Susanne Roosing, Matan Hofree ... Joseph G Gleeson
A supervised learning approach on a high-content genome-wide siRNA screen has identified 591 likely candidates for ciliopathies and facilitated in the discovery of KIAA0586 mutations in individuals with Joubert syndrome.
Kacper B Rogala, Nicola J Dynes ... Ioannis Vakonakis
SAS-5 forms oligomers, through a trimeric coiled coil and novel dimeric domain, that are necessary for centriolar localisation of SAS-5 and for centriole duplication.
Alexandre Hyafil, Lorenzo Fontolan ... Anne-Lise Giraud
Computational modelling shows that coupled theta and gamma oscillations in the auditory cortex can decompose speech into its syllabic constituents, and organize the neural spiking at faster timescale into a decodable format.
Joshua P Neunuebel, Adam L Taylor ... SE Roian Egnor
A microphone array enables the vocal contribution of each socially interacting individual to be quantified, and reveals that vocalizations are exchanged between the sexes during mouse courtship.
Christian SM Helker, Annika Schuermann ... Wiebke Herzog
Elabela-Apelin receptor signaling is a novel pathway that regulates angioblast migration, instead of the proposed migration regulation through Vascular endothelial growth factor.
Anna Caballe, Dawn M Wenzel ... Juan Martin-Serrano
ULK3 is a kinase in the abscission checkpoint that temporarily inactivates ESCRT-III proteins via phosphorylation, which is required to delay cytokinesis in response to defects in mitosis.
Matthew A Cottee, Nadine Muschalik ... Susan M Lea
The structure of the Ana2 Central Coiled-Coil Domain provides insight into how centriolar cartwheel components may be recruited and assembled, and indicates that cartwheel assembly involves more than just SAS-6 oligomerisation.
A new family of sterol-specific lipid transfer proteins has been found that anchors in the endoplasmic reticulum; some of these proteins stretch across membrane contacts and mediate sterol traffic from the plasma membrane.
Virginia Guarani, Elizabeth M McNeill ... J Wade Harper
A comprehensive analysis of the human MICOS complex has identified a novel subunit called QIL1 that is required for cristae junction formation in human cells and Drosophila, through its role in the assembly of the MICOS complex.
Stimuli are encoded differently in the brain when perceived consciously and unconsciously; for conscious perception, the representations are stronger in certain brain regions and they display more complex dynamics.
Electron cryo-tomography reveals a huge conformational change in the secretin domain of the type IV pilus machinery that occurs when the channel opens for pilus extrusion.
Catherine M Sweeney-Reed, Tino Zaehle ... Alan Richardson-Klavehn
Building on previous work (Sweeney-Reed et al., 2014), it is shown that the timing of the theta rhythm of the right anterior thalamic nucleus predicts human memory formation.
A rationally designed small molecule ATP-mimetic activates IRE1 and PERK signaling in cells by inducing conformational changes that template the assembly of higher-order enzymatically active structures.
Tahnbee Kim, Florentina Soto, Daniel Kerschensteiner
The mouse visual system is able to detect small moving objects due to the activity of VG3-amacrine cells, an unusual type of modulatory cell that uses the transmitter glutamate to activate retinal output cells.
Cristian A Lasagna-Reeves, Maxime WC Rousseaux ... Huda Y Zoghbi
Studies in a mouse model of spinocerebellar ataxia type 1 show that a protein called capicua stabilizes toxic ataxin-1 oligomers offering a possible explanation for regional patterns of neurodegeneration.
Dietmar Schreiner, Jovan Simicevic ... Peter Scheiffele
A technique called selected reaction monitoring can quantitatively detect highly diversified protein isoforms and their association with specific neuronal structures and complexes.
Thomas Pohlmann, Sebastian Baumann ... Michael Feldbrügge
An endosomal component employs a novel PAM2-like motif to recruit a key RNA-binding protein, which explains how mRNAs and associated ribosomes are attached to endosomes during coupled transport.
Complex chromosomal rearrangements similar to those described in cancer and developmental syndromes occur in plants during postzygotic genome elimination, a centromeric-derived incompatibility.
Neurons in the fruit fly olfactory system respond most strongly to the sudden appearance of an odor, and to odors that are changing rapidly in strength, but are relatively insensitive to the absolute levels of an odor.
A detailed model of the T7 replisome with the precise positions of the helicase and polymerase at the replication fork explains how catalysis by the two enzymes is coordinated.
Balint Z Kacsoh, Julianna Bozler ... Giovanni Bosco
Reproductive behavior is conferred from experienced to naïve Drosophila as a behavioral social strategy allowing naïve groups to anticipate a threat from a predator.
Andrew R Osborn, Khaled H Almabruk ... Taifo Mahmud
Many vertebrates are able to synthesize a sunscreen compound de novo and the pathway involved can be used for heterologous production of the compound in yeast.
Sébastien Jeay, Swann Gaulis ... Diana Graus Porta
The discovery and preclinical validation of a novel gene signature predictive for response to HDM2-targeting drug NVP-CGM097 could significantly improve the clinical response rate to all p53-HDM2 inhibitors.
A comprehensive battery of behavioral, electrophysiological and functional MRI techniques has allowed the development of a data-driven conceptual model of the tinnitus-hyperacusis network.
Eugeni V Entchev, Dhaval S Patel ... QueeLim Ch'ng
TGFβ and serotonin signaling in a neural circuit encodes food availability; these signals regulate the dynamic range and variability on the circuit to impact coding accuracy and lifespan responses.
Small decreases in pH associated with cellular stress conditions unleash a cryptic mode of client binding in a ubiquitously expressed human small heat shock protein that is more effective at delaying client aggregation.
Gene knockdown experiments in fruit fly pupae reveal the role of the signaling molecule 'dFrizzled2' in the development of a neural circuit that helps insects to fly continuously for extended periods.
Two copies of a Polycomb target gene in the same cell can be maintained in independently heritable expression states, demonstrating that long-term memory of gene expression is stored locally.
Silvia Portugal, Christopher M Tipton ... Peter D Crompton
Atypical memory B cells (MBCs) appear to differentiate from classical MBCs during chronic exposure to the malaria parasite Plasmodium falciparum, and may interfere with the acquisition of immunity to the disease.
The secreted sugar-binding protein galectin-8 causes osteoblasts to secrete factors that promote the differentiation of bone marrow cells into osteoclasts; targeting this protein could therefore potentially help treat diseases associated with excessive bone loss.
Cytonemes mediate, and are essential for, Wingless signaling from the Drosophila wing disc to disc-associated myoblasts and for Delta-Notch signaling from these myoblasts to the dorsal air sac primordium.
Jae Yen Shin, Javier Lopez-Garrido ... Kit Pogliano
SpoIIIE forms a protein channel that spans the two lipid bilayers of the septum and mediates chromosome translocation and reversible membrane fission during Bacilus subtilis sporulation.
The Smc–ScpAB complex-a prokaryotic ancestor of cohesin, condensin and Smc5/6-loads onto the bacterial chromosome by employing ATP hydrolysis to capture DNA fibers within its tripartite ring.
The tarantula toxins psalmotoxin and guangxitoxin have a similar concave surface for interacting with α-helices in voltage-gated and acid-sensing ion channels.
Andrew N Gray, Alexander JF Egan ... Waldemar Vollmer
The protein CpoB regulates PBP1B activity in response to the Tol energy state, which facilitates feedback and synchronicity between envelope constriction processes during Gram-negative bacterial cell division.
Pierre Barbier de Reuille, Anne-Lise Routier-Kierzkowska ... Richard S Smith
MorphoGraphX summarizes full 3D datasets as curved surface images (2.5D), enabling the efficient quantification of growth and gene expression data from large time-lapse volumetric datasets.
Ruben Gepner, Mirna Mihovilovic Skanata ... Marc Gershow
Optogenetics and reverse-correlation have decoded the computations by which the Drosophila larva makes navigational decisions on the basis of time-varying sensory neuron activity.
Maximina H Yun, Hongorzul Davaapil, Jeremy P Brockes
Senescent cells are recurrently induced during limb regeneration in salamanders and subsequently eliminated by a highly efficient mechanism of macrophage-dependent surveillance.
Matthew T Kaufman, Mark M Churchland ... Krishna V Shenoy
Trial-by-trial analysis of neuronal activity in monkeys performing a decision-making task reveals the neural correlates of behaviours including wavering, hesitation and sudden changes of mind.
Luis Hernandez-Nunez, Jonas Belina ... Aravinthan DT Samuel
Optogenetic stimulation of odor- and taste-sensing neurons of the fruit fly larva combined with reverse-correlation analysis has been used to build mathematical models that predict navigational behavior.
Cellular hallmarks of thermal limits are evolutionarily conserved in nematodes, and changes in surface to volume ratio reflect restricted aerobic metabolism at these limits.
