Georges E Janssens, Anne C Meinema ... Matthias Heinemann
A comprehensive mapping of the proteome and transcriptome during the complete replicative lifespan of budding yeast predicted an increased abundance of the protein biogenesis machinery is most causal for aging.
Daniel A Keedy, Lillian R Kenner ... James S Fraser
The conformations of the enzyme cyclophilin A that are essential for its catalytic activity are temperature dependent and exhibit diverse responses, which is consistent with a complex energy landscape.
Chun-Chieh Lin, Katharine A Prokop-Prigge ... Christopher J Potter
The activation of Drosophila Or7a receptors guides aggregation and egg-laying behaviors towards both green leaf volatiles and a male-specific food-odor-induced pheromone.
The B-1a cell heavy chain antibody repertoire differs dramatically from the follicular and marginal zone B cell repertoire and is defined by distinct mechanisms driven by self antigens rather than antigens derived from the microbiota.
The secreted molecules FAM150A and FAM150B bind to the ALK RTK extracellular domain, potently activating the receptor activation and driving downstream signaling events.
The extrinsic cue NGF and the intrinsic signal Islet1 converge at the level of the Runx1/CBFβ transcription factor complex formation to promote differentiation of a major nociceptor subtype.
Large-scale electrocorticography and big data analysis of brain-wide neuronal interactions reveal the architecture of network information flow for context processing in primate brain.
Ryan M McCormack, Kirill Lyapichev ... George P Munson
Yersinia pseudotuberculosis and enteropathogenic Escherichia coli promote pathogenicity by deamidating the ubiquitin-like protein NEDD8 to block ubiquitin-dependent trafficking of Perforin-2, which is an effector of innate immunity.
Kevin C Hadley, Rishi Rakhit ... Avijit Chakrabartty
STOMP is a technique that can determine the proteomic composition of any feature that is identifiable by laser scanning microscopy and is at least one cubic micron in size.
Fernando J Santos, Rodrigo F Oliveira ... Rui M Costa
Recordings from the mouse brain as animals learn a lever pressing task reveal how the motor system optimizes skill learning by reducing variability in those aspects of task performance that are essential for achieving a goal.
In vivo recordings in unanesthetized zebrafish larvae show that Purkinje neurons have two stable membrane potential states and that climbing fiber inputs can toggle them to up states during motor episodes.
Oligomerization of p62/SQSTM1 generates high avidity binding regions that enable it to spatially select for concentrated ubiquitin and ATG8-family proteins.
Coarse-grained modeling reveals a new mechanism for multispanning membrane protein topogenesis, in which misintegrated configurations of the proteins undergo post-translational annealing to reach final, fully integrated multispanning topologies.
Philipp Khuc Trong, Hélène Doerflinger ... Raymond E Goldstein
A three-dimensional description of the cytoskeletal arrangement, cytoplasmic flows, and cargo transport in stage 9 Drosophila oocytes accurately reproduces mRNA localizations in wild-type and mutant oocytes.
Philip J Robinson, Michael J Trnka ... Roger D Kornberg
Integrative modeling of results from chemical cross-linking, electron microscopy, and homology modeling yield a three-dimensional model of the entire Mediator complex in yeast.
Discrimination of predictive and non-predictive fear stimuli requires plasticity in the lateral amygdala that is regulated by midbrain dopamine neurons.
A model based on the architecture of basal ganglia and validated with behavior and neuroimaging distinguishes mechanisms of action cancellation from no-go decisions.
The gene regulatory network controlling directed cell migration in a sea urchin is strikingly similar to a sub-circuit for eye development in Drosophila, suggesting that ancient systems-level controls may be adapted for diverse functions in different animals.
Evidence of an interaction between expected reward size and visual cortical microstimulation indicates that expected reward can affect sensory representations.
Diek W Wheeler, Charise M White ... Giorgio A Ascoli
Hippocampome.org is an online resource that provides free human- and machine-readable access to the comprehensive property-based classification of hippocampal neurons from 14,000 pieces of published experimental evidence.
Experimental and computational analyses reveal how proteasomal hydrolysis is regulated and show that peptide transport is the rate-limiting step and the main differentiating factor between human standard- and immuno-proteasomes.
The Hippo pathway downstream effector YAP regulates S-phase progression to protect neural stem cells of the retina from experiencing genomic instability.
Rebecca M Kilner, Giuseppe Boncoraglio ... Hanna Kokko
The adaptive value of social behaviour exhibited in adult life varies with conditions experienced in early life, and poorer conditions may promote conflict over cooperation.
Proteasome-mediated degradation of the TATA-box binding protein (TBP) during terminal differentiation is regulated by the E3 ligase Huwe1 and the deubiquitinase USP10.
Both within and between hosts, the key factor guiding whether increasing treatment strength will increase or decrease antibiotic resistance is whether inter-strain competition is effective, not whether it is present.
Susan H Taylor, Ching-Yan Chloé Yeung ... Karl E Kadler
The membrane type I-matrix metalloproteinase is essential for the release of collagen fibrils from plasma membrane fibripositors, which is required for the transition from embryonic to postnatal tendon development.
Anatomical, behavioral and physiological evidence suggests that the aPN1-vPN1-pC1 pathway serves as a labeled line for the processing and transformation of courtship song in male Drosophila.
Velicia Bachtiar, Jamie Near ... Charlotte J Stagg
Building on previous work (Stagg et al., 2014), it is shown that transcranial direct current stimulation modulates local GABA concentration and functional connectivity in the human motor cortex.
Chanpreet Singh, Grigorios Oikonomou, David A Prober
Experiments in genetically modified zebrafish that are unable to produce norepinephrine show that this neurotransmitter promotes wakefulness by mediating the effects of the neuropeptide hypocretin.
The C. elegans ninein homolog NOCA-1 is important for the assembly of non-centrosomal microtubule arrays required for embryo production, nuclear positioning and larval development.
Hung-Hsiang Huang, Antti Hassinen ... Pamela Stanley
GnT1IP-L is a membrane bound glycoprotein that interacts with MGAT1 in the Golgi, but not in the endoplasmic reticulum, to regulate complex N-glycan synthesis.
Shannon Burns, Jennifer S Avena ... Sue L Jaspersen
Visualization of yeast spindle pole proteins using a new microscopy method shows that assembly into the nuclear membrane occurs at the same time as duplication.
The well-established link between stress and depression could be due to the activity of a population of cells in prefrontal cortex that express a gene mutated in the rare disorder Wolfram syndrome.
The sigma subunit of bacterial RNA polymerase, classically known as an initiation factor, can also operate as an elongation factor with effects that vary with growth phase.
Correlated mutation analysis using a combination of amino acid and codon sequence alignments improves predictions of amino acid residue contacts in proteins.
Lizbeth Sayavedra, Manuel Kleiner ... Jillian M Petersen
Beneficial symbiotic bacteria encode an exceptional number of toxin-related genes that are all expressed by the symbionts in the host, supporting their key role in host-microbe interactions.
Finch embryos are laid at an earlier stage than other avian embryos and contain cells with similar properties to pluripotent embryonic stem cells from mice.
The small molecule NMDA-receptor antagonist MK801 has been genetically targeted to specific cell types in brain tissue to examine the role of NMDA receptors in cocaine-induced synaptic plasticity.
A new hominin species has been unearthed in the Dinaledi Chamber of the Rising Star cave system in the largest assemblage of a single species of hominins yet discovered in Africa.
Guy Wiedermann, Robert Alexander Bone ... J Kim Dale
Computational modelling together with experimental manipulation indicate that the stability and turnover of activated Notch is inextricably linked to the regulation of the pace of segmentation clock gene expression in the presomitic mesoderm.
Florian Fröhlich, Constance Petit ... Tobias C Walther
The retrograde vesicular trafficking GARP complex, which is mutated in a neurodegenerative disease, is important for sphingolipid homeostasis in yeast and mammalian cells.
The crystal structure of bacterial RNA polymerase bound to the transcription bubble reveals key features that support the formation of a double-strand/single-strand DNA junction at the upstream edge of the −10 element where bubble formation initiates.
As rats learn to traverse back and forth on a track, visual cortical neurons fire at specific locations and functionally interact with those hippocampal place cells representing the same locations.
The crystal structure of Thermus transcription activation complexes containing the transcriptional activator CarD reveals a new mechanism for the activation of transcription.
The activity patterns of populations of neurons in the retina are organized as semantic clusters (analogous to synonyms), in which component patterns bear little physical resemblance to one another but convey the same meaning.
Stefanie Hampel, Romain Franconville ... Andrew M Seeds
The identification of a neural circuit that drives a specific grooming movement in Drosophila reveals that it may also control movement parameters, such as duration.
Hybridization barriers in Arabidopsis thaliana can be established by paternally expressed imprinted genes (PEGs) that affect cellularization of the endosperm during seed development.
Severing axons in C. elegans reveals a mechanism by which the axons sense acute damage and respond by sequential remodeling of their microtubule cytoskeleton.
A new method of protein structure prediction that incorporates residue–residue co-evolution information into the Rosetta structure prediction program was used to develop models for 58 large protein families that had no previous structural information.
The cell junction-associated protein Amotl2a controls tissue size in the zebrafish lateral line primordium by limiting the activity of the Hippo effector Yap1 and the Wnt effector Lef1.
Xue-Xin Wei, Jason Prentice, Vijay Balasubramanian
Mathematical modeling suggests that grid cells in the rodent brain use fundamental principles of number theory to maximize the efficiency of spatial mapping, enabling animals to accurately encode their location with as few neurons as possible.
Kyle M Baumbauer, Jennifer J DeBerry ... Kathryn M Albers
Optogenetics reveals that keratinocytes can evoke action potential firing in several types of cutaneous sensory afferents, including those that transmit thermal, mechanical and pain stimuli.
Cell type-specific transcriptomics in energy homeostasis neurons has identified hundreds of genes and multiple signaling pathways that are regulated by weight loss in mice, as well as several previously unexamined genes that can regulate appetite and body weight.
Michael G Buhnerkempe, Katelyn Gostic ... James O Lloyd-Smith
Influenza transmission among ferrets correlates strongly with transmission among humans, so the ferret model for influenza transmission is a valid tool for pandemic potential in humans.
Endoplasmic reticulum (ER) inheritance regulation during ER stress controls the transmission of misfolded ER proteins from mother to daughter cell in budding yeast.
Diego Acosta-Alvear, Min Y Cho ... Martin Kampmann
The nodes of the cytosolic proteostasis network can control sensitivity to proteasome inhibitors and decreased levels of the 19S proteasomal regulator can lead to increased resistance to inhibitors.
Some species of bats hunt for insects that are resting on surfaces by detecting interruptions in the echoes from that surface, suggesting that resting on rough surfaces may help insects to evade detection by echolocation.
Peter Tsvetkov, Marc L Mendillo ... Susan Lindquist
Reducing the expression of 19S subunits shifts the ratio of 20S/26S proteasome complexes and provides a powerful survival advantage in the face of lethal proteasome inhibition.