April 2025

Research articles

1. 1. Cancer Biology

   Defined cellular reprogramming of androgen receptor-active prostate cancer to neuroendocrine prostate cancer

   Shan Li, Kai Song ... John K Lee
2. 1. Neuroscience

   Multiplexed CRISPRi Reveals a Transcriptional Switch Between KLF Activators and Repressors in the Maturing Neocortex

   Ryan W Kirk, Liwei Sun ... Sacha Nelson
3. 1. Neuroscience

   cxcl18b-defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina

   Aojun Ye, Shuguang Yu ... Chang Chen
4. 1. Plant Biology

   Targeted and random mutagenesis of cassava brown streak disease susceptibility factors reveal molecular determinants of disease severity

   ZJ Daniel Lin, Myia K Stanton ... Rebecca S Bart
5. 1. Biochemistry and Chemical Biology
   2. Neuroscience

   Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during microglial activation

   Benoit Arnould, Adeline Martz ... Thomas Grutter
6. 1. Structural Biology and Molecular Biophysics

   Engineering cardiolipin binding to an artificial membrane protein reveals determinants for lipid-mediated stabilization

   Mia L Abramsson, Robin A Corey ... Michael Landreh

   Bulding a cardiolipin binding site into a computationally designed transmembrane protein uncovers how proteins can recognize specific lipids and use them to regulate their function.
7. 1. Cell Biology
   2. Genetics and Genomics

   DNA replication in primary hepatocytes without the six-subunit ORC

   Róża K Przanowska, Yuechuan Chen ... Anindya Dutta

   Mammalian cells are capable of loading MCM2-7 to support DNA replication in the absence of ORC to permit extensive DNA replication in vivo.
8. 1. Computational and Systems Biology
   2. Microbiology and Infectious Disease

   Interpreting roles of mutations associated with the emergence of S. aureus USA300 strains using transcriptional regulatory network reconstruction

   Saugat Poudel, Jason Hyun ... Bernhard O Palsson

   Transcriptional regulatory network models can aid in identifying candidate genes associated with the target phenotype in bacterial genome-wide association studies.
9. 1. Neuroscience

   Untangling stability and gain modulation in cortical circuits with multiple interneuron classes

   Hannah Bos, Christoph Miehl ... Brent Doiron

   In recurrently connected cortical networks with different inhibitory neuron subtypes, circuit modulations can increase neuronal gain without compromising network stability.
10. 1. Genetics and Genomics
    2. Neuroscience

    Antagonist actions of CMK-1/CaMKI and TAX-6/calcineurin along the C. elegans thermal avoidance circuit orchestrate adaptation of nociceptive response to repeated stimuli

    Martina Rudgalvyte, Zehan Hu ... Dominique A Glauser

    Phosphoproteomics identifies diverse CaM kinase substrates, while genetic dissection reveals an unexpected antagonistic interaction with calcineurin signaling that operates in distinct neurons to regulate thermo-nociception in Caenorhabditis elegans.
11. 1. Structural Biology and Molecular Biophysics

    Hidden GPCR structural transitions addressed by multiple walker supervised molecular dynamics (mwSuMD)

    Giuseppe Deganutti, Ludovico Pipito ... Christopher Arthur Reynolds

    Multiple walker supervised molecular dynamics simulations deliver insights on glucagon-like peptide 1 receptor activation, G_s protein binding, and guanosine diphosphate release from the G_sα subunit using an adaptive sampling approach.
12. 1. Cell Biology
    2. Cancer Biology

    Tumor Cell Spatial Organization Directs EGFR/RAS/RAF Pathway Primary Therapy Resistance through YAP Signaling

    Rachel Nakagawa, Andrew Beardsley ... Andrei Goga
13. 1. Structural Biology and Molecular Biophysics

    LC8 enhances 53BP1 foci through heterogeneous bridging of 53BP1 oligomers

    Jesse Howe, Douglas Walker ... Elisar J Barbar
14. 1. Neuroscience

    Olfactory ensheathing cells from adult female rats are hybrid glia that promote neural repair

    Patricia E Phelps, Sung Min Ha ... Xia Yang

    To better understand how olfactory ensheathing cells modify spinal cord injury sites, single-cell RNA-sequencing was used to identify five OEC subtypes, progenitors, and novel genes associated with axonal outgrowth.
15. 1. Immunology and Inflammation
    2. Neuroscience

    Microglia aging in the hippocampus advances through intermediate states that drive activation and cognitive decline

    Jeremy M Shea, Saul A Villeda

    During aging, microglia progress through intermediate cellular states, including stress response and translation upregulation states, that modulate subsequent activation and impact cognitive decline.
16. 1. Neuroscience

    A toolbox for ablating excitatory and inhibitory synapses

    Aida Bareghamyan, Changfeng Deng ... Don B Arnold

    Novel genetically encoded tools PFE3, paGFE3, and chGFE3 can be used to reversibly ablate excitatory synapses constitutively, and inhibitory synapses in a light- or chemically dependent manner.
17. 1. Neuroscience

    Leveraging place field repetition to understand positional versus nonpositional inputs to hippocampal field CA1

    William Hockeimer, Ruo-Yah Lai ... James J Knierim

    Place cells that show repeating place fields in geometrically similar locations of an environment modulate their firing rates differently in each field based on movement direction.
18. 1. Genetics and Genomics

    IDH1 regulates human erythropoiesis by eliciting chromatin state reprogramming

    Mengjia Li, Hengchao Zhang ... Lixiang Chen

    Integrated multi-omics analyses reveal the metabolism-independent role of nuclear IDH1 in chromatin remodeling during human erythropoiesis.
19. 1. Ecology

    A circadian clock drives behavioral activity in Antarctic krill (Euphausia superba) and provides a potential mechanism for seasonal timing

    Lukas Hüppe, Dominik Bahlburg ... Bettina Meyer

    Behavioral experiments provide strong evidence that krill swimming behavior is controlled by biological clocks throughout the year, revealing a key mechanism for adaptation to high-latitude habitats.
20. 1. Developmental Biology

    An atypical basement membrane forms a midline barrier during left-right asymmetric gut development in the chicken embryo

    Cora Demler, John C Lawlor ... Natasza A Kurpios

    A novel midline barrier is discovered in the developing gut, highlighting its dynamics and function, with broad implications for patterning mechanisms, extracellular matrix significance, and laterality disorders.
21. 1. Neuroscience

    Rhythmic circuit function is more robust to changes in synaptic than intrinsic conductances

    Zachary Fournier, Leandro M Alonso, Eve Marder

    Modifications of the intrinsic currents in neuronal circuits are more likely to result in loss of function than changes in synaptic strength.
22. 1. Immunology and Inflammation

    Follicular helper- and peripheral helper-like T cells drive autoimmune disease in human immune system mice

    Mohsen Khosravi-Maharlooei, Andrea Vecchione ... Megan Sykes

    Functional analyses identify Tfh-like and Tph-like CD4⁺ T cells as drivers of a spontaneous autoimmune syndrome characterized by autoreactive B cells in human immune system mice.
23. 1. Developmental Biology

    The epididymis contributes to sperm DNA integrity and early embryo development through Cysteine-Rich Secretory Proteins

    Valeria Sulzyk, Ludmila Curci ... Patricia S Cuasnicu

    The identification of epididymal proteins relevant for sperm DNA integrity and early embryo development will contribute to a better understanding, diagnosis, and treatment of human infertility.
24. 1. Computational and Systems Biology

    Barcode-free multiplex plasmid sequencing using Bayesian analysis and nanopore sequencing

    Masaaki Uematsu, Jeremy M Baskin

    SAVEMONEY is a computational tool that guides users to mix plasmids prior to nanopore sequencing, reducing the effective cost of whole-plasmid sequencing to below that of Sanger sequencing.
25. 1. Cancer Biology
    2. Cell Biology

    Changes in local mineral homeostasis facilitate the formation of benign and malignant testicular microcalcifications

    Ida Marie Boisen, Nadia Krarup Knudsen ... Martin Blomberg Jensen

    Testicular microcalcifications arise secondary to local alterations in mineral homeostasis, which in combination with impaired Sertoli cell function and reduced levels of mineralization inhibitors facilitate deposition of hydroxyapatite.
26. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Deep sequencing of yeast and mouse tRNAs and tRNA fragments using OTTR

    Hans Tobias Gustafsson, Lucas Ferguson ... Oliver J Rando
27. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Major nuclear locales define nuclear genome organization and function beyond A and B compartments

    Omid Gholamalamdari, Tom van Schaik ... Andrew S Belmont

    New insights into nuclear genome organization were generated by comparing genome localization relative to three major nuclear locales- nuclear lamina, nuclear speckles, and nucleoli- across several human cell lines.
28. 1. Neuroscience

    Detecting behavioural oscillations with increased sensitivity: A modification of Brookshire’s (2022) AR-surrogate method

    Anthony M Harris, Henry A Beale
29. 1. Neuroscience

    Rhythmic Sampling and Competition of Target and Distractor in a Motion Detection Task

    Changhao Xiong, Nathan M Petro ... Mingzhou Ding
30. 1. Neuroscience

    Task and Behavior-Related Variables Are Encoded by the Postrhinal and Medial Entorhinal Cortex During Non-Spatial Associative Learning

    Ingeborg Nymoen Nystuen, Frederik Sebastian Rogge ... Kristian Kinden Lensjø
31. 1. Computational and Systems Biology

    Unsupervised Representation Learning of C. elegans Poses and Behavior Sequences From Microscope Video Recordings

    Maurice Deserno, Katarzyna Bozek
32. 1. Developmental Biology
    2. Neuroscience

    Bcl11b orchestrates subcerebral projection neuron axon development via cell-autonomous, non-cell-autonomous, and subcellular mechanisms

    Yasuhiro Itoh, Mollie B Woodworth ... Jeffrey D Macklis
33. 1. Developmental Biology
    2. Neuroscience

    Premature vision drives aberrant development of response properties in primary visual cortex

    Sophie V Griswold, Stephen D Van Hooser
34. 1. Immunology and Inflammation

    A host enzyme reduces metabolic dysfunction-associated steatotic liver disease (MASLD) by inactivating intestinal lipopolysaccharide

    Zhiyan Wang, Nore Ojogun ... Mingfang Lu

    A MASLD mouse model reveals that gut-derived Gram-negative bacterial lipopolysaccharides promote hepatic lipid accumulation and acyloxyacyl hydrolase, a host lipase that degrades and inactivates lipopolysaccharides and mitigates MASLD.
35. 1. Neuroscience

    Accelerated signal propagation speed in human neocortical dendrites

    Gáspár Oláh, Rajmund Lákovics ... Gábor Tamás

    Larger dendritic diameter, increased conductance load at the soma, and differences in cable and ion channel properties enable faster signal propagation in human neocortical dendrites, preserving information processing speed.
36. 1. Cell Biology

    Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cells

    Affiong Ika Oqua, Kin Chao ... Alejandra Tomas

    Islet cholesterol levels determine GLP-1R function, with several cholesterol-binding sites mapped in active vs inactive receptors and one, containing Val229, shown to control GLP-1R behaviour in beta cell models.
37. 1. Neuroscience

    Stimulus representation in human frontal cortex supports flexible control in working memory

    Zhujun Shao, Mengya Zhang, Qing Yu

    The distributed network of working memory, encompassing frontal, parietal, and sensory cortices, flexibly reconfigures under different tasks to adapt to varying control demands.
38. 1. Cell Biology
    2. Immunology and Inflammation

    RAS–p110α signalling in macrophages is required for effective inflammatory response and resolution of inflammation

    Alejandro Rosell, Agata Adelajda Krygowska ... Esther Castellano Sanchez

    Disruption of RAS–p110α signalling in macrophages impairs both the initiation and resolution of inflammatory responses, revealing a critical regulatory role in immune function and inflammation control.
39. 1. Cell Biology
    2. Genetics and Genomics

    Pyruvate and related energetic metabolites modulate resilience against high genetic risk for glaucoma

    Keva Li, Nicholas Tolman ... UK Biobank Eye and Vision Consortium

    Pyruvate and related metabolites are linked to reduced glaucoma risk among genetically predisposed individuals and mitigate disease in a mouse model, highlighting a potential metabolic mechanism of resilience.
40. 1. Biochemistry and Chemical Biology

    Stabilization of GTSE1 by cyclin D1–CDK4/6-mediated phosphorylation promotes cell proliferation with implications for cancer prognosis

    Nelson García-Vázquez, Tania J González-Robles ... Michele Pagano

    Cyclin D1–CDK4/6 phosphorylates GTSE1 in cancer cells, preventing its degradation and sustaining high levels across the cell cycle, which enhances proliferation and correlates with poor prognosis.
41. 1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Monoclonal antibodies derived from B cells in subjects with cystic fibrosis reduce Pseudomonas aeruginosa burden in mice

    Malika Hale, Kennidy K Takehara ... Marion Pepper

    Immune memory B cells in people with cystic fibrosis contain bacteria-specific sequences that could be used to make new treatments for life-threatening infections.
42. 1. Neuroscience

    Cerebellar Purkinje cells control posture in larval zebrafish (Danio rerio)

    Franziska Auer, Katherine Nardone ... David Schoppik

    Purkinje cells influence postural control in developing zebrafish, revealing age-dependent cerebellar contributions to balance and establishing a powerful model for studying cerebellar control of postural maturation.
43. 1. Neuroscience

    Resilience of A Learned Motor Behavior After Chronic Disruption of Inhibitory Circuits

    Zsofia Torok, Laura Luebbert ... Carlos Lois
44. 1. Cancer Biology

    Microenvironmental arginine restriction sensitizes pancreatic cancers to polyunsaturated fatty acids by suppression of lipid synthesis

    Patrick B Jonker, Mumina Sadullozoda ... Alexander Muir
45. 1. Immunology and Inflammation
    2. Stem Cells and Regenerative Medicine

    IL-27 limits HSPC differentiation during infection and protects from stem cell exhaustion

    Daniel L Aldridge, Zachary Lanzar ... Christopher A Hunter
46. 1. Neuroscience

    Overt visual attention modulates decision-related signals in ventral and dorsal medial prefrontal cortex

    Blair Shevlin, Rachael Gwinn ... Ian Krajbich
47. 1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Atomistic Simulation of Voltage Activation of a Truncated BK Channel

    Zhiguang Jia, Jianhan Chen
48. 1. Cell Biology
    2. Neuroscience

    CO₂-dependent opening of Connexin 43 hemichannels

    Valentin-Mihai Dospinescu, Alexander Mascarenhas ... Nicholas Dale
49. 1. Chromosomes and Gene Expression

    SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing

    Ashwin Govindan, Nicholas K Conrad

    SFSWAP was identified in a genetic screen to negatively regulate splicing of OGT and other mRNAs primarily by regulating intron retention and exon skipping.
50. 1. Cancer Biology

    TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis

    Qianqian Ju, Wenjing Sheng ... Cheng Sun

    TAK1 plays a negative role in esophageal squamous cancer cell metastasis, which depends on the TAK1-induced phosphorylation of PLCE1 at S1060 and subsequent inhibition of PIP2 hydrolysis.
51. 1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Teichoic acids in the periplasm and cell envelope of Streptococcus pneumoniae

    Mai Nguyen, Elda Bauda ... Cecile Morlot

    The thickness of the periplasmic region is reduced in strains of pneumococcus that are deleted in genes responsible for the incorporation of teichoic acid in the cell envelope.
52. 1. Medicine
    2. Neuroscience

    Simply crushed zizyphi spinosi semen prevents neurodegenerative diseases and reverses age-related cognitive decline in mice

    Tomohiro Umeda, Ayumi Sakai ... Takami Tomiyama

    Simply crushed zizyphi spinosi semen not only ameliorates Aβ, tau, and α-synuclein pathology in dementia model mice, but also rejuvenates brain function by suppressing cellular senescence in normal aged mice.
53. 1. Medicine
    2. Neuroscience

    Diet modulates the therapeutic effects of dimethyl fumarate mediated by the immunometabolic neutrophil receptor HCAR

    Joanna Kosinska, Julian C Assmann ... Markus Schwaninger
54. 1. Neuroscience

    Elevated pyramidal cell firing orchestrates arteriolar vasoconstriction through COX-2-derived prostaglandin E2 signaling

    Benjamin Le Gac, Marine Tournissac ... Bruno Cauli

    Increased neuronal activity of cortical pyramidal cells can paradoxically reduce cerebral blood flow via vasoconstriction mediated by glutamate and lipid messengers.
55. 1. Microbiology and Infectious Disease

    Purging viral latency by a bifunctional HSV-vectored therapeutic vaccine in chronically SIV-infected macaques

    Ziyu Wen, Pingchao Li ... Caijun Sun

    HSV-ΔICP34.5 reactivates latent HIV by modulating PP1-HSF1 and NF-κB pathways, offering a potential HSV-vectored therapeutic vaccine for a functional cure.
56. 1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry

    Douwe Schulte, Marta Šiborová ... Joost Snijder

    Germline V-gene usage can be inferred from cryoEM reconstructions of antigen-antibody complexes to guide de novo antibody sequencing of complex mixtures by mass spectrometry.
57. 1. Neuroscience

    APP β-CTF triggers cell-autonomous synaptic toxicity independent of Aβ

    Menguxn Luo, Jia Zhou ... Yelin Chen

    β-CTF of APP, not Aβ, induces synaptic loss in a cell-autonomous manner, revealing APP misregulation may contribute to the pathogenesis of Alzheimer's disease via an Aβ-independent mechanism.
58. 1. Developmental Biology
    2. Genetics and Genomics

    Single-cell transcriptomics of X-ray irradiated Drosophila wing discs reveals heterogeneity related to cell-cycle status and cell location

    Joyner Cruz, Willam Y Sun ... Iswar K Hariharan
59. 1. Cell Biology
    2. Cancer Biology

    DuoHexaBody-CD37 induces direct cytotoxic signaling in diffuse large B-cell lymphoma

    Simar Pal Singh, Michelle D van den Beukel ... Annemiek B van Spriel
60. 1. Neuroscience

    Mouse sensorimotor cortex reflects complex kinematic details during reaching and grasping

    Harrison A Grier, Sohrab Salimian, Matthew T Kaufman
61. 1. Neuroscience

    A high-throughput approach for the efficient prediction of perceived similarity of natural objects

    Philipp Kaniuth, Florian P Mahner ... Martin N Hebart
62. 1. Chromosomes and Gene Expression

    Identification of the Regulatory Elements and Protein Substrates of Lysine Acetoacetylation

    Qianyun Fu, Terry Nguyen ... Y George Zheng
63. 1. Microbiology and Infectious Disease

    Secreted small RNAs of Naegleria fowleri are biomarkers for diagnosis of primary amoebic meningoencephalitis

    A Cassiopeia Russell, Joseph Dainis ... Dennis E Kyle
64. 1. Neuroscience

    Nocebo effects are stronger and more persistent than placebo effects in healthy individuals

    Angelika Kunkel, Katharina Schmidt ... Ulrike Bingel
65. 1. Immunology and Inflammation

    MicroRNA-26b protects against MASH development in mice and can be efficiently targeted with lipid nanoparticles

    Linsey Peters, Leonida Rakateli ... Emiel van der Vorst

    Lipid nanoparticles containing microRNA-26-mimics can reduce MASH in mice, providing a novel therapeutic approach.
66. 1. Physics of Living Systems

    Modeling collective behavior in groups of mice housed under semi-naturalistic conditions

    Xiaowen Chen, Maciej Winiarksi ... Aleksandra M Walczak

    The social structure of interactions in freely behaving animals predicts the effects of perturbing the neural plasticity of one individual on group sociability.
67. 1. Neuroscience

    Auditory cortex learns to discriminate audiovisual cues through selective multisensory enhancement

    Song Chang, Beilin Zheng ... Liping Yu

    Experience-driven plasticity in auditory cortex neurons establishes cross-modal associations, enabling enhanced discrimination of multisensory cues through specialized integrative strategies.
68. 1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins

    Shinichi Kawaguchi, Xin Xu ... Toshie Kai

    AlphaFold2-based large-scale screening reveals novel protein interactions, accelerating functional studies of piRNA pathway proteins and expanding bioinformatic-experimental integration.
69. 1. Evolutionary Biology

    Pronounced expression of extracellular matrix proteoglycans regulated by Wnt pathway underlies the parallel evolution of lip hypertrophy in East African cichlids

    Nagatoshi Machii, Ryo Hatashima ... Masato Nikaido

    A shared molecular basis of proteoglycan accumulation, driven by Wnt signaling, underlies the parallel evolution of hypertrophied lips in East African cichlids.
70. 1. Neuroscience

    Re-focusing visual working memory during expected and unexpected memory tests

    Sisi Wang, Freek van Ede

    Memory tests evoke a second stage of internal attentional deployment following both expected and unexpected memory tests, extending attentional re-orienting to working memory.
71. 1. Cancer Biology
    2. Cell Biology

    Cell crowding activates pro-invasive mechanotransduction pathway in high-grade DCIS via TRPV4 inhibition and cell volume reduction

    Xiangning Bu, Nathanael Ashby ... Inhee Chung

    Cell crowding drives a pro-invasive mechanotransduction pathway in high-grade DCIS, inducing TRPV4 inhibition, calcium and cell volume reduction, and increased invasion, with compensatory plasma-membrane TRPV4 relocation indicating pathway activation.
72. 1. Neuroscience

    Semantic representations in the visual cortex of blind and sighted humans

    Małgorzata Paczyńska, Marta Urbaniak ... Łukasz Bola
73. 1. Neuroscience

    Auditory stimuli extend the temporal window of visual integration by modulating alpha-band oscillations

    Mengting Xu, Biao Han ... Lu Shen
74. 1. Neuroscience

    Stimulus dependencies—rather than next-word prediction—can explain pre-onset brain encoding during natural listening

    Inés Schönmann, Jakub Szewczyk ... Micha Heilbron
75. 1. Cancer Biology

    Δ133p53α and Δ160p53α isoforms of the tumor suppressor protein p53 exert dominant-negative effect primarily by co-aggregation

    Liuqun Zhao, Tanel Punga, Suparna Sanyal
76. 1. Genetics and Genomics

    Convergent evolution of epigenome recruited DNA repair across the Tree of Life

    J Grey Monroe, Chaehee Lee ... Daniel Runcie
77. 1. Neuroscience

    Modulation of brain signal variability in visual cortex reflects aging, GABA, and behavior

    Poortata Lalwani, Thad Polk, Douglas D Garrett

    Humans scale brain signal variability with stimulus complexity to efficiently process visual inputs, an ability reduced in those with poorer visual discrimination and in older adults with lower GABA levels (brain's major inhibitory neurotransmitter), but recoverable by boosting GABA pharmacologically.
78. 1. Neuroscience

    Cholesterol taste avoidance in Drosophila melanogaster

    Roshani Nhuchhen Pradhan, Craig Montell, Youngseok Lee

    Fruit flies detect cholesterol through ionotropic receptors in taste, leading to active avoidance despite dietary necessity.
79. 1. Cell Biology

    Targeting IRE1α improves insulin sensitivity and thermogenesis and suppresses metabolically active adipose tissue macrophages in male obese mice

    Dan Wu, Venkateswararao Eeda ... Weidong Wang

    Diet-induced obesity, insulin resistance, and proinflammatory macrophage activation and accumulation in adipose tissue can be counteracted through the inhibition of ER stress sensor IRE1alpha.
80. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Kinetic regulation of kinesin’s two motor domains coordinates its stepping along microtubules

    Yamato Niitani, Kohei Matsuzaki ... Michio Tomishige

    Kinetic measurements of kinesin-1's front and rear heads reveal how different neck linker orientation regulate their activity, enabling the motor protein to move in a coordinated manner along microtubules.
81. 1. Developmental Biology

    Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation

    Satoshi Yamashita, Shuji Ishihara, François Graner

    A physical modeling and its simulation showed distinctive role of surface elasticity and contractility during the apical constriction.
82. 1. Neuroscience

    Shaping the physical world to our ends through the left PF technical-cognition area

    François Osiurak, Giovanni Federico ... Mathieu Lesourd

    Understanding and reasoning about the physical world rely on a specific brain network, in which the area PF of the left inferior parietal lobe plays a central role.
83. 1. Evolutionary Biology

    Still waters run deep in large-scale genome rearrangements of morphologically conservative Polyplacophora

    Julia D Sigwart, Yunlong Li ... Jin Sun

    Chitons overcome evolutionary constraints through unprecedented genomic dynamism, with extensive chromosomal rearrangements and duplications despite about 300 million years of morphological stasis.
84. 1. Cancer Biology
    2. Genetics and Genomics

    Combinatorial CRISPR screen reveals FYN and KDM4 as targets for synergistic drug combination for treating triple negative breast cancer

    Tackhoon Kim, Byung-Sun Park ... Timothy Lu

    Pairwise CRISPR screen identified FYN and KDM4 as key regulator of tyrosine kinase inhibitor resistance in triple negative breast cancer (TNBC).
85. 1. Evolutionary Biology

    Social and environmental predictors of gut microbiome age in wild baboons

    Mauna R Dasari, Kimberly E Roche ... Elizabeth A Archie

    The mammalian gut microbiome can be used as a noninvasive and holistic predictor of biological age.
86. 1. Cell Biology

    TRPγ regulates lipid metabolism through Dh44 neuroendocrine cells

    Dharmendra Kumar Nath, Subash Dhakal, Youngseok Lee

    TRPγ is essential for maintaining lipid homeostasis in Drosophila melanogaster, linking neuronal regulation to fat metabolism and suggesting potential therapeutic avenues for metabolic disorders.
87. 1. Genetics and Genomics
    2. Neuroscience

    Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

    Tanya Wolff, Mark Eddison ... Gerald M Rubin

    Genetic tools were generated and used to determine the neurotransmitters and neuropeptides used by individual cell types within the Drosophila central complex and to study their roles in sleep regulation.
88. 1. Neuroscience

    A general framework for characterizing optimal communication in brain networks

    Kayson Fakhar, Fatemeh Hadaeghi ... Claus C Hilgetag

    A game-theoretical framework for uncovering how optimal signal transmission takes place in a given network, here applied to large-scale brain networks.
89. 1. Immunology and Inflammation

    scRNA+TCR-seq Reveals the Proportion and Characteristics of Dual TCR Treg Cells in Mouse Lymphoid and Non-lymphoid Tissues

    Yuanyuan Xu, Qipeng ... Xinsheng Yao
90. 1. Cancer Biology
    2. Evolutionary Biology

    Cancer-immune coevolution dictated by antigenic mutation accumulation

    Long Wang, Christo Morison, Weini Huang
91. 1. Neuroscience

    Interdigitating Modules for Visual Processing During Locomotion and Rest in Mouse V1

    Andrew M Meier, Rinaldo D D’Souza ... Andreas Burkhalter
92. 1. Neuroscience

    High Cognitive Violation of Expectations is Compromised in Cerebellar Ataxia

    Leonardo Daniel A, Eli Vakil, William Saban
93. 1. Evolutionary Biology

    The population structure of invasive Lantana camara is shaped by its mating system

    P Praveen, Rajesh Gopal, Uma Ramakrishnan
94. 1. Cancer Biology
    2. Genetics and Genomics

    Functional characterization of all CDKN2A missense variants and comparison to in silico models of pathogenicity

    Hirokazu Kimura, Kamel Lahouel ... Nicholas Jason Roberts

    High-throughput characterization of all possible CDKN2A missense variants identifies functionally deleterious variants and establishes accuracy of variant effect predictors.
95. 1. Neuroscience

    Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses

    Lucie Oriol, Melody Chao ... Thomas S Hnasko

    Putative markers of VTA interneurons label VTA projection neurons, and VTA projection neurons make intra-VTA synapses, suggesting VTA projection neurons may mediate functions prior attributed to interneurons.
96. 1. Neuroscience

    A neurocomputational account of the link between social perception and social action

    Lisa M Bas, Ian D Roberts ... Anita Tusche

    Future prosocial acts can be forecasted from stable biological differences in how people perceive others' merit, pointing to novel strategies to reduce favoritism and discrimination in social situations.
97. 1. Evolutionary Biology
    2. Genetics and Genomics

    The potential of inversions to accumulate balanced sexual antagonism is supported by simulations and Drosophila experiments

    Christopher S McAllester, John E Pool

    Sexually antagonistic pleiotropy involving frequency-dependent traits like mating display maintains linkage blocks like inversions as balanced polymorphisms in simulation, and may explain common inversion polymorphisms maintained at intermediate frequencies.
98. 1. Physics of Living Systems

    Differential spatial regulation and activation of integrin nanoclusters inside focal adhesions

    Sarah Keary, Nicolas Mateos ... Maria F Garcia-Parajo
99. 1. Neuroscience

    Neural signatures of model-based and model-free reinforcement learning across prefrontal cortex and striatum

    Bruno Miranda, James L Butler ... Steven W Kennerley
100. 1. Cell Biology

     Nim1-related kinases regulate septin organization and cytokinesis by modulating Hof1 at the cell division site

     Bindu Bhojappa, Anubhav Dhar ... Saravanan Palani
101. 1. Physics of Living Systems

     Quantifying the shape of cells - from Minkowski tensors to p-atic order

     Lea Happel, Griseldis Oberschelp ... Axel Voigt
102. 1. Biochemistry and Chemical Biology

     Phage-displayed synthetic library and screening platform for nanobody discovery

     Baolong Xia, Ah-Ram Kim ... Norbert Perrimon
103. 1. Neuroscience

     Neuropeptidergic circuit modulation of developmental sleep in Drosophila

     Chikayo Hemmi, Kenichi Ishii ... Kazuo Emoto
104. 1. Genetics and Genomics
     2. Computational and Systems Biology

     Imputation of structural variants using a multi-ancestry long-read sequencing panel enables identification of disease associations

     Boris Noyvert, A Mesut Erzurumluoglu ... Zhihao Ding
105. 1. Genetics and Genomics

     Dynamics of transcriptional programs and chromatin accessibility in mouse spermatogonial cells from early postnatal to adult life

     Irina Lazar-Contes, Rodrigo G Arzate-Mejia ... Isabelle M Mansuy

     Developmental transitions in mouse spermatogonial cells reveal extensive chromatin remodeling, stage-specific gene expression, and newly accessible transposable elements with regulatory potential.
106. 1. Cell Biology
     2. Immunology and Inflammation

     UFMTrack, an Under-Flow Migration Tracker enabling analysis of the entire multi-step immune cell extravasation cascade across the blood-brain barrier in microfluidic devices

     Mykhailo Vladymyrov, Luca Marchetti ... Britta Engelhardt

     The established under-flow migration tracker (^UFMTrack) framework allows for automated and scalable studies of immune cell interactions with and extravasation across endothelial monolayer under flow in vitro.
107. 1. Neuroscience

     An anatomical and physiological basis for flexible coincidence detection in the auditory system

     Lauren J Kreeger, Suraj Honnuraiah ... Lisa Goodrich

     In the mammalian auditory brainstem, inhibitory inputs onto octopus cell dendrites enhance coincidence detection computations and support precise yet flexible temporal processing for rapid sound onsets and frequency modulations.
108. 1. Immunology and Inflammation

     The extra-islet pancreas supports autoimmunity in human type 1 diabetes

     Graham L Barlow, Christian M Schürch ... Paul L Bollyky

     A high-parameter, spatial analysis of the pancreata of individuals with type 1 diabetes reveals specific patterns of immune infiltration into islets of Langerhans, coordinated with changes in the surrounding tissue.
109. 1. Immunology and Inflammation

     Coevolutionary interplay: Helminths-trained immunity and its impact on the rise of inflammatory diseases

     Eugenio Antonio Carrera Silva, Juliana Puyssegur, Andrea Emilse Errasti

     Deciphering the gut biome's, including helminths', influence on human trained immunity will unlock novel immunotherapies harnessing the immune reprogramming potential of their secreted factors and extracellular vesicles.
110. 1. Structural Biology and Molecular Biophysics

     Distinct activation mechanisms of CXCR4 and ACKR3 revealed by single-molecule analysis of their conformational landscapes

     Christopher T Schafer, Raymond F Pauszek III ... David P Millar

     The conformational dynamics and landscapes of the related chemokine receptors CXCR4 and ACKR3, revealed by single-molecule analysis, provide mechanistic insights into differences in constitutive activity, ligand promiscuity, and effector coupling.
111. 1. Neuroscience

     Neural mechanisms of credit assignment for delayed outcomes during contingent learning

     Phillip P Witkowski, Lindsay JH Rondot ... Erie Boorman

     Orbitofrontal cortex and hippocampus reinstate representations of causal choices to associate with delayed outcomes, and the frontal pole supports this credit assignment process by maintaining pending choices during interim decisions.
112. 1. Neuroscience

     Non-allometric expansion and enhanced compartmentalization of Purkinje cell dendrites in the human cerebellum

     Silas E Busch, Christian Hansel

     A comparative study of Purkinje dendrite morphology, input arrangement, and regional subtype distribution shows human cells evade constraint by cortical thickness to be both quantitatively and qualitatively distinct from mouse.
113. 1. Medicine

     Sex-specific attenuation of photoreceptor degeneration by reserpine in a rhodopsin P23H rat model of autosomal dominant retinitis pigmentosa

     Hyun Beom Song, Laura Campello ... Anand Swaroop

     Reserpine, previously identified through high-throughput screening in CEP290 retinal degeneration models, enhances photoreceptor survival in P23H rhodopsin retinal degeneration models, particularly in female rats, indicating its gene-agnostic and sex-specific effectiveness.
114. 1. Microbiology and Infectious Disease

     Serial ‘deep-sampling’ PCR of fragmented DNA reveals the wide range of Trypanosoma cruzi burden among chronically infected human, macaque, and canine hosts, and allows accurate monitoring of parasite load following treatment

     Brooke E White, Carolyn L Hodo ... Rick L Tarleton

     A vastly improved PCR-based approach provides a long-sought test of cure and parasite load monitoring tool for Chagas disease.
115. 1. Neuroscience

     Adult Neurogenesis Reconciles Flexibility and Stability of Olfactory Perceptual Memory

     Bennet Sakelaris, Hermann Riecke
116. 1. Neuroscience

     Precision cutaneous stimulation in freely moving mice

     Isobel Parkes, Ara Schorscher-Petcu ... Liam E Browne
117. 1. Physics of Living Systems

     Design principles of transcription factors with intrinsically disordered regions

     Wencheng Ji, Ori Hachmo ... Ariel Amir
118. 1. Neuroscience

     A unified rodent atlas reveals the cellular complexity and evolutionary divergence of the dorsal vagal complex

     Cecilia Hes, Abigail J Tomlinson ... Paul V Sabatini
119. 1. Developmental Biology
     2. Evolutionary Biology

     Developmental, regenerative, and behavioral dynamics in acoel reproduction

     Vikram Chandra, Samantha E Tseng ... Mansi Srivastava
120. 1. Physics of Living Systems

     Cell cycle and age-related modulations of mouse chromosome stiffness

     Ning Liu, Wenan Qiang ... Huanyu Qiao

     Mammalian condensed chromosomes exhibit diverse structural properties, as reflected in their varying stiffness across different cell stages and with aging.
121. 1. Microbiology and Infectious Disease
     2. Neuroscience

     Enteric glia regulate Paneth cell secretion and intestinal microbial ecology

     Aleksandra Prochera, Anoohya N Muppirala ... Meenakshi Rao

     Genetic depletion of enteric glia in vivo disrupts antimicrobial peptide secretion by Paneth cells to change fecal microbial composition but does not broadly alter other intestinal programs.
122. 1. Cell Biology
     2. Chromosomes and Gene Expression

     Treacle’s ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair

     Artem K Velichko, Nadezhda V Petrova ... Omar L Kantidze

     Treacle condensation, driven by alternating charge blocks, facilitates interactions with transcription factors to spatially segregate ribosomal RNA synthesis and processing, while also recruiting TOPBP1 for activation of the rDNA damage response.
123. 1. Neuroscience

     Somatodendritic orientation determines tDCS-induced neuromodulation of Purkinje cell activity in awake mice

     Carlos A Sánchez-León, Guillermo Sánchez-Garrido Campos ... Javier Márquez-Ruiz

     The neuromodulatory effects of cerebellar tDCS depend on Purkinje cell somatodendritic orientation relative to the electric field, revealing a key factor for optimizing stimulation protocols and computational models.
124. 1. Evolutionary Biology

     Prophage-encoded Hm-oscar gene recapitulates Wolbachia-induced male-killing in the tea tortrix moth Homona magnanima

     Hiroshi Arai, Susumu Katsuma ... Daisuke Kageyama

     Oscar homologs play a conserved role in Wolbachia-induced male-killing in Lepidoptera but not in other insects, highlighting the evolution of diverse male-killing mechanisms induced by Wolbachia.
125. 1. Neuroscience

     Food intake enhances hippocampal sharp wave-ripples

     Ekin Kaya, Evan Wegienka ... Gideon Rothschild

     Hippocampal sharp wave-ripples during sleep are enhanced by food intake in a calorie-dependent manner and are associated with increased activity of lateral hypothalamic GABAergic neurons.
126. 1. Evolutionary Biology

     Pleiotropy increases parallel selection signatures during adaptation from standing genetic variation

     Wei-Yun Lai, Sheng-Kai Hsu ... Christian Schlötterer

     Evolved gene expression changes in Drosophila simulans uncovered the interplay between parallel evolution, polymorphism, and pleiotropy and indicated that both direct and indirect effects of pleiotropy contribute to parallel evolution.
127. 1. Cancer Biology
     2. Developmental Biology

     Oncogenic and teratogenic effects of Trp53^Y217C, an inflammation-prone mouse model of the human hotspot mutant TP53^Y220C

     Sara Jaber, Eliana Eldawra ... Franck Toledo

     In a mouse model of the hotspot mutant TP53^Y220C, the mutant p53 exhibited oncogenic gain of function in males and teratogenic gain of function in females, both correlated with inflammation.
128. 1. Neuroscience

     Regional heterogeneities of oligodendrocytes determine biased distribution pattern of Ranvier nodes along single axons in sound localization circuit

     Ryo Egawa, Kota Hiraga ... Hiroshi Kuba
129. 1. Physics of Living Systems

     Endurance Exercise Ameliorates Aging-Related Bradyarrhythmia in Drosophila Resulting from miR-283 Knockdown in LN_vs

     Qiufang Li, Xu Ping ... Lan Zheng
130. 1. Neuroscience

     Human single-neuron activity is modulated by intracranial theta burst stimulation of the basolateral amygdala

     Justin M Campbell, Rhiannon L Cowan ... Jon T Willie
131. 1. Neuroscience

     Specificity Protein 1 is essential for the limb trajectory of ephrin-mediated spinal motor axons

     Pinwen Liao, Ming-Yuan Chang ... Tzu-Jen Kao
132. 1. Neuroscience

     A cross-species framework for investigating perceptual evidence accumulation

     Sucheta Chakravarty, Cristina Delgado-Sallent ... Benjamin B Scott
133. 1. Neuroscience

     Individual dopaminergic neurons induce unique, yet overlapping combinations of behavioural modulations including safety learning, memory retrieval and acute locomotion

     Naoko Toshima, Arman Behrad ... Michael Schleyer
134. 1. Chromosomes and Gene Expression
     2. Physics of Living Systems

     A mathematical model clarifies the ABC Score formula used in enhancer-gene prediction

     Joseph Nasser, Kee-Myoung Nam, Jeremy Gunawardena
135. 1. Physics of Living Systems

     Microbiomes Through the Looking Glass

     Jacopo Pasqualini, Amos Maritan ... Samir Suweis
136. 1. Neuroscience

     AFD Thermosensory Neurons Mediate Tactile-Dependent Locomotion Modulation in C. elegans

     Manuel Rosero, Jihong Bai
137. 1. Genetics and Genomics
     2. Neuroscience

     Integrating bulk and single cell RNA-seq refines transcriptomic profiles of individual C. elegans neurons

     Alec Barrett, Erdem Varol ... Marc Hammarlund
138. 1. Cancer Biology

     Loss function of tumor suppressor FRMD8 confers resistance to tamoxifen therapy via a dual mechanism

     Weijie Wu, Miao Yu ... Hongquan Zhang

     Tumor-suppressive protein FRMD8 inhibits breast cancer progression by regulating the level of ERα and has the possibility to be a potential target for overcoming tamoxifen treatment resistance.
139. 1. Neuroscience

     Mechanisms that regulate the C1-C2B mutual inhibition control functional switch of UNC-13

     Haowen Liu, Lei Li ... Zhitao Hu

     A novel mechanistic mechanism in UNC-13/Munc13 regulates synaptic neurotransmitter release at the Caenorhabditis elegans neuromuscular junction.
140. 1. Neuroscience

     Diverging roles of TRPV1 and TRPM2 in warm-temperature detection

     Muad Y Abd El Hay, Gretel B Kamm ... Jan Siemens

     Different aspects of thermal stimuli, such as speed and intensity, are detected by distinct TRP channel-based mechanisms, with TRPV1 and TRPM2 playing unique roles in warmth perception and thermal decision-making.
141. 1. Evolutionary Biology

     Developing a crop- wild-reservoir pathogen system to understand pathogen evolution and emergence

     Mark McMullan, Lawrence Percival-Alwyn ... Neil Hall

     Evidence for those pathogen genes selected specifically for success on crops.
142. 1. Neuroscience

     Dynamic gamma modulation of hippocampal place cells predominates development of theta sequences

     Ning Wang, Yimeng Wang ... Dong Ming

     A subset of hippocampal place cells, which is phase-locked to fast gamma rhythms, compressively encodes information at precessing slow gamma phases and therefore predominates to theta sequence development.
143. 1. Cell Biology
     2. Developmental Biology

     Gene regulatory mechanisms guiding bifurcation of inhibitory and excitatory neuron lineages in the anterior brainstem

     Sami Kilpinen, Lassi Virtanen ... Juha Partanen
144. 1. Cell Biology
     2. Microbiology and Infectious Disease

     A novel SUN1-ALLAN complex coordinates segregation of the bipartite MTOC across the nuclear envelope during rapid closed mitosis in Plasmodium

     Mohammad Zeeshan, Igor Blatov ... Rita Tewari
145. 1. Neuroscience

     A behavioral architecture for realistic simulations of Drosophila larva locomotion and foraging

     Panagiotis Sakagiannis, Anna-Maria Jürgensen, Martin Paul Nawrot
146. 1. Neuroscience

     Active vision of bees in a simple pattern discrimination task

     HaDi MaBouDi, Jasmin Richter ... Lars Chittka

     Bumblebees refine visual discrimination through selective and structured scanning, demonstrating a dynamic interplay between movement and perception in pattern recognition.
147. 1. Microbiology and Infectious Disease

     Integrator complex subunit 12 knockout overcomes a transcriptional block to HIV latency reversal

     Carley N Gray, Manickam Ashokkumar ... Michael Emerman

     A block to more potent and specific release of HIV from latency has been identified as a subunit of the Integrator complex, which may aid strategies for HIV cure.
148. 1. Cancer Biology

     STAMBPL1 activates the GRHL3/HIF1A/VEGFA axis through interaction with FOXO1 to promote angiogenesis in triple-negative breast cancer

     Huan Fang, Huichun Liang ... Ceshi Chen

     STAMPBL1 activates the transcription of GRHL3 by interacting with FOXO1 in nucleus, thereby upregulating the HIF1α/VEGFA axis and promoting angiogenesis in TNBC tumors, independently of its DUB activity.
149. 1. Biochemistry and Chemical Biology
     2. Computational and Systems Biology

     Allosteric modulation by the fatty acid site in the glycosylated SARS-CoV-2 spike

     A Sofia F Oliveira, Fiona L Kearns ... Adrian J Mulholland

     Equilibrium and nonequilibrium simulations uncover how the fatty acid site in the SARS-CoV-2 spike modulates protein dynamics, revealing its intricate allosteric connections and the impact of glycans on these networks.
150. 1. Cell Biology
     2. Medicine

     Adventitial fibroblasts direct smooth muscle cell-state transition in pulmonary vascular disease

     Slaven Crnkovic, Helene Thekkekara Puthenparampil ... Grazyna Kwapiszewska

     Transition from healthy to diseased state is a cell-type-specific process, influenced by neighboring cells and resulting in functionally meaningful differences between pulmonary artery adventitial fibroblasts and smooth muscle cells.
151. 1. Medicine

     Transplantation of exogenous mitochondria mitigates myocardial dysfunction after cardiac arrest

     Zhen Wang, Jie Zhu ... Xiang Zhou

     Exogenous mitochondrial transplantation improves cardiac function after cardiopulmonary resuscitation (CPR) may be related to improving mitochondrial function, reducing the oxidative stress response, and decreasing apoptosis in myocardial cells.
152. 1. Genetics and Genomics

     Trade-offs in modeling context dependency in complex trait genetics

     Eric Weine, Samuel Pattillo Smith ... Arbel Harpak

     The estimation of individual genetic effects across different contexts can be boiled down to a bias-variance trade-off, yet for complex traits, the joint consideration of numerous causal effects alters this trade-off.
153. 1. Cancer Biology
     2. Physics of Living Systems

     Mechanistic insight for T-cell exclusion by cancer-associated fibroblasts in human lung cancer

     Joseph Ackermann, Chiara Bernard ... Martine D Ben Amar

     The crosstalk between cancerous lesions and the immune system in the lungs demonstrates the complex role of fibroblasts.
154. 1. Neuroscience

     Type-I nNOS neurons orchestrate cortical neural activity and vasomotion

     Kevin L Turner, Dakota F Brockway ... Patrick J Drew
155. 1. Neuroscience

     Gender–specific Single Transcript Level Atlas of Vasopressin and its Receptor (AVPR1a) in the Mouse Brain

     Anisa Gumerova, Georgii Pevnev ... Vitaly Ryu
156. 1. Cell Biology
     2. Genetics and Genomics

     Heme’s relevance genuine? Re-visiting the roles of TANGO2 homologs including HRG-9 and HRG-10 in C. elegans

     Sarah E Sandkuhler, Kayla S Youngs ... Samuel J Mackenzie
157. 1. Evolutionary Biology

     Nitrogenase structural evolution across Earth’s history

     Bruno Cuevas-Zuviría, Franka Detemple ... Betül Kaçar
158. 1. Developmental Biology

     Site-specific DNA demethylation during spermatogenesis presets the sites of nucleosome retention in mouse sperm

     So Maezawa, Masashi Yukawa ... Satoshi H Namekawa
159. 1. Neuroscience

     Top-down feedback matters: Functional impact of brainlike connectivity motifs on audiovisual integration

     Mashbayar Tugsbayar, Mingze Li ... Blake Richards
160. 1. Cancer Biology
     2. Cell Biology

     Cytosolic and endoplasmic reticulum chaperones inhibit wt-p53 to increase cancer cells' survival by refluxing ER-proteins to the cytosol

     Salam Dabsan, Gali Zur ... Aeid Igbaria

     Identifying the regulators of a non-genetic mechanism (ERCYS) that is constitutively activated in cancer cells to provide pro-survival properties.
161. 1. Neuroscience

     Repeated activation of preoptic area recipient neurons in posterior paraventricular nucleus mediates chronic heat-induced negative emotional valence and hyperarousal states

     Zhiping Cao, Wing-Ho Yung, Ya Ke

     A neural circuit mechanism explains how chronic extreme heat exposure affects emotions in the mouse model.
162. 1. Developmental Biology

     Numb provides a fail-safe mechanism for intestinal stem cell self-renewal in adult Drosophila midgut

     Mengjie Li, Aiguo Tian, Jin Jiang

     The asymmetrically segregated Notch pathway inhibitor Numb is essential for intestinal stem cell self-renewal in adult Drosophila midguts when the stem cell niche signaling is compromised.
163. 1. Biochemistry and Chemical Biology
     2. Genetics and Genomics

     High-resolution deep mutational scanning of the melanocortin-4 receptor enables target characterization for drug discovery

     Conor J Howard, Nathan S Abell ... Nathan B Lubock

     Mapping the functional landscape of >6600 MC4R variants via deep mutational scanning reveals novel determinants of biased signaling and ligand interactions that inform targeted obesity therapeutics.
164. 1. Neuroscience
     2. Stem Cells and Regenerative Medicine

     Master control genes in the regeneration of rod photoreceptors from endogenous progenitor cells in zebrafish retina

     Eyad Shihabeddin, Abirami Santhanam ... John O’Brien
165. 1. Neuroscience

     Neocortical Layer-5 tLTD Relies on Non-Ionotropic Presynaptic NMDA Receptor Signaling

     Aurore Thomazeau, Sabine Rannio ... P Jesper Sjöström
166. 1. Neuroscience

     Separable Dorsal Raphe Dopamine Projections mediate the Facets of Loneliness-like state

     Christopher R Lee, Gillian A Matthews ... Kay M Tye
167. 1. Neuroscience

     Audiovisual cues must be predictable and win-paired to drive risky choice

     Brett A Hathaway, Dexter R Kim ... Catharine A Winstanley
168. 1. Neuroscience
     2. Computational and Systems Biology

     Predictive modeling of hematoma expansion from non-contrast computed tomography in spontaneous intracerebral hemorrhage patients

     Natasha Ironside, Kareem El Naamani ... ERICH Investigators and the VISTA-ICH collaboration
169. 1. Neuroscience

     The coarse mental map of the breast is anchored on the nipple

     Katie H Long, Emily E Fitzgerald ... Charles M Greenspon
170. 1. Neuroscience

     Negative affect influences the computations underlying food choice in bulimia nervosa

     Blair RK Shevlin, Loren Gianini ... Laura A Berner
171. 1. Medicine

     Dynamics of compartment-specific proteomic landscapes of hepatotoxic and cholestatic models of liver fibrosis

     Marketa Jirouskova, Karel Harant ... Martin Gregor

     A comprehensive proteomic map of liver fibrosis progression and healing in two mouse models identifies etiology-specific extracellular matrix components, laying the foundation for targeted antifibrotic therapies.
172. 1. Biochemistry and Chemical Biology
     2. Genetics and Genomics

     Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing

     Jiale Zhou, Ding Zhao ... Zhanjun Li

     The DRAM system enables antibody-free, bisulfite-free, and transcriptome-wide mapping of 5-methylcytosine in mRNA with high accuracy, stability, and sensitivity, even at ultralow RNA inputs.
173. 1. Ecology

     Connecting the dots: Managing species interaction networks to mitigate the impacts of global change

     Luis Abdala-Roberts, Adriana Puentes ... Kailen A Mooney

     Ecological network management provides key mechanistic knowledge on ecosystem degradation that links species- to ecosystem-level responses to global change, and emerging technological tools offer the opportunity to accelerate its widespread adoption.
174. 1. Biochemistry and Chemical Biology

     Coordinated regulation of chemotaxis and resistance to copper by CsoR in Pseudomonas putida

     Meina He, Yongxin Tao ... Wenli Chen

     Pseudomonas putida coordinates chemotaxis and resistance to copper through the interaction between CheA and CsoR.
175. 1. Immunology and Inflammation

     Sex-dependent gastrointestinal colonization resistance to MRSA is microbiota and Th17 dependent

     Alannah Lejeune, Chunyi Zhou ... Ken Cadwell

     Gut microbiota composition and sex hormones influence the immune response to methicillin-resistant Staphylococcus aureus gastrointestinal colonization in mice.
176. 1. Developmental Biology

     A single-cell atlas of spatial and temporal gene expression in the mouse cranial neural plate

     Eric R Brooks, Andrew R Moorman ... Jennifer A Zallen

     Single-cell RNA sequencing and computational analysis define a spatial and temporal map of gene expression during early patterning and morphogenesis of the mouse cranial neural plate, providing a resource for elucidating the transcriptional basis of mammalian brain development.
177. 1. Medicine
     2. Neuroscience

     Preclinical systematic review of CCR5 antagonists as cerebroprotective and stroke recovery enhancing agents

     Ayni Sharif, Matthew S Jeffers ... Manoj M Lalu

     CCR5 antagonists show promise for stroke cerebroprotection and recovery, although further preclinical evidence in clinically relevant domains could strengthen support for clinical translation.
178. 1. Evolutionary Biology
     2. Neuroscience

     Cellular evolution of the hypothalamic preoptic area of behaviorally divergent deer mice

     Jenny Chen, Phoebe R Richardson ... Hopi E Hoekstra

     Single-nucleus RNA-sequencing of the hypothalamic preoptic area of monogamous and promiscuous deer mouse species reveals neuronal differences that may be responsible for innate changes in mating and parental care behavior.
179. 1. Cancer Biology
     2. Cell Biology

     N6-methyladenosine in DNA promotes genome stability

     Brooke A Conti, Leo Novikov ... Mariano Oppikofer

     Single-cell imaging and mass spectrometry data reveal adenosine in the DNA of mammalian cells can be methylated by METTL3 to form N6-methyladenosine in response to DNA damage.
180. 1. Neuroscience

     Effort drives saccade selection

     Damian Koevoet, Laura Van Zantwijk ... Christoph Strauch

     Humans optimize effort expenditure even at the level of eye movements by choosing affordable eye movements over costly alternatives and adjusting gaze behavior based on task demands.
181. 1. Neuroscience

     A novel method (RIM-Deep) for enhancing imaging depth and resolution stability of deep cleared tissue in inverted confocal microscopy

     Yisi Liu, Pu Wang ... Hongwei Zhou

     RIM-Deep enhances imaging depth in confocal microscopy enabling high-quality visualization of large cleared samples across various fields with minimal cost.
182. 1. Neuroscience

     Synaptic cell adhesion molecule Cdh6 identifies a class of sensory neurons with novel functions in colonic motility

     Julieta Gomez-Frittelli, Gabrielle Frederique Devienne ... Julia A Kaltschmidt

     Cadherin-6 (Cdh6) marks gut-intrinsic sensory neurons, and optogenetic activation of Cdh6+ sensory neurons is sufficient to evoke colonic motor complexes.
183. 1. Immunology and Inflammation
     2. Structural Biology and Molecular Biophysics

     Ab initio prediction of specific phospholipid complexes and membrane association of HIV-1 MPER antibodies by multi-scale simulations

     Colleen A Maillie, Kiana Golden ... Marco Mravic

     Molecular dynamics simulations reveal that lipid binding at antibody loops and framework regions stabilizes membrane interactions, providing molecular details for neutralization mechanisms and strategies for HIV-1 vaccine development.
184. 1. Biochemistry and Chemical Biology
     2. Immunology and Inflammation

     SAM transmethylation pathway and adenosine recycling to ATP are essential for systemic regulation and immune response

     Pavla Nedbalová, Nikola Kaislerova ... Tomáš Doležal

     Adenosine produced by transmethylation may act as a sensor of the balance between cell activity and nutrient supply.
185. 1. Microbiology and Infectious Disease

     FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis

     McKenna Harpring, Junghoon Lee ... John V Cox

     The novel divisome complex of Chlamydia trachomatis contains elements of the divisome and elongasome from other bacteria, and it requires the chromosomal translocase, FtsK, for its assembly.
186. 1. Immunology and Inflammation
     2. Structural Biology and Molecular Biophysics

     Load-based divergence in the dynamic allostery of two TCRs recognizing the same pMHC

     Ana Cristina Chang-Gonzalez, Aoi Akitsu ... Wonmuk Hwang

     Recognition of antigenic ligand by T-cell receptors is a nuanced process where mechanical force can be leveraged to diversify functional outcomes of different T-cells for the same ligand.
187. 1. Plant Biology

     The rhizobial effector NopT targets Nod factor receptors to regulate symbiosis in Lotus japonicus

     Hanbin Bao, Yanan Wang ... Yangrong Cao

     The rhizobial effector NopT and two Nod factor receptors of Lotus japonicus, NFR1 and NFR5, mutually interact to precisely regulate rhizobial symbiosis.
188. 1. Neuroscience
     2. Physics of Living Systems

     Annihilation of action potentials induces electrical coupling between neurons

     Moritz Schloetter, Georg U Maret, Christoph J Kleineidam

     Action potentials of neurons expel a charge when they reach the axon terminal, and classical electrodynamics predict the resulting electric fields and its influence to other neurons by ephaptic coupling.
189. 1. Cell Biology

     Stratification of enterochromaffin cells by single-cell expression analysis

     Yan Song, Linda J Fothergill ... Gene W Yeo

     Molecularly defined serotonin (enterochromaffin) cells of the gut lining were distinguished by single-cell expression analysis, in situ hybridization, and immunohistochemistry into 14 topographically organized clusters, with features implying that they are functionally distinct classes of serotonin cells.
190. 1. Developmental Biology

     Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation

     Mehmet Mahsum Kaplan, Erika Hudacova ... Ondrej Machon

     Meis2 transcription factor instructs the mesenchyme to initiate whisker formation in the epithelium.
191. 1. Ecology
     2. Evolutionary Biology

     Reconstructing the phylogeny and evolutionary history of freshwater fishes (Nemacheilidae) across Eurasia since early Eocene

     Vendula Bohlen Šlechtová, Tomáš Dvořák ... Joerg Bohlen

     The first comprehensive reconstruction of the evolutionary history of freshwater animals, on example of family Nemacheilidae, across Eurasia with the identification of the main tectonic, geological, and climatic factors influencing it has been described.
192. 1. Microbiology and Infectious Disease

     Linalool combats Saprolegnia parasitica infections through direct killing of microbes and modulation of host immune system

     Tao Tang, Weiming Zhong ... Zhipeng Gao

     Linalool demonstrates dual antimicrobial and immunomodulatory actions against Saprolegnia parasitica.
193. 1. Cell Biology

     Small-molecule activation of TFEB alleviates Niemann–Pick disease type C via promoting lysosomal exocytosis and biogenesis

     Kaili Du, Hongyu Chen ... Dan Li

     Small-molecule agonists of transcription factor EB alleviate Niemann–Pick disease type C via promoting lysosomal function.
194. 1. Immunology and Inflammation

     CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells

     Xiaoyu Meng, Yezhang Zhu ... Lie Wang

     CXXC1 is a pivotal epigenetic regulator that ensures T_reg homeostasis and suppressive function by interacting with FOXP3 to modulate H3K4me3 deposition at key signature genes.
195. 1. Developmental Biology
     2. Neuroscience

     A library of lineage-specific driver lines connects developing neuronal circuits to behavior in the Drosophila Ventral Nerve Cord

     Jelly HM Soffers, Erin Beck ... Haluk Lacin
196. 1. Chromosomes and Gene Expression
     2. Immunology and Inflammation

     Broad-spectrum immune suppression encoded in self-amplifying RNA enables non-cytotoxic, non-immunostimulatory, externally controllable transgene expression

     Tony KY Lim, Anne Ritoux ... Ewan St John Smith
197. 1. Developmental Biology

     A-to-I RNA editing of CYP18A1 mediates transgenerational wing dimorphism in aphids

     Bin Zhu, Rui Wei ... Pei Liang

     Binding of miR-3036-5p to CYP18A1 can be prevented by an A-to-I RNA editing, thus elevating CYP18A1 expression, decreasing 20E titer, and finally regulating the wing dimorphism of offspring in aphid.
198. 1. Neuroscience

     Visual routines for detecting causal interactions are tuned to motion direction

     Sven Ohl, Martin Rolfs

     Visual adaptation in humans revealed that the perception of causality was tuned to the direction of motion while the adaptation transferred across different motion speeds and colors.
199. 1. Neuroscience

     Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism models

     Ulrike Pech, Jasper Janssens ... Patrik Verstreken

     A new Drosophila collection of Parkinsonism models reveals early synaptic cholinergic projection neuron dysfunction, linking synaptic failure to later dopaminergic decline, highlighting a role for cholinergic modulation in dopaminergic neuron health.
200. 1. Neuroscience

     Serotonin modulates infraslow oscillation in the dentate gyrus during non-REM sleep

     Gergely F Turi, Sasa Teng ... Yueqing Peng

     Rhythmic release of serotonin organizes infraslow oscillatory activity of the dentate gyrus during sleep.
201. 1. Stem Cells and Regenerative Medicine

     Simultaneous cyclin D1 overexpression and p27^kip1 knockdown enable robust Müller glia cell cycle reactivation in uninjured mouse retina

     Zhifei Wu, Baoshan Liao ... Wenjun Xiong

     A novel strategy is described for stimulating a large number of Müller glia to proliferate in mice by manipulating cell cycle components without a damage stimulus.
202. 1. Developmental Biology

     Neuroprotective role of Hippo signaling by microtubule stability control in Caenorhabditis elegans

     Hanee Lee, Junsu Kang ... Junho Lee

     The Hippo pathway maintains the integrity of differentiated neurons by modulating microtubule stability, and reducing microtubule stability of fully developed neurons offers a potential strategy to delay neuronal aging.
203. 1. Medicine

     Pyrotinib after trastuzumab-based adjuvant therapy in patients with HER2-positive breast cancer (PERSIST): A multicenter phase II trial

     Feilin Cao, Zhaosheng Ma ... Shifen Huang

     Kaplan–Meier curves showed that extended adjuvant pyrotinib administered after trastuzumab-based adjuvant therapy provides a promising survival benefit in patients with high-risk HER2-positive breast cancer.
204. 1. Genetics and Genomics
     2. Microbiology and Infectious Disease

     Control of pili synthesis and putrescine homeostasis in Escherichia coli

     Iti Mehta, Jacob B Hogins ... Larry Reitzer

     Putrescine has a homeostatic network that controls pili synthesis and energy metabolism.
205. 1. Computational and Systems Biology

     A network regularized linear model to infer spatial expression pattern for single cell

     Chaohao Gu, Hu Chen, Zhandong Liu
206. 1. Neuroscience

     Longitudinal assessment of DREADD expression and efficacy in the monkey brain

     Yuji Nagai, Yukiko Hori ... Takafumi Minamimoto
207. 1. Cell Biology

     The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods

     Ziv Yaniv, Ifeanyichukwu U Anidi ... Andrea J Radtke
208. 1. Developmental Biology
     2. Evolutionary Biology

     Molecular dynamics of the matrisome across sea anemone life history

     B Gideon Bergheim, Alison G Cole ... Suat Özbek
209. 1. Chromosomes and Gene Expression
     2. Microbiology and Infectious Disease

     Single-cell RNA-seq reveals trans-sialidase-like superfamily gene expression heterogeneity in Trypanosoma cruzi populations

     Lucas Inchausti, Lucía Bilbao ... Pablo Smircich
210. 1. Cell Biology
     2. Developmental Biology

     Zinc is a Key Regulator of the Sperm-Specific K⁺ Channel (Slo3) Function

     Rizki Tsari Andriani, Tanadet Pipatpolkai ... Takafumi Kawai
211. 1. Microbiology and Infectious Disease
     2. Epidemiology and Global Health

     Estimation of Rotavirus Vaccine Effectiveness Based on Whole Genome Sequences

     Jiye Kwon, Jose Jaimes ... Virginia E Pitzer
212. 1. Developmental Biology

     MMP21 behaves as a fluid flow transported morphogen to impart laterality during development

     Tim Ott, Amelie Brugger ... Axel Schweickert
213. 1. Structural Biology and Molecular Biophysics

     Dimerization and dynamics of angiotensin-I converting enzyme revealed by cryoEM and MD simulations

     Jordan M Mancl, Xiaoyang Wu ... Wei-Jen Tang
214. 1. Microbiology and Infectious Disease

     Heat Stress Induced Bacterial Tolerance against Phage Facilitates the Evolution of Resistance

     Fan Zhang, Hao-Ze Chen ... Liu Jiafeng
215. 1. Genetics and Genomics
     2. Evolutionary Biology

     Lifestyles shape genome size and gene content in fungal pathogens

     Anna Fijarczyk, Pauline Hessenauer ... Christian R Landry
216. 1. Neuroscience

     Viral-mediated Oct4 overexpression and inhibition of Notch signaling synergistically induce neurogenic competence in mammalian Müller glia

     Nguyet Le, Sherine Awad ... Seth Blackshaw
217. 1. Evolutionary Biology

     A direct experimental test of Ohno’s hypothesis

     Ljiljana Mihajlovic, Bharat Ravi Iyengar ... Yolanda Schaerli

     Experimental evolution of a duplicated gene increases mutational robustness relative to a single-copy gene but does not accelerate phenotypic evolution.
218. 1. Computational and Systems Biology

     Redox regulation and dynamic control of brain-selective kinases BRSK1/2 in the AMPK family through cysteine-based mechanisms

     George N Bendzunas, Dominic P Byrne ... Natarajan Kannan

     Characterization of redox-active cysteines illuminates the understudied regulatory mechanisms of brain-selective kinases.
219. 1. Neuroscience

     Rab10 regulates neuropeptide release by maintaining Ca²⁺ homeostasis and protein synthesis

     Jian Dong, Mian Chen ... Matthijs Verhage

     Rab10 deficiency unexpectedly inhibits neuropeptide secretion through two distinct effects on the endoplasmic reticulum (ER), disrupting ER-regulated intracellular Ca²⁺ dynamics and impairing protein synthesis.
220. 1. Developmental Biology

     Knockout of cyclin-dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice

     Alexandra V Bruter, Ekaterina A Varlamova ... Victor V Tatarskiy

     Knockout of CDK8/19, but not its inhibition, impairs spermatogenesis by reducing steroid production in Leydig cells.
221. 1. Neuroscience

     The NIH BRAIN Initiative’s Experiment in Team Research

     Farah Bader, Karen K David ... On behalf of the NIH BRAIN Initiative Integrative and Quantitative Neuroscience Team
222. 1. Neuroscience

     Activity-Dependent Changes in Ion Channel Voltage-Dependence Influence the Activity Patterns Targeted by Neurons

     Yugarshi Mondal, Ronald L Calabrese, Eve Marder
223. 1. Neuroscience

     The NIH BRAIN Initiative’s Impacts in Systems and Computational Neuroscience, 2014-2023

     Farah Bader, Clayton Bingham ... On behalf of the NIH BRAIN Initiative Integrative and Quantitative Neuroscience Team
224. 1. Genetics and Genomics

     Molecular Requirements for C. elegans Transgenerational Epigenetic Inheritance of Pathogen Avoidance

     Rachel Kaletsky, Rebecca Moore ... Coleen T Murphy
225. 1. Immunology and Inflammation
     2. Microbiology and Infectious Disease

     Nora virus proliferates in dividing intestinal stem cells and sensitizes flies to intestinal infection and oxidative stress

     Adrien Franchet, Samantha Haller ... Dominique Ferrandon
226. 1. Biochemistry and Chemical Biology

     Enzymatic protein fusions with 100% product yield

     Adrian CD Fuchs

     This enzymatic method enables highly specific and complete 1:1 protein conjugations with small molecules, solid supports, and other proteins, offering broad applicability across diverse biochemical and biotechnological applications.
227. 1. Physics of Living Systems

     Modularity of the segmentation clock and morphogenesis

     James E Hammond, Ruth E Baker, Berta Verd

     Computational modelling suggests that the segmentation clock and the morphogenesis of the pre-somitic mesoderm exhibit developmental modularity, enabling the independent evolution of these processes and possibly contributing to the high evolvability of vertebrate segment number.
228. 1. Genetics and Genomics

     UBR-1 deficiency leads to ivermectin resistance in Caenorhabditis elegans

     Yi Li, Long Gong ... Shangbang Gao

     Behavioral and functional analyses revealed that UBR-1 deficiency in Caenorhabditis elegans disrupts glutamate signaling and confers ivermectin resistance.
229. 1. Neuroscience

     Infralimbic parvalbumin neural activity facilitates cued threat avoidance

     Yi-Yun Ho, Qiuwei Yang ... Melissa R Warden

     Infralimbic inhibitory parvalbumin neurons play a counterintuitive role in supporting flexible behavior in the face of threat.
230. 1. Plant Biology
     2. Structural Biology and Molecular Biophysics

     Structure of the Calvin-Benson-Bassham sedoheptulose-1,7-bisphosphatase from the model microalga Chlamydomonas reinhardtii

     Théo Le Moigne, Martina Santoni ... Julien Henri

     Crystallography, molecular dynamics simulations, and biochemical analyses of Calvin-Benson-Bassham sedoheptulose-1,7-bisphosphatase (SBPase) from the microalga Chlamydomonas reinhardtii identify a redox module that controls oligomeric states for the activation of the enzyme.
231. 1. Neuroscience

     Peripheral opioid receptor antagonism alleviates fentanyl-induced cardiorespiratory depression and is devoid of aversive behavior

     Brian C Ruyle, Sarah Masud ... Jose A Morón

     The peripherally restricted opioid receptor antagonist naloxone methiodide reverses fentanyl-induced respiratory depression without causing aversive behaviors that are observed after naloxone.