1), together with cortical actomyosin flows, induces polarization of ECT-2, the activator of RHO-1, during polarization and cytokinesis, in order to promote furrow formation.
Quantitative dissection of the roles of chaperone binding and phosphorylation in regulating heat shock factor 1 leads to a predictive model of the dynamics of the yeast heat shock response.
Nikolai Hentze, Laura Le Breton ... Matthias P Mayer
Heat-induced local unfolding allows Hsf1 to form trimers and bind to DNA, which depends on Hsf1 concentration and is promoted, not inhibited, by Hsp90.
To promote longevity under heat stress shares yeast aging factors with progeny through a down-regulation of the diffusion barrier in the membranes between the mitotic cells.