Nick Owens, Thaleia Papadopoulou ... Pablo Navarro
In contrast to other transcription factors, CTCF and Esrrb rapidly regain binding after replication and remain bound to their targets during mitosis, preserving local nucleosome organization throughout the cell cycle.
Selective APC/C-mediated proteolysis of cyclin B drives progression through the metaphase-anaphase transition whilst wide-spread waves of dephosphorylation co-ordinate the subsequent events of mitotic exit.
Preventing premature interactions between microtubules and protein-based structures called kinetochores ensures that chromosomes are segregated by meiosis rather than mitosis in reproductive cells.
Claudia Pellacani, Elisabetta Bucciarelli ... Maria Patrizia Somma
Extensive cytological and biochemical analyses show that the conserved Sf3A2 and Prp31 splicing factors bind microtubules and the Ndc80 complex, playing direct mitotic functions in both Drosophila and human mitosis.
Teemu P Miettinen, Joon Ho Kang ... Scott R Manalis
High-resolution single-cell mass accumulation and protein synthesis rate measurements are used to quantify the extent, dynamics and consequences of animal cell growth in mitosis and cytokinesis.
Mitosis is found to act as a novel trigger for epithelial cell cannibalism, revealing an important link between cell division and death, of relevance to cancer and chemotherapy.
Marco Novais-Cruz, Maria Alba Abad ... Cristina Ferrás
Direct live-cell imaging of human cells, combined with RNA-seq, qPCR and in vitro reconstitution essays, reveal that mitotic progression, arrest, exit or death is independent of de novo transcription.
A detailed analysis of protein abundance and phosphorylation changes across mitotic subphases and interphase in asynchronously growing human cells has been enabled by combining FACS with quantitative MS-based proteomics.
Nadine Vincenten, Lisa-Marie Kuhl ... Adèle L Marston
The meiotic DNA recombination landscape is locally influenced by the kinetochore to minimize potentially deleterious pericentromeric crossover recombination.