July 2014

Research articles

    1. Developmental Biology and Stem Cells
    2. Genes and Chromosomes

    H3K27 modifications define segmental regulatory domains in the Drosophila bithorax complex

    Sarah K Bowman et al.
    Segment-specific boundaries of histone modifications reveal the regulatory logic of a classic developmental control region.
    1. Biochemistry
    2. Biophysics and Structural Biology

    Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters

    Zachary Lee Johnson et al.
    Studies of the substrate selectivity of concentrative nucleoside transporters provide proof of principle for structure-based improvement of drug delivery by these transporters.
    1. Neuroscience

    Integrated action of pheromone signals in promoting courtship behavior in male mice

    Sachiko Haga-Yamanaka et al.
    Identification of two classes of mouse vomeronasal receptors for female pheromone cues suggests distinct gating mechanisms in pheromone-triggered reproductive behaviors.
    1. Cell Biology

    Intact protein folding in the glutathione-depleted endoplasmic reticulum implicates alternative protein thiol reductants

    Satoshi Tsunoda et al.
    Adapting a cytosolic enzyme that breaks down glutathione to function in the lumen of the endoplasmic reticulum challenges the long-held view that reduced glutathione fuels disulfide rearrangements during protein folding.
    1. Developmental Biology and Stem Cells
    2. Neuroscience

    GSK-3 signaling in developing cortical neurons is essential for radial migration and dendritic orientation

    Meghan Morgan-Smith et al.
    The kinase GSK-3 regulates cortical neuronal migration and dendritic orientation by phosphorylating key cytoskeletal proteins.
    1. Biophysics and Structural Biology
    2. Immunology

    Serum amyloid A is a retinol binding protein that transports retinol during bacterial infection

    Mehabaw G Derebe et al.
    Serum amyloid A proteins bind retinol with nanomolar affinity, and do so by forming oligomers that create a hydrophobic pocket that shields retinol from the aqueous environment.
    1. Cell Biology
    2. Genes and Chromosomes

    Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast

    Noah Dephoure et al.
    Aneuploidy produces a protein expression signature characteristic of altered metabolism and redox homeostasis, and the loss of UBP6 ameliorates several aneuploidy-associated phenotypes.
    1. Biophysics and Structural Biology
    2. Cell Biology

    Flagellar synchronization through direct hydrodynamic interactions

    Douglas R Brumley et al.
    A combination of experiment and theory shows that pairs of eukaryotic flagella can achieve robust synchronization solely through the action of the fluid that surrounds them.
    1. Neuroscience

    Enhancement of encoding and retrieval functions through theta phase-specific manipulation of hippocampus

    Joshua H Siegle, Matthew A Wilson
    The ability of mice to encode new memories or retrieve existing ones can be selectively manipulated by using optogenetics to inhibit hippocampal activity at specific phases of the theta cycle.
    1. Biophysics and Structural Biology
    2. Cell Biology

    Bidirectional helical motility of cytoplasmic dynein around microtubules

    Sinan Can et al.
    Unlike other cytoskeletal motors that produce torque in a specific direction, cytoplasmic dynein generates torque in either direction, resulting in bidirectional helical motility along microtubules.
    1. Neuroscience

    Adult-born granule cells mature through two functionally distinct states

    János Brunner et al.
    3-10 weeks old adult-born granule cells provide two temporally overlapping but functionally distinct neuronal cell populations by being sensitive to distinct aspects of their inputs.
    1. Biochemistry
    2. Cell Biology

    Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals by calcineurin

    Sohum Mehta et al.
    The ability of calcium waves to exert different effects on calcineurin activity depending on subcellular location is mediated by the distribution of calmodulin.
    1. Biochemistry
    2. Cell Biology

    Redox signaling via the molecular chaperone BiP protects cells against endoplasmic reticulum-derived oxidative stress

    Jie Wang et al.
    Direct modification by endogenous peroxide of a conserved cysteine in the molecular chaperone BiP decouples its ATPase and peptide-binding activities, allowing for enhanced polypeptide holdase activity during oxidative stress.
    1. Biochemistry
    2. Cell Biology

    LARP7 suppresses P-TEFb activity to inhibit breast cancer progression and metastasis

    Xiaodan Ji et al.
    Transcription elongation by the elongation factor P-TEFb promotes the epithelial–mesenchymal transition and metastasis of breast cancer cells, implicating inhibition of this factor as a potential treatment for the late stages of this cancer.
    1. Biochemistry
    2. Biophysics and Structural Biology

    Quantitative analysis of mammalian GIRK2 channel regulation by G proteins, the signaling lipid PIP2 and Na+ in a reconstituted system

    Weiwei Wang et al.
    The G protein subunits Gβγ and the signaling lipid PIP2 are simultaneously needed to activate the potassium ion channel GIRK2 to control the voltage across a lipid bilayer, while sodium ions modulate these molecules' effects.
    1. Neuroscience

    Orbitofrontal neurons acquire responses to ‘valueless’ Pavlovian cues during unblocking

    Michael A McDannald et al.
    Orbitofrontal cortex neurons fire in response to cues with no value independent of what can be inferred through features of the predicted outcome.
    1. Genomics and Evolutionary Biology
    2. Neuroscience

    Lhx1 maintains synchrony among circadian oscillator neurons of the SCN

    Megumi Hatori et al.
    A transcription factor called Lhx1 coordinates the activity of the brain's master circadian clock, the suprachiasmatic nucleus, by controlling communication between its constituent cells.
    1. Biochemistry
    2. Cell Biology

    Large-scale filament formation inhibits the activity of CTP synthetase

    Rachael M Barry et al.
    The accumulation of its product, cytidine triphosphate, encourages the enzyme CTP synthetase (CtpS) to form large-scale polymers and inhibits the enzyme's activity.
    1. Biophysics and Structural Biology

    High temperature sensitivity is intrinsic to voltage-gated potassium channels

    Fan Yang, Jie Zheng
    Highly temperature-sensitive behavior of voltage-gated potassium channels provides a mechanistic model for how heat-activated TRP channels serve as temperature and pain sensors.
    1. Microbiology and Infectious Disease

    Insect endosymbiont proliferation is limited by lipid availability

    Jeremy K Herren et al.
    The endosymbiont Spiroplasma can only proliferate if it can acquire sufficient hemolymph lipids from its insect host.
    1. Developmental Biology and Stem Cells

    The Hippo effector Yorkie activates transcription by interacting with a histone methyltransferase complex through Ncoa6

    Yun Qing et al.
    Elucidating the molecular mechanism by which the Hippo signaling effector Yorkie (Yki) functions as a transcriptional coactivator in growth control reveals the importance of a histone-modifying enzyme for this process.
    1. Cell Biology

    The quantitative architecture of centromeric chromatin

    Dani L Bodor et al.
    The number of CENP-A molecules at human centromeres helps to explain how this structure is built and epigenetically inherited.
    1. Biophysics and Structural Biology

    Designed α-sheet peptides inhibit amyloid formation by targeting toxic oligomers

    Gene Hopping et al.
    Novel designed alpha-sheet peptides inhibit amyloidosis in two different systems and preferentially bind the toxic oligomer.
    1. Neuroscience

    Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice

    Xueying Wang et al.
    Laser-ablating neurons of a single genetic class reveals that Dbx1-derived interneurons comprise core respiratory rhythmogenic and premotor circuits and provides quantitative cellular parameters that govern network functionality
    1. Neuroscience

    Disparate substrates for head gaze following and face perception in the monkey superior temporal sulcus

    Karolina Marciniak et al.
    A distinct cortical region serves head gaze following, and is needed to establish joint attention with others and to ultimately develop a theory of others' mind.
    1. Neuroscience

    Nucleus reuniens of the thalamus contains head direction cells

    Maciej M Jankowski et al.
    Neurons that provide information about the direction of the head are present in nucleus reuniens and can potentially directly influence spatial processing in the hippocampus.
    1. Biophysics and Structural Biology
    2. Cell Biology

    A structural model of the active ribosome-bound membrane protein insertase YidC

    Stephan Wickles et al.
    A cryo-EM structure combined with bioinformatics provide a structural view on a conserved co-translational membrane protein biogenesis pathway.
    1. Biochemistry
    2. Human Biology and Medicine

    Quantitative determinants of aerobic glycolysis identify flux through the enzyme GAPDH as a limiting step

    Alexander A Shestov et al.
    A new computational model of the Warburg Effect reveals that the rate-limiting step of glycolysis is variable, identifies new control mechanisms, and could help to predict the responses to targeting glycolysis to treat cancer.
    1. Biophysics and Structural Biology
    2. Cell Biology

    The pseudo GTPase CENP-M drives human kinetochore assembly

    Federica Basilico et al.
    Centromeric protein M (CENP-M) is required for the stabilization of a quaternary complex that plays a crucial role in kinetochore organization and function.
    1. Cell Biology

    The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster

    Guiran Xiao et al.
    Drosophila ZIP13 (Slc39a13), a presumed zinc importer, is responsible for iron delivery to the secretory pathway.
    1. Cell Biology
    2. Immunology

    Induction of homologous recombination between sequence repeats by the activation induced cytidine deaminase (AID) protein

    Jean-Marie Buerstedde et al.
    Intra- and intergenic deletions by repeat recombination broaden the mutagenic potential of the activation induced cytidine deaminase (AID) protein.
    1. Neuroscience

    Loss of Cdc42 leads to defects in synaptic plasticity and remote memory recall

    Il Hwan Kim et al.
    Conditional knockout of the small GTPase Cdc42 in excitatory neurons of the mouse forebrain leads to impaired long-term synaptic plasticity and impaired retrieval of remote memory.
    1. Genomics and Evolutionary Biology
    2. Microbiology and Infectious Disease

    The inherent mutational tolerance and antigenic evolvability of influenza hemagglutinin

    Bargavi Thyagarajan, Jesse D Bloom
    Deep mutational scanning was used to comprehensively quantify the effects of mutations to influenza hemagglutinin and shows that the virus possesses a high inherent mutational tolerance at key antigenic sites.
    1. Developmental Biology and Stem Cells
    2. Genes and Chromosomes

    Core promoter factor TAF9B regulates neuronal gene expression

    Francisco J Herrera et al.
    An orphan TBP-associated factor, TAF9B, works in conjunction with the histone acetyl-transferase PCAF as transcriptional co-regulators of neuronal differentiation.
    1. Cell Biology
    2. Developmental Biology and Stem Cells

    Specific polar subpopulations of astral microtubules control spindle orientation and symmetric neural stem cell division

    Felipe Mora-Bermúdez et al.
    Mammalian neural stem cells specifically regulate a subset of astral microtubules to govern the subtle changes in spindle orientation that underlie symmetric vs asymmetric cell division during embryonic cortical neurogenesis.
    1. Genomics and Evolutionary Biology
    2. Plant Biology

    Natural epigenetic polymorphisms lead to intraspecific variation in Arabidopsis gene imprinting

    Daniela Pignatta et al.
    Individual scientists, scientific communities and scientific journals can do more to assess the publication of irreproducible results, to promote good science, and to increase the efficiency with which the scientific community self-corrects.