November 2022

Research articles

1. 1. Developmental Biology
   2. Neuroscience

   Presynaptic contact and activity opposingly regulate postsynaptic dendrite outgrowth

   Emily L Heckman, Chris Q Doe

   Opposing mechanisms determine dendrite arbor size during a critical period in Drosophila.
2. 1. Computational and Systems Biology
   2. Developmental Biology

   Deep proteome profiling reveals signatures of age and sex differences in paw skin and sciatic nerve of naïve mice

   Feng Xian, Julia Regina Sondermann ... Manuela Schmidt

   Proteomics uncovers thus far unknown age and sex differences in sciatic nerve and skin of naïve mice, highlighting the importance of adequate age matching and parallel investigation of male and female mice in biomedical studies.
3. 1. Cell Biology

   An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion

   Tricia T Nguyen, Gia K Voeltz

   ABHD16A is a phospholipid-modifying endoplasmic reticulum (ER) membrane protein required for both mitochondrial fission and fusion machinery recruitment to ER-associated mitochondrial constrictions.
4. 1. Developmental Biology
   2. Immunology and Inflammation

   Thymic macrophages consist of two populations with distinct localization and origin

   Tyng-An Zhou, Hsuan-Po Hsu ... Ivan L Dzhagalov

   Thymic macrophages are unique phagocytes with antigen-presenting abilities that comprise a Timd4⁺ subset of embryonic origin located in the cortex and a Cx3cr1⁺ subset derived from adult hematopoietic stem cells residing in the medulla.
5. 1. Biochemistry and Chemical Biology

   High-throughput proteomics of nanogram-scale samples with Zeno SWATH MS

   Ziyue Wang, Michael Mülleder ... Markus Ralser

   Zeno SWATH MS, a novel data-independent acquisition technique in proteomics, which by facilitating sensitive detection in high-throughput experiments with low sample amounts, mitigates current bottlenecks of high-throughput proteomics.
6. 1. Neuroscience

   Oscillations support short latency co-firing of neurons during human episodic memory formation

   Frédéric Roux, George Parish ... Simon Hanslmayr

   Synchronization of neural firing in the medial temporal lobe at fast, but not slow, theta and gamma oscillations correlates with shorter latencies of co-firing and successful memory formation.
7. 1. Evolutionary Biology

   Spinosaurus is not an aquatic dinosaur

   Paul C Sereno, Nathan Myhrvold ... Lauren L Conroy

   A digital flesh model of the sail-backed dinosaur Spinosaurus was tested and performed very poorly in water, favoring the view of this dinosaur as a two-legged, wading ambush predator of large fish in shallow waterways and not an aquatic dinosaur.
8. 1. Cell Biology
   2. Physics of Living Systems

   Fixation can change the appearance of phase separation in living cells

   Shawn Irgen-Gioro, Shawn Yoshida ... Shasha Chong

   Different fixation protocols can both enhance and diminish the appearance of liquid–liquid phase separation (LLPS) droplets presenting a caveat in using fixed-cell imaging to diagnose LLPS.
9. 1. Physics of Living Systems
   2. Plant Biology

   Environmental morphing enables informed dispersal of the dandelion diaspore

   Madeleine Seale, Oleksandr Zhdanov ... Naomi Nakayama

   The dandelion diaspore changes its shape depending on the moisture levels in the air, and this morphing enhances the dispersal potential of the seeds regardless of the weather conditions.
10. 1. Medicine
    2. Physics of Living Systems

    High spatial resolution analysis using automated indentation mapping differentiates biomechanical properties of normal vs. degenerated articular cartilage in mice

    Anand O Masson, Bryce Besler ... Roman J Krawetz

    The method presented here allows researchers to retrieve accurate and reproducible biomechanical properties of mouse cartilage.
11. 1. Ecology

    Tree species and genetic diversity increase productivity via functional diversity and trophic feedbacks

    Ting Tang, Naili Zhang ... Xiaojuan Liu

    Species and genetic diversity promote tree community productivity by increasing functional diversity and reducing herbivore damage and soil fungal diversity.
12. 1. Neuroscience

    Functional connectivity subtypes associate robustly with ASD diagnosis

    Sebastian GW Urchs, Angela Tam ... Pierre Bellec

    Unsupervised clustering of the heterogeneous functional brain organization in autism reveals generalizable patterns of connectivity alterations that extend to the general population.
13. 1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals

    Amrita A Iyer, Ishwar Hosamani ... Andrew K Groves

    Cells of the mammalian cochlea can be reprogrammed to produce mechanosensory hair cells, but epigenetic changes in the cochlea make this process less efficient with age.
14. 1. Ecology

    Latent functional diversity may accelerate microbial community responses to temperature fluctuations

    Thomas P Smith, Shorok Mombrikotb ... Thomas Bell

    A novel species-sorting experiment finds that phylogenetically and functionally distinct microbial communities emerge under different temperature conditions due to the resuscitation of latent diversity.
15. 1. Cell Biology

    A motor neuron disease-associated mutation produces non-glycosylated Seipin that induces ER stress and apoptosis by inactivating SERCA2b

    Shunsuke Saito, Tokiro Ishikawa ... Kazutoshi Mori

    When non-glycosylated, Seipin, which spans the endoplasmic reticulum (ER) membrane twice, forms aggregates in an oligomerization-dependent manner, distorts the shape of the ER, and incorporates SERCA2b via direct interaction, inactivation of which results in induction of ER stress and apoptosis.
16. 1. Neuroscience

    Improving the accuracy of single-trial fMRI response estimates using GLMsingle

    Jacob S Prince, Ian Charest ... Kendrick N Kay

    GLMsingle is a publicly available toolbox for analyzing fMRI time-series data that leverages data-driven optimization techniques to improve the accuracy of single-trial BOLD response estimates.
17. 1. Developmental Biology

    Conservation of oocyte development in germline cysts from Drosophila to mouse

    Allan C Spradling, Wanbao Niu ... Bhawana Maurya

    Early oocyte development takes place in germline cysts and is strikingly conserved from Drosophila to mouse, reflecting common mechanisms that may help reverse aging and specify oocyte and early embryo polarity.
18. 1. Developmental Biology
    2. Physics of Living Systems

    Mother cells control daughter cell proliferation in intestinal organoids to minimize proliferation fluctuations

    Guizela Huelsz-Prince, Rutger Nico Ulbe Kok ... Jeroen S van Zon

    Tracking of all cells in intestinal organoids crypts to fully reconstruct cell lineages reveals that proliferative behavior is highly symmetric between sisters cells, while simulations show that this observed symmetry minimizes random fluctuations in cell proliferation, thereby ensuring homeostasis.
19. 1. Computational and Systems Biology
    2. Developmental Biology

    Large-scale analysis and computer modeling reveal hidden regularities behind variability of cell division patterns in Arabidopsis thaliana embryogenesis

    Elise Laruelle, Katia Belcram ... Philippe Andrey

    Self-organization based on a feedback loop between cell geometry and division plane positioning plays a central role in the building of tissue architectures in Arabidopsis thaliana embryo through both stereotyped and variable division patterns.
20. 1. Neuroscience

    Coordinated multiplexing of information about separate objects in visual cortex

    Na Young Jun, Douglas A Ruff ... Jennifer M Groh

    Two distinct objects evoke fluctuating activity in visual cortex in a manner that could preserve information about both items.
21. 1. Microbiology and Infectious Disease

    Glutamine synthetase mRNA releases sRNA from its 3′UTR to regulate carbon/nitrogen metabolic balance in Enterobacteriaceae

    Masatoshi Miyakoshi, Teppei Morita ... Kan Tanaka

    Under nitrogen limitation, Salmonella and Escherichia coli express a messenger RNA to translate glutamine synthetase, and concomitantly the messenger RNA produces a small RNA from its 3′ end to inhibit the translation of 2-oxoglutarate dehydrogenase of the TCA cycle.
22. 1. Developmental Biology
    2. Genetics and Genomics

    Dysregulated H19/Igf2 expression disrupts cardiac-placental axis during development of Silver-Russell syndrome-like mouse models

    Suhee Chang, Diana Fulmer ... Marisa S Bartolomei

    Precise dosage of the imprinted H19 and Igf2 genes is critical for normal embryonic development, especially related to the cardiac-placental axis.
23. 1. Computational and Systems Biology
    2. Physics of Living Systems

    Metrics of high cofluctuation and entropy to describe control of cardiac function in the stellate ganglion

    Nil Z Gurel, Koustubh B Sudarshan ... Olujimi A Ajijola

    Networked cardiac control, revealed through analysis of extended baseline-state multi-channel recordings, differentiates heart failure and normal regimes highlighting the promise of peripheral neurally based clinical interventions.
24. 1. Immunology and Inflammation

    Cell-intrinsic ceramides determine T cell function during melanoma progression

    Matthias Hose, Anne Günther ... Wiebke Hansen

    Cell-intrinsic ceramide concentrations are involved in T cell differentiation and function.
25. 1. Neuroscience

    Dorsal striatum coding for the timely execution of action sequences

    Maria Cecilia Martinez, Camila Lidia Zold ... Mariano Andrés Belluscio

    In adolescent rats, whose actions are more impulsive, neuronal striatal activity that precedes self-initiated action sequences has a steeper modulation by waiting time compared to the modulation found in adults.
26. 1. Evolutionary Biology
    2. Genetics and Genomics

    Comparative genomics reveals insight into the evolutionary origin of massively scrambled genomes

    Yi Feng, Rafik Neme ... Laura F Landweber

    The comparison of three ciliate species that share complex pathways for natural genome editing allows capture of intermediate states in the acquisition of scrambled genes and elucidating a pathway for the origin and evolution of extremely rearranged chromosomes.
27. 1. Immunology and Inflammation

    EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

    Lyra O Randzavola, Paige M Mortimer ... David C Thomas

    Biochemical and cellular analyses reveal the mechanism by which EROS regulates NOX2 and P2X7 abundance in mouse and human.
28. 1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    N6-methyladenosine (m6A) reader Pho92 is recruited co-transcriptionally and couples translation to mRNA decay to promote meiotic fitness in yeast

    Radhika A Varier, Theodora Sideri ... Folkert J van Werven

    N6-methyladenosine (m6A) reader Pho92 is directed by Paf1C to meiotic mRNAs in an m6A-dependent and independent manner and promotes CCR4-NOT-mediated mRNA decay of m6A modified transcripts contingent on translation.
29. 1. Structural Biology and Molecular Biophysics

    Dilated cardiomyopathy mutation E525K in human beta-cardiac myosin stabilizes the interacting-heads motif and super-relaxed state of myosin

    David V Rasicci, Prince Tiwari ... Christopher M Yengo

    A dilated cardiomyopathy mutation can stabilize the auto-inhibited super-relaxed state and interacting-heads motif in human cardiac myosin, suggesting a mechanism by which disease mutations can reduce muscle force and power.
30. 1. Medicine

    Retinopathy of prematurity: Metabolic risk factors

    Zhongjie Fu, Anders K Nilsson ... Lois EH Smith

    An update for current knowledge gap in retinal metabolism after preterm birth and treatments for retinopathy of prematurity.
31. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Nucleotide-level linkage of transcriptional elongation and polyadenylation

    Joseph V Geisberg, Zarmik Moqtaderi ... Kevin Struhl

    Cleavage/polyadenylation, the process generating mRNA 3' ends, is linked at the nucleotide level to the position of RNA polymerase II, indicating a spatial coupling so polyadenylation occurs rapidly upon emergence of the nascent RNA from the Pol II elongation complex.
32. 1. Structural Biology and Molecular Biophysics

    Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein

    Kayla A Jara, Nikolaus M Loening ... Elisar J Barbar

    Autoinhibition by long-range intramolecular interactions in the partially disordered dynein intermediate chain is relieved by multivalent interactions of dynein light chains, demonstrating for the first time the dual roles of light chains in dynein assembly and regulation.
33. 1. Neuroscience

    Centrally expressed Cav3.2 T-type calcium channel is critical for the initiation and maintenance of neuropathic pain

    Sophie L Fayad, Guillaume Ourties ... Nathalie Leresche

    Specific deletion of Cav3.2 channels in anterior pretectal neurons reduced mechanical and cold allodynia, pointing to Cav3.2 channels as prime targets to develop innovative analgesic pharmacology that will not only act at the peripheral level but also in central structures.
34. 1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    The RAM signaling pathway links morphology, thermotolerance, and CO₂ tolerance in the global fungal pathogen Cryptococcus neoformans

    Benjamin J Chadwick, Tuyetnhu Pham ... Xiaorong Lin

    In the fungal pathogen Cryptococcus neoformans, the RAM pathway, potentially through post-transcriptional regulation, plays an essential role in adaptation to host temperature and CO₂.
35. 1. Neuroscience

    Cortex-wide response mode of VIP-expressing inhibitory neurons by reward and punishment

    Zoltán Szadai, Hyun-Jae Pi ... Balázs Rózsa

    VIP-expressing cortical interneurons represent organism-level information about reward and punishment for local microcircuits to regulate local processing and plasticity.
36. 1. Computational and Systems Biology
    2. Genetics and Genomics

    Transcriptome-wide association study and eQTL colocalization identify potentially causal genes responsible for human bone mineral density GWAS associations

    Basel Maher Al-Barghouthi, Will T Rosenow ... Charles R Farber

    Systems genetics approaches identify potentially causal bone mineral density genes.
37. 1. Physics of Living Systems

    Phenotyping single-cell motility in microfluidic confinement

    Samuel A Bentley, Hannah Laeverenz-Schlogelhofer ... Kirsty Y Wan

    A droplet microfluidics approach to stably trap and phenotype individual micron-sized algae reveals their distinct but stereotyped movement patterns and how they respond in real time to local environmental cues, including novel boundary circling behaviour under strong confinement.
38. 1. Ecology
    2. Microbiology and Infectious Disease

    Variation in the ACE2 receptor has limited utility for SARS-CoV-2 host prediction

    Nardus Mollentze, Deborah Keen ... Daniel G Streicker

    The success of angiotensin-converting enzyme 2 (ACE2)-based predictions of severe acute respiratory syndrome-associated virus 2 (SARS-CoV-2) host range stems from phylogenetic correlation, allowing development of scalable models which predict susceptibility to SARS-CoV-2 and other sarbecoviruses without requiring additional ACE2 sequencing.
39. 1. Cell Biology

    A general approach for stabilizing nanobodies for intracellular expression

    John G Dingus, Jonathan CY Tang ... Constance L Cepko

    A large proportion of nanobodies are unstable when expressed intracellularly, but can be reliably stabilized for intracellular expression by applying a generalizable mutagenesis strategy based on positional sequence conservation.
40. 1. Neuroscience

    Whole brain correlates of individual differences in skin conductance responses during discriminative fear conditioning to social cues

    Kevin Vinberg, Jörgen Rosén ... Fredrik Ahs

    A whole brain analysis using human neuroimaging data shows neural correlates of individual differences in conditioned fear.
41. 1. Medicine

    Exploratory data on the clinical efficacy of monoclonal antibodies against SARS-CoV-2 Omicron variant of concern

    Fulvia Mazzaferri, Massimo Mirandola ... Evelina Tacconelli

    Compared with casirivimab/imdevimab and bamlanivimab/etesevimab, early treatment with sotrovimab seems to reduce the time to sustained recovery among adult outpatients with mild-to-moderate SARS-CoV-2 infection due to Omicron BA.1 and BA.1.1.
42. 1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    Structure of human phagocyte NADPH oxidase in the resting state

    Rui Liu, Kangcheng Song ... Lei Chen

    The cryo-EM structure of a functional human NOX2-p22 complex in nanodisc in the resting state reveals the architecture of this key enzyme essential for innate immunity.
43. 1. Cancer Biology

    Global hypo-methylation in a proportion of glioblastoma enriched for an astrocytic signature is associated with increased invasion and altered immune landscape

    James Boot, Gabriel Rosser ... Silvia Marino

    A proportion of glioblastoma with global hypo-methylation are enriched for an astrocytic signature and display increased invasion and altered immune landscape.
44. 1. Epidemiology and Global Health
    2. Genetics and Genomics

    Age acquired skewed X chromosome inactivation is associated with adverse health outcomes in humans

    Amy L Roberts, Alessandro Morea ... Kerrin S Small

    Prospective, cross-sectional, and intra-twin study designs identify skewed X chromosome inactivation is associated with cardiovascular disease risk and future cancer diagnosis in the TwinsUK population cohort.
45. 1. Genetics and Genomics

    The genetic risk of gestational diabetes in South Asian women

    Amel Lamri, Jayneel Limbachia ... Sonia S Anand

    A type 2 diabetes polygenic risk score and family history of diabetes are strongly and independently associated with multiple gestational diabetes-related traits in women of South Asian descent.
46. 1. Ecology

    Bundling and segregation affect pheromone deposition, but not choice, in an ant

    Massimo De Agrò, Chiara Matschunas, Tomer J Czaczkes

    Ants are subjected to logarithmic value perception, causing deviation from pure rationality as described by classical economics decision theories.
47. 1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Molecular basis for the role of disulfide-linked αCTs in the activation of insulin-like growth factor 1 receptor and insulin receptor

    Jie Li, Jiayi Wu ... Eunhee Choi

    A critical role of aCT in the distinct activation mechanism of insulin receptor and insulin-like growth factor 1 receptor.
48. 1. Structural Biology and Molecular Biophysics

    Revealing druggable cryptic pockets in the Nsp1 of SARS-CoV-2 and other β-coronaviruses by simulations and crystallography

    Alberto Borsatto, Obaeda Akkad ... Francesco Luigi Gervasio

    A combination of simulations and experiments was used to discover druggable cryptic pockets in non-structural protein 1, a promising but difficult target for coronaviruses, indicating a viable drug discovery approach for otherwise undruggable targets.
49. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    m⁶A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5’ splice site

    Matthew T Parker, Beth K Soanes ... Gordon G Simpson

    High throughput sequencing of Arabidopsis mutants lacking the U6 snRNA methyltransferase FIONA1/METTL16 uncovers the role of U6 methylation in splicing signal preferences.
50. 1. Immunology and Inflammation

    SARS-CoV-2-specific CD4⁺ and CD8⁺ T cell responses can originate from cross-reactive CMV-specific T cells

    Cilia R Pothast, Romy C Dijkland ... Mirjam HM Heemskerk

    Insight into pre-existing SARS-CoV-2-specific T cell responses caused by heterologous T cell immunity directed against dissimilar epitopes, mediated by a public T cell receptor.
51. 1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Collateral deletion of the mitochondrial AAA+ ATPase ATAD1 sensitizes cancer cells to proteasome dysfunction

    Jacob M Winter, Heidi L Fresenius ... Jared Rutter

    A common genomic deletion in various human cancers disrupts mitochondrial protein homeostasis in a way that might be targeted by existing drugs.
52. 1. Developmental Biology
    2. Genetics and Genomics

    Genetic therapy in a mitochondrial disease model suggests a critical role for liver dysfunction in mortality

    Ankit Sabharwal, Mark D Wishman ... Stephen C Ekker

    A revertible Leigh Syndrome French Canadian Type (LSFC) disease model recapitulates the clinical phenotypes which can be rescued using a liver-specific genetic model therapy.
53. 1. Computational and Systems Biology
    2. Physics of Living Systems

    Measurements of damage and repair of binary health attributes in aging mice and humans reveal that robustness and resilience decrease with age, operate over broad timescales, and are affected differently by interventions

    Spencer Farrell, Alice E Kane ... Andrew D Rutenberg

    Damage and repair for both aging mice and humans declined with age, exhibited very long timescales that varied across health attributes, and were differently affected by interventions.
54. 1. Chromosomes and Gene Expression
    2. Immunology and Inflammation

    SRSF6 balances mitochondrial-driven innate immune outcomes through alternative splicing of BAX

    Allison R Wagner, Chi G Weindel ... Kristin L Patrick

    The splicing factor SRSF6 helps regulate antiviral immunity and mitochondrial membrane integrity by balancing the abundance of two alternatively spliced isoforms of the apoptotic protein BAX.
55. 1. Computational and Systems Biology
    2. Evolutionary Biology

    Evolution and regulation of microbial secondary metabolism

    Guillem Santamaria, Chen Liao ... Joao B Xavier

    Strains of the bacterium Pseudomonas aeruginosa that use secondary metabolism to make surfactants and change the surface tension of the liquid that surrounds them do so only if they can first meet their primary metabolic needs.
56. 1. Cell Biology
    2. Neuroscience

    Munc13 supports fusogenicity of non-docked vesicles at synapses with disrupted active zones

    Chao Tan, Giovanni de Nola ... Pascal S Kaeser

    Hextuple knockout of key active zone proteins strongly reduces the pool of synaptic vesicles available for release, but synapse formation persists.
57. 1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    Mettl3-mediated m⁶A modification of Fgf16 restricts cardiomyocyte proliferation during heart regeneration

    Fu-Qing Jiang, Kun Liu ... Xu-Feng Qi

    Mettl3 post-transcriptionally reduces Fgf16 mRNA levels through an m⁶A-Ythdf2-dependen pathway, thereby controlling cardiomyocyte proliferation and heart regeneration.
58. 1. Neuroscience

    Sugar sensation and mechanosensation in the egg-laying preference shift of Drosophila suzukii

    Wanyue Wang, Hany KM Dweck ... John R Carlson

    Drosophila suzukii, which lays eggs on ripe fruits, differs from Drosophila melanogaster in sweet sensation, mechanosensation, and their integration.
59. 1. Developmental Biology
    2. Genetics and Genomics

    Hedgehog regulation of epithelial cell state and morphogenesis in the larynx

    Janani Ramachandran, Weiqiang Zhou ... Steven A Vokes

    HH signaling prevents precocious EMT and cell death to maintain epithelial cell states during early stages of larynx development.
60. 1. Cell Biology
    2. Immunology and Inflammation

    A role for the centrosome in regulating the rate of neuronal efferocytosis by microglia in vivo

    Katrin Möller, Max Brambach ... Francesca Peri

    High-resolution imaging of microglia in zebrafish reveals a rate-limiting role for the centrosome in the engulfment of apoptotic neurons during brain development.
61. 1. Neuroscience

    Development of visual cortex in human neonates is selectively modified by postnatal experience

    Mingyang Li, Tingting Liu ... Dan Wu

    The structural and functional development of human infant visual cortex can be modulated by the external environment.
62. 1. Biochemistry and Chemical Biology

    Discovery of a new class of reversible TEA domain transcription factor inhibitors with a novel binding mode

    Lu Hu, Yang Sun ... Xu Wu

    Crystal structural analysis reveals a novel reversible pan-inhibitor of TEA domains with enhanced potency adopts a unique binding mode.
63. 1. Cell Biology
    2. Genetics and Genomics

    Unleashing a novel function of Endonuclease G in mitochondrial genome instability

    Sumedha Dahal, Humaira Siddiqua ... Sathees C Raghavan

    A new role for Endonuclease G with respect to the generation of mtDNA deletions is identified, which is dependent on the formation of G4 DNA within human mitochondria.
64. 1. Cell Biology

    Insulin sensitivity is preserved in mice made obese by feeding a high starch diet

    Amanda E Brandon, Lewin Small ... Gregory J Cooney

    Mice made obese by feeding a high starch diet do not develop insulin resistance like mice made similarly obese by a high fat diet and this is associated with differences in specific bioactive ceramide species in liver and muscle tissue.
65. 1. Structural Biology and Molecular Biophysics

    Three-dimensional flagella structures from animals’ closest unicellular relatives, the Choanoflagellates

    Justine M Pinskey, Adhya Lagisetty ... Daniela Nicastro

    Cryo-electron microscopy reveals previously undescribed structural features of choanoflagellate flagella and provides new insights into flagellar evolution.
66. 1. Neuroscience

    Phox2b mutation mediated by Atoh1 expression impaired respiratory rhythm and ventilatory responses to hypoxia and hypercapnia

    Caroline B Ferreira, Talita M Silva ... Thiago S Moreira

    New evidence for the impaired breathing activity is related to non-polyalanine repeat expansion mutations form of congenital central hypoventilation syndrome neuropathology.
67. 1. Neuroscience

    Phase separation of competing memories along the human hippocampal theta rhythm

    Casper Kerrén, Sander van Bree ... Maria Wimber

    The human brain uses a phase code to temporally segregate overlapping, competing memories along the phase cycle of the theta rhythm.
68. 1. Epidemiology and Global Health
    2. Immunology and Inflammation

    Quantifying the impact of immune history and variant on SARS-CoV-2 viral kinetics and infection rebound: A retrospective cohort study

    James A Hay, Stephen M Kissler ... Yonatan H Grad

    Variation in SARS-CoV-2 viral kinetics are partly explained by an individual's immune state and the infecting variant, but substantial interpersonal variation limits the reliability of isolation policies tailored to an individual's vaccination status.
69. 1. Neuroscience

    Binary and analog variation of synapses between cortical pyramidal neurons

    Sven Dorkenwald, Nicholas L Turner ... H Sebastian Seung

    Sizes of synapses between layer 2/3 pyramidal cells in mouse primary visual cortex are well modeled by the sum of a binary variable and an analog variable drawn from a log-normal distribution.
70. 1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Reevaluation of Piezo1 as a gut RNA sensor

    Alec R Nickolls, Gabrielle S O'Brien ... Alexander T Chesler

    Piezo1 ion channels are not modulated by RNA ligands, contrary to previous findings.
71. 1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for label-free detection of molecules across entire cell sections

    Johannes Elferich, Giulia Schiroli ... Nikolaus Grigorieff

    Cryo-electron microscopy can be used to detect biomolecules in entire 100- to 250-nm-thick cell slices when using a method to collect montages that prevents radiation damage in areas that have not been imaged yet.
72. 1. Cancer Biology

    The CIC-ERF co-deletion underlies fusion-independent activation of ETS family member, ETV1, to drive prostate cancer progression

    Nehal Gupta, Hanbing Song ... Ross A Okimoto

    Mutual suppression of ETV1 by Capicua (CIC) and ETS2 repressor factor (ERF) limits prostate cancer progression.
73. 1. Genetics and Genomics

    Genetic architecture of natural variation of cardiac performance from flies to humans

    Saswati Saha, Lionel Spinelli ... Laurent Perrin

    Comprehensive genome-wide associations studies in flies identify new genes and pathways critical for heart development and function and display relevant overlap with human genetic risks related to cardiac performance.
74. 1. Cell Biology
    2. Computational and Systems Biology

    Patterns of interdivision time correlations reveal hidden cell cycle factors

    Fern A Hughes, Alexis R Barr, Philipp Thomas

    Bayesian inference identifies hidden dynamics underlying noisy cell division data.
75. 1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    Promoter sequence and architecture determine expression variability and confer robustness to genetic variants

    Hjörleifur Einarsson, Marco Salvatore ... Robin Andersson

    A flexible transcription initiation architecture and a uniform regulatory grammar buffer expression variability and the effects of genetic variants on essential genes while a rigid transcription initiation architecture and a diverse regulatory grammar ensure condition-specific responsiveness of other genes.
76. 1. Genetics and Genomics
    2. Plant Biology

    Evolutionary gain and loss of a plant pattern-recognition receptor for HAMP recognition

    Simon Snoeck, Bradley W Abramson ... Adam D Steinbrenner

    A plant immune receptor evolved recognition of caterpillar peptides through key molecular changes.
77. 1. Neuroscience

    Cellular mechanisms underlying central sensitization in a mouse model of chronic muscle pain

    Yu-Ling Lin, Zhu-Sen Yang ... Cheng-Chang Lien

    Maladaptive changes of pro-nociceptive neurons in the central nucleus of the amygdala drive the chronification of muscle pain and comorbid affective behaviors.
78. 1. Epidemiology and Global Health
    2. Evolutionary Biology

    Taxonium, a web-based tool for exploring large phylogenetic trees

    Theo Sanderson

    A new tool allows researchers to dive into phylogenetic trees containing millions of nodes using just their web browser.
79. 1. Developmental Biology
    2. Evolutionary Biology

    A novel cis-regulatory element drives early expression of Nkx3.2 in the gnathostome primary jaw joint

    Jake Leyhr, Laura Waldmann ... Tatjana Haitina

    Jaw joint regulatory sequence 1 (JRS1) is deeply conserved in most jawed vertebrates and displays a specific enhancer activity in the developing primary jaw joint that contributes to early nkx3.2 gene expression and jaw joint morphology.
80. 1. Cell Biology

    Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome

    Kimberly Meechan, Wei Guan ... Yannick Schwab

    A new method using a motorised ultramicrotome and open-source software allows semi-automatic targeting of regions of interest for electron microscopy.
81. 1. Ecology
    2. Evolutionary Biology

    Contrasting parental roles shape sex differences in poison frog space use but not navigational performance

    Andrius Pašukonis, Shirley Jennifer Serrano-Rojas ... Lauren A O'Connell

    Extensive field studies in poison frogs reveal that sex and species differences in parental behavior drive differences in space use patterns but not navigational performance and highlight the interplay between androgen levels and poison frog spatial behavior.
82. 1. Ecology
    2. Evolutionary Biology

    Mandrill mothers associate with infants who look like their own offspring using phenotype matching

    Marie JE Charpentier, Clémence Poirotte ... Julien P Renoult

    Mandrill mothers know best because they use their offspring’s facial resemblance with other infants to guide their social opportunities towards similar-looking ones as an adaptive maternal behavior.
83. 1. Developmental Biology
    2. Neuroscience

    Channel-independent function of UNC-9/Innexin in spatial arrangement of GABAergic synapses in C. elegans

    Ardalan Hendi, Long-Gang Niu ... Kota Mizumoto

    Electrical synapses determine the precise position of chemical synapses in the nervous system.
84. 1. Cell Biology
    2. Neuroscience

    Endocytic trafficking determines cellular tolerance of presynaptic opioid signaling

    Damien Jullié, Camila Benitez ... Mark von Zastrow

    Quantitative imaging of neurons reveals how opioid agonists produce long-term functional tolerance at presynaptic terminals through rapid internalization of receptors from the axonal surface.
85. 1. Computational and Systems Biology
    2. Neuroscience

    Self-configuring feedback loops for sensorimotor control

    Sergio Oscar Verduzco-Flores, Erik De Schutter

    Learning to reach in the sensorimotor loop, and the required neural dynamics, can be potentially explained by simple principles.
86. 1. Microbiology and Infectious Disease
    2. Physics of Living Systems

    High-throughput imaging and quantitative analysis uncovers the nature of plasmid positioning by ParABS

    Robin Köhler, Eugen Kaganovitch, Seán M Murray

    The ParABS system positions plasmids precisely within the cell but just below the threshold for oscillatory dynamics.
87. 1. Genetics and Genomics

    Brown adipocytes local response to thyroid hormone is required for adaptive thermogenesis in adult male mice

    Yanis Zekri, Romain Guyot ... Karine Gauthier

    T3 regulates BAT thermogenesis by controlling the expression of genes involved in the management of metabolic fuels as well as tissue plasticity.
88. 1. Neuroscience

    Fast rule switching and slow rule updating in a perceptual categorization task

    Flora Bouchacourt, Sina Tafazoli ... Nathaniel D Daw

    Flexible cognition relies on a combination of fast inferential learning and slow incremental learning.
89. 1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers

    Martina Oravcová, Minghua Nie ... Michael N Boddy

    SIMC1 and SLF1 bind exclusively to SLF2 to form two separate complexes that direct the human SMC5/6 complex to its antiviral defense or DNA lesion repair activities.
90. 1. Microbiology and Infectious Disease
    2. Plant Biology

    Acetylation of a fungal effector that translocates host PR1 facilitates virulence

    Jingtao Li, Xiaoying Ma ... Wenxing Liang

    Acetylation stabilizes a fungal effector to mis-localize a key defense protein, leading to compromised immunity of host plants.
91. 1. Structural Biology and Molecular Biophysics

    Sublytic gasdermin-D pores captured in atomistic molecular simulations

    Stefan L Schaefer, Gerhard Hummer

    Molecular dynamics simulations reveal the formation of membrane pores by gasdermin-D, the ultimate effector of pyroptotic cell death in inflammation and infection.
92. 1. Structural Biology and Molecular Biophysics

    Structural and functional insights of the human peroxisomal ABC transporter ALDP

    Yutian Jia, Yanming Zhang ... Guanghui Yang

    The Cryo-EM structural and biochemical analysis provides a framework for understanding the working mechanism of ALDP.
93. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Molecular basis of the PIP₂-dependent regulation of Ca_V2.2 channel and its modulation by Ca_V β subunits

    Cheon-Gyu Park, Wookyung Yu, Byung-Chang Suh

    The anchoring properties of Ca_V β2 subunits to the plasma membrane determine the biophysical states of Ca_V2.2 channels by regulating PIP₂ coupling to the nonspecific phospholipid-binding site in the I–II loop.
94. 1. Structural Biology and Molecular Biophysics

    Structure of the GOLD-domain seven-transmembrane helix protein family member TMEM87A

    Christopher M Hoel, Lin Zhang, Stephen G Brohawn

    A cryo-EM structure of TMEM87A unexpectedly reveals it is structurally related to seven other proteins including Wntless, a chaperone for lipidated and secreted Wnt morphogens, suggesting GOLD-domain seven-transmembrane helix (GOST) proteins may all similarly traffic membrane-associated cargo.
95. 1. Neuroscience

    Nucleus accumbens dopamine tracks aversive stimulus duration and prediction but not value or prediction error

    Jessica N Goedhoop, Bastijn JG van den Boom ... Ingo Willuhn

    The extracellular concentration of dopamine in the nucleus accumbens core is progressively diminished by white noise, an underutilized, easy-to-titrate aversive stimulus, but is unaffected by white-noise intensity, context valence, and associated probabilistic contingencies.
96. 1. Ecology
    2. Genetics and Genomics

    Environmental DNA from archived leaves reveals widespread temporal turnover and biotic homogenization in forest arthropod communities

    Henrik Krehenwinkel, Sven Weber ... Michael Veith

    Environmental DNA from highly standardized leaf sample time series suggests no localized species losses, but biotic turnover and homogenization, as main drivers of forest insect decline.
97. 1. Genetics and Genomics

    An automated feeding system for the African killifish reveals the impact of diet on lifespan and allows scalable assessment of associative learning

    Andrew McKay, Emma K Costa ... Anne Brunet

    An automated feeding system to precisely control feeding in the killifish opens new areas to explore vertebrate lifespan, cognitive decline, dietary interventions, and drug screening in a high-throughput manner.
98. 1. Neuroscience

    Robotic multi-probe single-actuator inchworm neural microdrive

    Richard D Smith, Ilya Kolb ... Mladen Barbic

    A novel micropositioning approach allows remote-controlled, micrometer-resolution actuation of many densely packed, arbitrarily shaped probes with a device whose size and weight scale much more efficiently with probe count compared to current methods.
99. 1. Cell Biology

    Endosymbiotic selective pressure at the origin of eukaryotic cell biology

    Parth K Raval, Sriram G Garg, Sven B Gould

    All the characteristic traits of the eukaryotic cell, the basic unit of all macroscopic life, commenced to originate in a prokaryote as means to serve an endosymbiont today known as mitochondria.
100. 1. Chromosomes and Gene Expression

     Mcm2 promotes stem cell differentiation via its ability to bind H3-H4

     Xiaowei Xu, Xu Hua ... Zhiguo Zhang

     Mcm2 promotes mouse embryonic stem cells differentiation through manipulating chromatin landscapes at bivalent chromatin domains.
101. 1. Developmental Biology
     2. Stem Cells and Regenerative Medicine

     SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing

     Wenbo Liu, Xukun Lu ... Jianqiao Liu

     SRSF10 directly binds genes functioning in spermatogonia and regulates their alternative splicing, the defect of which can lead to progenitor expansion failure.
102. 1. Cell Biology

     Systematic analysis of membrane contact sites in Saccharomyces cerevisiae uncovers modulators of cellular lipid distribution

     Inês Gomes Castro, Shawn P Shortill ... Maya Schuldiner

     High-throughput analysis of several Saccharomyces cerevisiae contact sites reveals a large data set of potential contact site proteins, including proteins involved in the regulation of lipid distribution within cells.
103. 1. Computational and Systems Biology
     2. Physics of Living Systems

     Macroscopic control of cell electrophysiology through ion channel expression

     Mario García-Navarrete, Merisa Avdovic ... Krzysztof Wabnik

     A combination of mathematical modeling and live-cell imaging reveals a strategy for the rational control over cell electrophysiology though modulation of ion channel expression.
104. 1. Epidemiology and Global Health
     2. Neuroscience

     Attention-deficit hyperactivity disorder symptoms and brain morphology: Examining confounding bias

     Lorenza Dall'Aglio, Hannah H Kim ... Henning Tiemeier

     Approximately half of the associations identified between attention-deficit/hyperactivity disorder and brain morphology in two large independent studies of adolescents are likely due to confounding bias by socioeconomic and parental behavioral factors.
105. 1. Cell Biology

     Modeling single-cell phenotypes links yeast stress acclimation to transcriptional repression and pre-stress cellular states

     Andrew C Bergen, Rachel A Kocik ... Audrey P Gasch

     Counterintuitively, activation of the transcriptional repressor of growth-promoting genes is important for yeast cell acclimation to salt stress, such that cells with larger activation of the repressor tend to have faster growth acclimation after stress.
106. 1. Microbiology and Infectious Disease

     Phage resistance profiling identifies new genes required for biogenesis and modification of the corynebacterial cell envelope

     Amelia C McKitterick, Thomas G Bernhardt

     Using bacteriophages as probes, new genes involved in cell surface construction were identified in a class of bacteria that includes important pathogens like Mycobacterium tuberculosis.
107. 1. Neuroscience

     Learning-related contraction of gray matter in rodent sensorimotor cortex is associated with adaptive myelination

     Tomas Mediavilla, Özgün Özalay ... Daniel J Marcellino

     Learning produces dynamic white matter volumetric changes in the rodent brain observed by voxel-based morphometry on longitudinal structural MRI for which estimated white matter volume reflects adaptive myelination in the cortex.
108. 1. Computational and Systems Biology
     2. Immunology and Inflammation

     T cell receptor convergence is an indicator of antigen-specific T cell response in cancer immunotherapies

     Mingyao Pan, Bo Li

     Amino acid codon degeneracy indicates antigen-specific selection of the T cell receptors and is predictive of cancer immunotherapy outcomes.
109. 1. Microbiology and Infectious Disease

     Convergence of two global regulators to coordinate expression of essential virulence determinants of Mycobacterium tuberculosis

     Hina Khan, Partha Paul ... Dibyendu Sarkar

     The two global regulators CRP and PhoP interact with each other to coordinate expression of essential virulence determinants of Mycobacterium tuberculosis.
110. 1. Evolutionary Biology

     The molecular basis of socially induced egg-size plasticity in honey bees

     Bin Han, Qiaohong Wei ... Olav Rueppell

     Honey bee queens adjust the provisioning of their eggs based on their perception of colony size via upregulation of metabolism, protein transport, and cytoskeletal reorganization, including the small GTPase Rho1.
111. 1. Biochemistry and Chemical Biology
     2. Structural Biology and Molecular Biophysics

     Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex

     Caitlyn L McCafferty, Ophelia Papoulas ... Edward M Marcotte

     The 3D structure of the six-subunit complex and its polymeric assembly gives insights into cargo transport in cilia and how specific mutations in these genes lead to ciliopathy birth defects.
112. 1. Cell Biology

     LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation

     Chan Hee J Choi, William Barr ... Paul Cohen

     Profiling the adipocyte secretome reveals LRG1 as a novel adipokine that promotes insulin sensitivity and modulates inflammation triggered by release of cytochrome c from dead/dying cells, describing a new pathway at the intersection of obesity and its systemic sequelae.
113. 1. Neuroscience

     Spatiotemporal properties of glutamate input support direction selectivity in the dendrites of retinal starburst amacrine cells

     Prerna Srivastava, Geoff de Rosenroll ... Gautam Bhagwan Awatramani

     Two-photon glutamate imaging reveals that 'sustained' and 'transient' input are systematically arranged along single starburst dendrites, supporting the 'space-time' wiring model for direction selectivity.
114. 1. Cancer Biology

     Gene interaction perturbation network deciphers a high-resolution taxonomy in colorectal cancer

     Zaoqu Liu, Siyuan Weng ... Xinwei Han

     An individual-specific gene interaction perturbation network-based (GIN) approach reveals six stable GIN subtypes (GINS1-6) with distinguishing and high-resolution features.
115. 1. Microbiology and Infectious Disease

     An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteria

     Kuldeepkumar R Gupta, Celena M Gwin ... E Hesper Rego

     Asymmetric growth in mycobacteria requires a protein that is involved in trafficking mycolic acids to the outer membrane.
116. 1. Neuroscience

     Glutathione in the nucleus accumbens regulates motivation to exert reward-incentivized effort

     Ioannis Zalachoras, Eva Ramos-Fernández ... Carmen Sandi

     The capacity to sustain effort to obtain rewards over time depends on the levels of the antioxidant glutathione in the nucleus accumbens - a brain region critical for motivated behavior - and can be boosted with a nutritional intervention (N-acetylcysteine).
117. 1. Microbiology and Infectious Disease

     Targeted genomic sequencing with probe capture for discovery and surveillance of coronaviruses in bats

     Kevin S Kuchinski, Kara D Loos ... Andrew DS Cameron

     Hybridization probe capture is useful for discovery and surveillance of novel coronaviruses, and its unique strengths complement existing methods like amplicon sequencing and deep metagenomic sequencing.
118. 1. Neuroscience

     New insights into anatomical connectivity along the anterior–posterior axis of the human hippocampus using in vivo quantitative fibre tracking

     Marshall A Dalton, Arkiev D'Souza ... Fernando Calamante

     The results reported here provide new and foundational insights into the neural architecture that underpins hippocampal dependent memory systems in the human brain by providing detailed maps that show how different cortical areas anatomically connect within the hippocampus.
119. 1. Cancer Biology
     2. Genetics and Genomics

     Alternative splicing downstream of EMT enhances phenotypic plasticity and malignant behavior in colon cancer

     Tong Xu, Mathijs Verhagen ... Riccardo Fodde

     The identification of alternative splicing targets and upstream RNA-binding proteins that underlie EMT and metastasis in colon cancer stratifies patients according to their overall survival and provide new insights on the function of specific isoforms along the migrating cancer stem cell sequence.
120. 1. Genetics and Genomics
     2. Neuroscience

     Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III in Drosophila

     Justin A Bosch, Berrak Ugur ... Norbert Perrimon

     Sloth1 and Sloth2 are two mitochondrial peptides that are translated from one transcript and are important for neuronal function in Drosophila.
121. 1. Ecology
     2. Microbiology and Infectious Disease

     Gastrointestinal helminths increase Bordetella bronchiseptica shedding and host variation in supershedding

     Nhat TD Nguyen, Ashutosh K Pathak, Isabella M Cattadori

     Helminth infected hosts are an important cause of variation in the level, frequency and duration of Bordetella bronchiseptica shedding and dynamics of infection.
122. 1. Cell Biology

     Connexin 43 hemichannels regulate mitochondrial ATP generation, mobilization, and mitochondrial homeostasis against oxidative stress

     Jingruo Zhang, Manuel A Riquelme ... Jean X Jiang

     Mitochondrial Cx43 hemichannels regulate ATP generation by mediating K+, H+, and ATP transfer across the mitochondrial inner membrane and the interaction with mitochondrial ATP synthase, contributing to maintenance of mitochondrial redox and cell protection under oxidative stress.
123. 1. Cell Biology
     2. Physics of Living Systems

     Modeling and mechanical perturbations reveal how spatially regulated anchorage gives rise to spatially distinct mechanics across the mammalian spindle

     Pooja Suresh, Vahe Galstyan ... Sophie Dumont

     Coarse-grained modeling of mammalian kinetochore-fiber shapes generated under microneedle manipulation reveals the need for lateral anchorage 1-3 μm from the kinetochore to robustly preserve their orientation in the spindle center, promoting mechanical integrity in the dynamic spindle.
124. 1. Neuroscience

     Sampling motion trajectories during hippocampal theta sequences

     Balazs B Ujfalussy, Gergő Orbán

     The rodent brain represents uncertainty associated with short-term predictions during naturalistic navigation tasks sequentially by sampling hypothetical future trajectories in every ~100 ms, corresponding to successive theta cycles.
125. 1. Epidemiology and Global Health

     The role of adolescent lifestyle habits in biological aging: A prospective twin study

     Anna Kankaanpää, Asko Tolvanen ... Elina Sillanpää

     Unhealthy lifestyle habits in adolescence associate with accelerated biological aging in young adulthood, but shared genetic factors underlying both lifestyle and biological aging may largely explain the observed associations.
126. 1. Microbiology and Infectious Disease
     2. Neuroscience

     A prebiotic diet modulates microglial states and motor deficits in α-synuclein overexpressing mice

     Reem Abdel-Haq, Johannes CM Schlachetzki ... Sarkis K Mazmanian

     Microbiome-diet interactions shape microglia reactivity in a mouse model of Parkinson's disease.
127. 1. Evolutionary Biology
     2. Genetics and Genomics

     Complementary evolution of coding and noncoding sequence underlies mammalian hairlessness

     Amanda Kowalczyk, Maria Chikina, Nathan Clark

     Studying the genomes of mammals with sparse hair covering identifies specific genes and regulatory regions responsible for the formation of hair and skin, some of which were previously unrecognized.
128. 1. Biochemistry and Chemical Biology

     DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function

     Joshua Jeong, Joyce H Lee ... James M Berger

     The ability of eukaryotic topoisomerase IIs to undergo liquid-liquid phase separation is a novel process for this class of enzymes and may provide answers to many unexplained observations found in both in vitro and in vivo studies.
129. 1. Evolutionary Biology
     2. Neuroscience

     Whole-brain comparison of rodent and human brains using spatial transcriptomics

     Antoine Beauchamp, Yohan Yee ... Jason P Lerch

     A novel machine learning approach improves quantitative comparisons of mouse and human brains using spatial transcriptomics data sets.
130. 1. Neuroscience

     A connectomics-based taxonomy of mammals

     Laura E Suarez, Yossi Yovel ... Bratislav Misic

     Inter-species variations in connectome architecture across the mammalian phylogenetic spectrum recapitulate established taxonomic relationships defined by genetic, morphology, and behavioural differences, and are mainly driven by local changes in connectivity while hallmark global features tend to be conserved across species.
131. 1. Cell Biology

     Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia

     Pierre Mangeol, Dominique Massey-Harroche ... André Le Bivic

     Proteins pivotal to epithelial polarization organize in separated clusters, simplifying the current view by showing that a small fraction of the many protein–protein interactions proposed are prominent in mature epithelia.
132. 1. Microbiology and Infectious Disease

     T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus

     Matthew D Lauver, Ge Jin ... Aron E Lukacher

     Loss of T cell control during persistent polyomavirus infection, in the setting of a limited antiviral antibody response, facilitates emergence of antibody-escape viruses carrying the potential for neurovirulence.
133. 1. Genetics and Genomics

     Contrasting effects of Ksr2, an obesity gene, on trabecular bone volume and bone marrow adiposity

     Gustavo A Gomez, Charles H Rundle ... Subburaman Mohan

     Obese Ksr2 mutant mice have increased trabecular bone but decreased marrow adiposity and are more prone to fractures, thus providing a useful model to understand how stem cells can be manipulated to produce bone at the expense of marrow fat.
134. 1. Neuroscience

     Ultrafast simulation of large-scale neocortical microcircuitry with biophysically realistic neurons

     Viktor J Oláh, Nigel P Pedersen, Matthew JM Rowan

     Artificial neural networks can faithfully recapitulate realistic neuronal behavior with dramatically accelerated simulation runtimes, empowering accessible large-scale realistic network simulations.
135. 1. Neuroscience

     Sonic hedgehog-dependent recruitment of GABAergic interneurons into the developing visual thalamus

     Rachana Deven Somaiya, Katelyn Stebbins ... Michael A Fox

     Retinal ganglion cell axons release the morphogen sonic hedgehog which signals through astrocytes to induce the migration of inhibitory interneurons into the developing visual thalamus.
136. 1. Cell Biology

     High-resolution secretory timeline from vesicle formation at the Golgi to fusion at the plasma membrane in S. cerevisiae

     Robert M Gingras, Abigail M Sulpizio ... Anthony Bretscher

     The first quantitative live-cell microscopy this work shows depicting the order of events preceding secretion in budding yeast.
137. 1. Structural Biology and Molecular Biophysics

     Optimized tight binding between the S1 segment and KCNE3 is required for the constitutively open nature of the KCNQ1-KCNE3 channel complex

     Go Kasuya, Koichi Nakajo

     Guided by the available cryo-EM structure of the KCNQ1-KCNE3 ion channel complex, the critical role of the S1 segment of the voltage-sensor domain in the gating modulation by KCNE3 is revealed.
138. 1. Developmental Biology

     Translational rapid ultraviolet-excited sectioning tomography for whole-organ multicolor imaging with real-time molecular staining

     Wentao Yu, Lei Kang ... Terence TW Wong

     TRUST, as a rapid and fully automated whole-organ imaging technique, enables high-resolution multicolor 3D imaging at low cost by using a UV-LED for widefield illumination and a color camera while relieving researchers from lengthy and laborious tissue processing protocols.
139. 1. Computational and Systems Biology
     2. Neuroscience

     The interpretation of computational model parameters depends on the context

     Maria Katharina Eckstein, Sarah L Master ... Anne GE Collins

     Context factors such as experimental task and model parameterization can significantly impact modeling results, such that across tasks, the same participants show different fitted parameter values, and parameters capture different cognitive processes across tasks.
140. 1. Chromosomes and Gene Expression
     2. Genetics and Genomics

     Condensin DC loads and spreads from recruitment sites to create loop-anchored TADs in C. elegans

     Jun Kim, David S Jimenez ... Sevinc Ercan

     Loading and linear translocation of condensin dosage compensation along the X-chromosomes create the loop-anchored topologically associating domains detected by Hi-C.
141. 1. Stem Cells and Regenerative Medicine

     High-resolution imaging of the osteogenic and angiogenic interface at the site of murine cranial bone defect repair via multiphoton microscopy

     Kevin Schilling, Yuankun Zhai ... Xinping Zhang

     High-resolution multiphoton microscopy reveals coupling of blood vessel subtype with expanding osteoblasts at the bone regeneration interface, further highlighting the heterogeneity of oxygen microenvironment and its impact on cellular energy metabolism of bone and vessel forming cells.
142. 1. Developmental Biology

     A conserved function of Human DLC3 and Drosophila Cv-c in testis development

     Sol Sotillos, Isabel von der Decken ... James Castelli-Gair Hombría

     A gene causing human variants of sex development and its fly equivalent are required for testicular formation in both species.
143. 1. Computational and Systems Biology
     2. Neuroscience

     Mesoscale cortex-wide neural dynamics predict self-initiated actions in mice several seconds prior to movement

     Catalin Mitelut, Yongxu Zhang ... Timothy H Murphy

     Mouse self-initiated behaviors can be identified seconds prior to action and have similar neuro-dynamical structure to neural dynamics underlying human voluntary actions supporting the use of mouse models in self-initiated and volitional action research.
144. 1. Cancer Biology
     2. Medicine

     Non-coding RNAs in drug and radiation resistance of bone and soft-tissue sarcoma: a systematic review

     Huan-Huan Chen, Tie-Ning Zhang ... Tao Zhang

     Non-coding RNAs appear to be good candidates as biomarkers for predicting treatment response and therapeutics for sarcoma, their differential expression across tissues complicates their application.
145. 1. Neuroscience

     Intracranial human recordings reveal association between neural activity and perceived intensity for the pain of others in the insula

     Efe Soyman, Rune Bruls ... Valeria Gazzola

     Intracranial recordings indicate that the insula encodes, in a partially intermixed layout, both static and dynamic cues from different body parts that reflect the intensity of pain experienced by others.
146. 1. Chromosomes and Gene Expression
     2. Genetics and Genomics

     Genome-wide base editor screen identifies regulators of protein abundance in yeast

     Olga T Schubert, Joshua S Bloom ... Leonid Kruglyak

     A CRISPR/Cas9 base editor screen allows probing interactions between large numbers of known mutations and the abundance of specific proteins to study the protein regulatory network in yeast.
147. 1. Neuroscience

     Altered basal ganglia output during self-restraint

     Bon-Mi Gu, Joshua D Berke

     Rats are slower to initiate actions when these actions may need to be cancelled, and this slowing is associated with increased and more variable neural firing in the substantia nigra pars reticulata.
148. 1. Chromosomes and Gene Expression
     2. Structural Biology and Molecular Biophysics

     Mechanisms governing target search and binding dynamics of hypoxia-inducible factors

     Yu Chen, Claudia Cattoglio ... Xavier Darzacq

     Live-cell single particle tracking reveals the intrinsically disordered regions of transcription factors playing an unanticipated role in the nuclear search and chromatin binding process leading to functional target site selectivity and gene activation.
149. 1. Genetics and Genomics

     R-spondin 3 deletion induces Erk phosphorylation to enhance Wnt signaling and promote bone formation in the appendicular skeleton

     Kenichi Nagano, Kei Yamana ... Roland Baron

     R-spondin3 global haplo-insufficiency or its deletion in pre-osteoblasts lead to gain of bone mass in the appendicular skeleton with no or opposite phenotype in the axial skeleton.
150. 1. Cancer Biology
     2. Computational and Systems Biology

     Single-cell transcriptomics identifies Keap1-Nrf2 regulated collective invasion in a Drosophila tumor model

     Deeptiman Chatterjee, Caique Almeida Machado Costa ... Wu-Min Deng

     Comprehensive transcriptomic analyses describe the underlying tumorigenic gene expression state in the invasive Drosophila follicle cells following the loss of epithelial cell polarity and identify a non-canonical role of Keap1-Nrf2 signaling in regulating collective cell invasion.
151. 1. Neuroscience

     Microglia shape the embryonic development of mammalian respiratory networks

     Marie-Jeanne Cabirol, Laura Cardoit ... Muriel Thoby-Brisson

     Functional and anatomical analysis reveal a crucial role played during embryonic development by microglia in the ontogenesis and function of central circuits responsible for respiratory rhythmogenesis.
152. 1. Neuroscience

     Visual experience has opposing influences on the quality of stimulus representation in adult primary visual cortex

     Brian B Jeon, Thomas Fuchs ... Sandra J Kuhlman

     Stimulus representation in primary visual cortex rebounds to its original state following therapeutic sensory deprivation in adults.
153. 1. Neuroscience

     Functional interactions among neurons within single columns of macaque V1

     Ethan B Trepka, Shude Zhu ... Tirin Moore

     A proof-of-principle is provided for the use of high-density neurophysiological recordings in assessing local circuit interactions within the macaque brain.
154. 1. Neuroscience
     2. Physics of Living Systems

     Collective dynamics support group drumming, reduce variability, and stabilize tempo drift

     Dobromir Dotov, Lana Delasanta ... Laurel Trainor

     Human performance in a group synchronization task, described as a temporal version of the wisdom of crowds, can be understood as a phenomenon of the same nature as collective dynamics in swarming animals.
155. 1. Neuroscience

     Instructions and experiential learning have similar impacts on pain and pain-related brain responses but produce dissociations in value-based reversal learning

     Lauren Y Atlas, Troy C Dildine ... Daniel S Pine

     Combining computational models with FMRI in humans during pain reversal learning reveals parallel pain modulatory brain circuits that differ in their flexibility and sensitivity to instructions.
156. 1. Neuroscience

     Suppressed prefrontal neuronal firing variability and impaired social representation in IRSp53-mutant mice

     Woohyun Kim, Jae Jin Shin ... Eunjoon Kim

     Social deficits in IRSp53/Baiap2-mutant mice accompany decreases in prefrontal neuronal firing variability, burst firing, and social information decoding accuracy, and memantine-dependent NMDAR inhibition, which rescues social deficits, alleviates burst firing, suggesting that burst firing links NMDAR dysfunction with social deficits.
157. 1. Cell Biology
     2. Neuroscience

     Kiaa1024L/Minar2 is essential for hearing by regulating cholesterol distribution in hair bundles

     Ge Gao, Shuyu Guo ... Gang Peng

     Cholesterol plays an essential role in hair bundles, and Kiaa1024L/Minar2 regulates cholesterol distribution and homeostasis to ensure normal hearing.
158. 1. Cell Biology

     Regulated degradation of the inner nuclear membrane protein SUN2 maintains nuclear envelope architecture and function

     Logesvaran Krshnan, Wingyan Skyla Siu ... Pedro Carvalho

     Biochemical and genetic approaches revealed a posttranslational mechanism controlling the abundance of SUN2, a critical nuclear envelope protein.
159. 1. Neuroscience

     Rapid encoding of task regularities in the human hippocampus guides sensorimotor timing

     Ignacio Polti, Matthias Nau ... Christian F Doeller

     Hippocampal BOLD activity reflects behavioral performance and feedback in a fast-paced timing task, supporting the formation of task-specific yet flexible and generalizable sensorimotor representations in real time.
160. 1. Neuroscience

     Molecular and anatomical characterization of parabrachial neurons and their axonal projections

     Jordan L Pauli, Jane Y Chen ... Richard D Palmiter

     A guide summarizing the diversity of cell types in the parabrachial nucleus.
161. 1. Ecology

     Microplankton life histories revealed by holographic microscopy and deep learning

     Harshith Bachimanchi, Benjamin Midtvedt ... Giovanni Volpe

     The combination of holographic microscopy and deep learning provides a revolutionary tool for plankton ecology that will permit researchers to observe and study the life, feeding habits and reproduction of plankton with unprecedented detail.