A comprehensive mapping of the proteome and transcriptome during the complete replicative lifespan of budding yeast predicted an increased abundance of the protein biogenesis machinery is most causal for aging.
The B-1a cell heavy chain antibody repertoire differs dramatically from the follicular and marginal zone B cell repertoire and is defined by distinct mechanisms driven by self antigens rather than antigens derived from the microbiota.
The conformations of the enzyme cyclophilin A that are essential for its catalytic activity are temperature dependent and exhibit diverse responses, which is consistent with a complex energy landscape.
In vivo recordings in unanesthetized zebrafish larvae show that Purkinje neurons have two stable membrane potential states and that climbing fiber inputs can toggle them to up states during motor episodes.
Recordings from the mouse brain as animals learn a lever pressing task reveal how the motor system optimizes skill learning by reducing variability in those aspects of task performance that are essential for achieving a goal.
Yersinia pseudotuberculosis and enteropathogenic Escherichia coli promote pathogenicity by deamidating the ubiquitin-like protein NEDD8 to block ubiquitin-dependent trafficking of Perforin-2, which is an effector of innate immunity.
Coarse-grained modeling reveals a new mechanism for multispanning membrane protein topogenesis, in which misintegrated configurations of the proteins undergo post-translational annealing to reach final, fully integrated multispanning topologies.
A three-dimensional description of the cytoskeletal arrangement, cytoplasmic flows, and cargo transport in stage 9 Drosophila oocytes accurately reproduces mRNA localizations in wild-type and mutant oocytes.
Hippocampome.org is an online resource that provides free human- and machine-readable access to the comprehensive property-based classification of hippocampal neurons from 14,000 pieces of published experimental evidence.
The gene regulatory network controlling directed cell migration in a sea urchin is strikingly similar to a sub-circuit for eye development in Drosophila, suggesting that ancient systems-level controls may be adapted for diverse functions in different animals.
Experimental and computational analyses reveal how proteasomal hydrolysis is regulated and show that peptide transport is the rate-limiting step and the main differentiating factor between human standard- and immuno-proteasomes.
Both within and between hosts, the key factor guiding whether increasing treatment strength will increase or decrease antibiotic resistance is whether inter-strain competition is effective, not whether it is present.
The membrane type I-matrix metalloproteinase is essential for the release of collagen fibrils from plasma membrane fibripositors, which is required for the transition from embryonic to postnatal tendon development.
Computational modelling together with experimental manipulation indicate that the stability and turnover of activated Notch is inextricably linked to the regulation of the pace of segmentation clock gene expression in the presomitic mesoderm.
The crystal structure of bacterial RNA polymerase bound to the transcription bubble reveals key features that support the formation of a double-strand/single-strand DNA junction at the upstream edge of the −10 element where bubble formation initiates.
The activity patterns of populations of neurons in the retina are organized as semantic clusters (analogous to synonyms), in which component patterns bear little physical resemblance to one another but convey the same meaning.
Mathematical modeling suggests that grid cells in the rodent brain use fundamental principles of number theory to maximize the efficiency of spatial mapping, enabling animals to accurately encode their location with as few neurons as possible.
A new method of protein structure prediction that incorporates residue–residue co-evolution information into the Rosetta structure prediction program was used to develop models for 58 large protein families that had no previous structural information.
Cell type-specific transcriptomics in energy homeostasis neurons has identified hundreds of genes and multiple signaling pathways that are regulated by weight loss in mice, as well as several previously unexamined genes that can regulate appetite and body weight.