410 results found
    1. Chromosomes and Gene Expression

    Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases

    Yujia Cai, Rasmus O Bak, Jacob Giehm Mikkelsen
    Lentiviral protein transduction offers a new approach for administration of custom-designed nucleases, leading to high-efficacy targeted gene editing and reduced off-target activity after virus-directed delivery of programmable nuclease proteins.
    1. Stem Cells and Regenerative Medicine
    2. Chromosomes and Gene Expression

    Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors

    Yujia Cai, Anders Laustsen ... Jacob Giehm Mikkelsen
    Building on previous work (Cai et al., 2014), HIV-derived lentiviral vectors are harnessed to insert user-defined genes into a safe locus in the genome of human stem cells.
    1. Genetics and Genomics

    RNA-programmed genome editing in human cells

    Martin Jinek, Alexandra East ... Jennifer Doudna
    A bacterial protein can be programmed with short RNA molecules to stimulate genome editing at specific sites in human cells.
    1. Cell Biology
    2. Developmental Biology

    circZNF827 nucleates a transcription inhibitory complex to balance neuronal differentiation

    Anne Kruse Hollensen, Henriette Sylvain Thomsen ... Christian Kroun Damgaard
    A neuron-specific circular RNA keeps neuronal differentiation in check by lowering transcription rates from key neuronal marker genes.
    1. Neuroscience

    Loss of ZBTB20 impairs circadian output and leads to unimodal behavioral rhythms

    Zhipeng Qu, Hai Zhang ... Ying Xu
    The zinc finger protein ZBTB20 is a morning and evening activity gating factor in mice.
    1. Genetics and Genomics

    PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans

    Heesun Kim, Yue-He Ding ... Craig C Mello
    PIE-1 engages SUMO to preserve the embryonic germline fate and also to promote the assembly of a MEP-1/Mi-2/HDA-1 chromatin remodeling complex required for inherited Argonaute-mediated gene silencing in the adult hermaphrodite germline.
    1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    PTH regulates osteogenesis and suppresses adipogenesis through Zfp467 in a feed-forward, PTH1R-cyclic AMP-dependent manner

    Hanghang Liu, Akane Wada ... Clifford J Rosen
    Loss or PTH1R-mediated repression of Zfp467 results in a pathway that increases Pth1r transcription via NFκB1 and thus cellular responsiveness to PTH/PTHrP, ultimately leading to enhanced bone formation.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters

    Lisa Baumgartner, Dominik Handler ... Julius Brennecke
    Kipferl, a ZAD zinc finger protein from Drosophila, determines the chromatin binding specificity of the heterochromatin protein variant Rhino, defining piRNA clusters and preventing Rhino sequestration by selfish Satellite repeats.
    1. Genetics and Genomics

    Human genetic analyses of organelles highlight the nucleus in age-related trait heritability

    Rahul Gupta, Konrad J Karczewski ... Vamsi K Mootha
    Although cellular organelles show a functional deterioration in aging, genetic loci associated with common age-associated disease instead nominate nuclear transcription factors across several age-related diseases.
    1. Chromosomes and Gene Expression

    Gene Therapy: Delivering the second revolution in site-specific nucleases

    Perry B Hackett, Nikunj V Somia
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