Brian T Emmer, Geoffrey G Hesketh ... David Ginsburg
A CRISPR-based genomic screen identifies the ER cargo receptor SURF4 as a key determinant in the secretion of PCSK9, a protein that regulates human cholesterol levels and cardiovascular disease risk.
Drosophila HNF4 directs a developmental switch at the onset of adulthood that suppresses diabetes by promoting mitochondrial function and supporting glucose-stimulated insulin secretion.
A novel multi-hormones secreting chromaffin-like cell type was identified using single-cell RNA sequencing, suggesting the molecular mechanism of ectopic ACTH and CRH pheochromocytoma associated with Cushing’s syndrome.
Niels JM Verstegen, Sabrina Pollastro ... S Marieke van Ham
Single-cell RNA sequencing provides a detailed understanding of the cues controlling in vitro differentiation of human B cells into antibody-secreting cells (ASCs), uncovering a novel pre-ASC population present ex vivo in lymphoid tissues that progress through a germinal center-like state.
Owain J Bryant, Paraminder Dhillon ... Gillian M Fraser
Proteins for bacterial flagella biogenesis contain two discrete export signals, recognised sequentially by the flagellar Type III Secretion System to facilitate initial docking and subsequent opening of the export gate.
Reconstitution of interleukin-1β secretion in non-macrophage cells implicates how this pro-inflammatory cytokine enters into the unconventional pathway of secretion through autophagy.
Meghan E Wynne, Oluwaseun Ogunbona ... Victor Faundez
Current models of Alzheimer's disease that put mitochondria as an endpoint of disease should be reconsidered because genetic defects affecting mitochondria by themselves can also regulate Alzheimer’s disease risk factor apolipoprotein E (APOE) expression and secretion.
Jeong Min Chung, Michael J Sheedlo ... Melanie D Ohi
The Helicobacter pylori Cag T4SS has unique structural features, including unexpected symmetry mismatch between sub-complexes, with implications for protein secretion.
Goblet cells secrete mucins—which are key components of mucus—in a process that is regulated by calcium ions, which enter the goblet cells via a mechanism involving a channel protein called TRPM5.
The essential role of focal adhesion kinase in locally regulating insulin secretion and calcium responses in pancreatic beta cells is identified through the use of the pancreatic slice technique.
