December 2025

Cover articles

    1. Cell Biology

    The power of pyruvate

    Ashish G Toshniwal, Geanette Lam ... Jared Rutter
    1. Developmental Biology

    Early influences on cell fate

    Adiyant Lamba, Meng Zhu ... Magdalena Zernicka-Goetz
    1. Developmental Biology

    Eed and craniofacial development

    Tim Casey-Clyde, S John Liu ... David R Raleigh
    1. Cell Biology

    Phosphate buffering

    Samuel Bru, Lydie Michaillat Mayer ... Andreas Mayer

Highlights controls:

Research articles

    1. Developmental Biology
    2. Neuroscience

    From Syllables to Words: EEG Evidence of Different Age Trajectories in Speech Tracking and Statistical Learning in Infants at High and Low Likelihood for Autism

    Michel Godel, Ana Fló ... Marie Schaer
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Dynamic updating of spatial working memory across eye movements: a computational investigation of transsaccadic integration

    Sijia Zhao, Thomas Parr ... Masud Husain
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    How individual vigor shapes human-human physical interaction

    Dorian Verdel, Bastien Berret, Etienne Burdet
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Neisseria gonorrhoeae LIN codes provide a robust, multi-resolution lineage nomenclature

    Anastasia Unitt, Made A Krisna ... Odile B Harrison
    LIN code offers a reliable framework for classifying gonococcal population structure, enabling straightforward exploration of isolate relationships, from broad superlineage divisions to minute single allele differences.
    1. Neuroscience

    Coordinated spinal locomotor network dynamics emerge from cell-type-specific connectivity patterns

    F David Wandler, Benjamin K Lemberger ... James M Murray
    A hierarchy of models guided by experimental results from zebrafish shows that coordinated, variable-speed locomotion can emerge from network-level interactions among interneuron populations with structured spatial connectivity.
    1. Physics of Living Systems

    The evolution of interdisciplinarity and internationalization in scientific journals

    Huaxia Zhou, Luis A Nunes Amaral
    Analysis of ~50 million articles published in ~50 thousand journals demonstrates that journals have become more diverse in terms of both interdisciplinarity and internationalization but with some disciplinary differences.
    1. Neuroscience

    Decision-making components and times revealed by the single-trial electroencephalogram

    Gabriel Weindel, Jelmer P Borst, Leendert van Maanen
    By modeling the single-trial electroencephalogram of participants performing perceptual decisions, and building on predictions from two century-old psychological laws, we estimate the times of information processing steps contained in decision-making.
    1. Cell Biology

    JAK-STAT pathway activation compromises nephrocyte function in a Drosophila high-fat diet model of chronic kidney disease

    Yunpo Zhao, Jianli Duan ... Zhe Han
    A conserved signaling axis linking Drosophila adipose tissue to nephrocyte function reveals how obesity can drive kidney dysfunction and points to new opportunities for therapeutic intervention.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Hybridization breaks species barriers in long-term coevolution of a cyanobacterial population

    Gabriel Birzu, Harihara Subrahmaniam Muralidharan ... Devaki Bhaya
    Analysis of hundreds of single-cell genomes from Yellowstone National Park shows bacterial species are less cohesive than previously thought.
    1. Neuroscience

    Retrosplenial cortex enables context-dependent goal-directed sensorimotor transformation

    Pol Bech, Robin F Dard ... Carl CH Petersen
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Prolonged oscillating kisspeptin neuron activity underlies the preovulatory luteinizing hormone surge in mice

    Ziyue Zhou, Cheng-Yu Huang, Allan E Herbison
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    Evolution of a fuzzy ribonucleoprotein complex in viral assembly

    Huaying Zhao, Tiansheng Li ... Peter Schuck
    The stability of ribonucleoprotein complexes of SARS-CoV-2 nucleocapsid proteins differs among variants of concern and the viral evolution of nucleocapsid protein can be understood in the framework of fuzzy complexes.
    1. Immunology and Inflammation

    Epidermal resident memory T cell fitness requires antigen encounter in the skin

    Eric S Weiss, Toshiro Hirai ... Daniel H Kaplan
    Antigen-driven TCR signaling in the epidermis during CD8+ TRM differentiation results in a lower TGFβ requirement for persistence and increased proliferative capacity that together enhance epidermal TRM fitness.
    1. Genetics and Genomics
    2. Medicine

    Translational reading frame determines the pathogenicity of C-terminal frameshift deletions in MeCP2: an alternative therapeutic approach

    Jacky Guy, Elena Hein ... Adrian Bird
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    A differentiable model for optimizing the genetic drivers of synaptogenesis

    Tommaso Boccato, Matteo Ferrante, Nicola Toschi
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Ecology

    The urban tree of life: quantifying relationships between body size and urban tolerance for more than 30,000 plant and animal species

    Corey T Callaghan, Diana E Bowler ... Thomas Merckx
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Continuous developmental changes in word recognition support language learning across early childhood

    Michael C Frank, Virginia A Marchman ... Martin Zettersten
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Spatial learning in multi-scale environments: Roles of hippocampus, orbitofrontal cortex, and retrosplenial cortex

    Yidan Qiu, Senning Zheng ... Ruiwang Huang
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    DANCE provides an open-source and low-cost approach to quantify aggression and courtship in Drosophila

    R Sai Prathap Yadav, Paulami Dey ... Pavan Agrawal
    A low-cost, portable platform combining machine learning–based classifiers enables accurate quantification of Drosophila aggression and courtship, expanding access to behavioral neuroscience in resource-limited settings.
    1. Cancer Biology

    Therapeutic benefits of maintaining CDK4/6 inhibitors and incorporating CDK2 inhibitors beyond progression in breast cancer

    Jessica Armand, Sungsoo Kim ... Hee Won Yang
    Maintaining CDK4/6 inhibition constrains drug‑resistant breast tumors by delaying cell‑cycle entry, and adding CDK2 inhibition further deepens control to guide post‑progression therapy.
    1. Medicine
    2. Neuroscience

    Dual-modal metabolic analysis reveals hypothermia-reversible uncoupling of oxidative phosphorylation in neonatal brain hypoxia-ischemia

    Naidi Sun, Yu-Yo Sun ... Song Hu
    Post‑HI oxidative‑phosphorylation uncoupling drives secondary energy failure in neonatal brains, and preventing the post‑HI CMRO2 surge is a key mechanism underlying hypothermia’s neuroprotection.
    1. Microbiology and Infectious Disease

    A system for functional studies of the major virulence factor of malaria parasites

    Jakob Cronshagen, Johannes Allweier ... Tobias Spielmann
    The generation of malaria parasites expressing only one of the multiple variants of their most important virulence factor enables the study of different aspects of this virulence factor.
    1. Cancer Biology

    Sex differences in bile acid homeostasis and excretion underlie the disparity in liver cancer incidence between males and females

    Megan E Patton, Sherwin Kelekar ... Sayeepriyadarshini Anakk
    Transcriptomic and metabolite analysis reveal sex differences in circulation and excretion of bile acids in a mouse- model of hepatocellular carcinoma (HCC), and the identified female-specific gene profile correlates with better HCC survival.
    1. Computational and Systems Biology
    2. Neuroscience

    Morphogenesis and morphometry of brain folding patterns across species

    Sifan Yin, Chunzi Liu ... L Mahadevan
    The morphogenesis of brain folding patterns across species is studied by physical gel models, simulations, and a quantitative morphometric analysis.
    1. Developmental Biology
    2. Neuroscience

    Biophysical basis for brain folding and misfolding patterns in ferrets and humans

    Gary PT Choi, Chunzi Liu ... L Mahadevan
    Physical gel models and numerical simulations have been created to study ferret brain gyrification and cortical malformations, while comparisons with human phenotypes aim to link genetics and brain morphogenesis.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Glycosylated IgG antibodies contribute to the recovery of haemorrhagic fever with renal syndrome patients

    Chuansong Quan, Lu Wang ... Haifeng Hou
    Increased fucosylated IgG and reduced bisecting GlcNAc during HTNV convalescence may promote rapid recovery from hemorrhagic fever with renal syndrome.
    Version of Record
    Short Report
    • Valuable
    • Solid
    1. Developmental Biology

    ANKEF1 is a key axonemal component essential for murine sperm motility and male fertility

    Shuntai Yu, Guoliang Yin ... Fengchao Wang
    ANKEF1 stabilizes the sperm axoneme by reinforcing the nexin–dynein regulatory complex, revealing a mechanical buffering mechanism essential for flagellar integrity and male fertility.
    1. Immunology and Inflammation

    Class A scavenger receptor MARCO negatively regulates Ace expression and aldosterone production

    Conan JO O'Brien, Giorgio Ratti ... Ana I Domingos
    Loss of class A scavenger receptor MARCO results in elevated lung Ace expression and elevated plasma aldosterone concentrations in male mice, demonstrating an endocrine function of this immune scavenger receptor.
    1. Neuroscience

    Projection-specific integration of convergent thalamic and retrosplenial signals in the presubicular head direction cortex

    Louis Richevaux, Dongkyun Lim ... Desdemona Fricker
    Nonlinear dendritic integration in single presubicular neurons provides a mechanism for combining vestibular-based head-direction signals and visual landmark signals to anchor the brain’s internal compass.
    1. Chromosomes and Gene Expression

    Structural characterisation of chromatin remodelling intermediates supports linker DNA dependent product inhibition as a mechanism for nucleosome spacing

    Amanda L Hughes, Ramasubramanian Sundaramoorthy, Tom Owen-Hughes
    1. Structural Biology and Molecular Biophysics

    Nucleosome wrapping energy in CpG islands and the role of epigenetic base modifications

    Rasa Giniūnaitė, Rahul Sharma ... Daiva Petkevičiūtė-Gerlach
    CpG density and epigenetic modification state modulate DNA mechanics, altering its intrinsic compatibility with nucleosome formation.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Phospholipid scramblase 1 (PLSCR1) regulates interferon-lambda receptor 1 (IFN-λR1) and IFN-λ signaling in influenza A virus (IAV) infection

    Alina Xiaoyu Yang, Lisa Ramos-Rodriguez ... Yang Zhou
    PLSCR1 is an IFN-λ-stimulated gene that enhances IFN-λR1 expression at both transcriptional and protein level, highlighting the potential for targeting PLSCR1 pathways in anti-influenza therapy.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Enteropathogenic E. coli-mediated Fast and Coordinated Ca²⁺ responses regulate NF-κB activation

    Fangrui Guo, Roberto Ornelas Guevara ... Guy Tran Van Nhieu
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Daily life fluctuations in affect predict within-person changes in a real-world measure of cognitive processing speed

    Sol Fittipaldi, Vanessa Teckentrup ... Claire M Gillan
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Medicine

    Are peer reviewers influenced by their work being cited?

    Adrian Barnett
    Some requests by reviewers to cite their own publications are coercive and can unnecessarily delay indexation and publication.
    1. Immunology and Inflammation

    Trained Immunity: RoadMap for drug discovery and development

    Jelmer H van Puffelen, Callum Campbell ... Pauline T Lukey
    Combining scientific rigour and drug development experience to map out a route to novel treatments for Trained Immunity-dependent diseases.
    1. Developmental Biology

    Mac-/Lactosylceramide regulates intestinal homeostasis and secretory cell fate commitment by facilitating Notch signaling

    Kebei Tang, Xuewen Li ... Rongwen Xi
    An evolutionarily conserved glycosphingolipid metabolite ensures precise stem cell fate decisions by modulating Delta-Notch signaling dynamics.
    1. Cell Biology

    A single-cell atlas of the testicular interstitium defines Leydig progenitor networks sustaining Leydig cell homeostasis across the lifespan

    Xiaojia Huang, Kai Xiao Xia ... Wei Zhao
    1. Computational and Systems Biology
    2. Neuroscience

    Predictive modeling of hematoma expansion from non-contrast computed tomography in spontaneous intracerebral hemorrhage patients

    Natasha Ironside, Kareem El Naamani ... VISTA-ICH
    In spontaneous intracerebral hemorrhage patients, features of hematoma expansion can be visualized and predicted from non-contrast computed tomography using transport-based morphometry.
    1. Evolutionary Biology

    Cryptovaranoides is not a squamate

    Michael W Caldwell, Chase D Brownstein ... Tiago R Simões
    Claims for a Triassic-aged crown lizard merit detailed reanalysis, the results of which find that Cryptovaranoides is not only not a lizard, but is a more distantly related diapsid reptile.
    1. Neuroscience

    Regional heterogeneities of oligodendrocytes underlie biased Ranvier node spacing along single axons in sound localization circuit

    Ryo Egawa, Kota Hiraga ... Hiroshi Kuba
    Oligodendrocytes with different morphological features work together to support precise binaural integration for sound localization in the chick brainstem auditory circuit.
    1. Neuroscience

    DendroTweaks, an interactive approach for unraveling dendritic dynamics

    Roman Makarov, Spyridon Chavlis, Panayiota Poirazi
    An interactive toolbox for standardizing, validating, simulating, reducing, and exploring detailed biophysical models that can be used to reveal how morpho-electric properties map to dendritic and neuronal outputs.
    1. Microbiology and Infectious Disease

    Functional genomics reveals strain-specific genetic requirements conferring hypoxic growth in Mycobacterium intracellulare

    Yoshitaka Tateishi, Yuriko Ozeki ... Sohkichi Matsumoto
    Transposon sequencing analyses reveal that among genomically diverse clinical Mycobacterium intracellulare strains and the type strain, 131 genes were comprehensively identified as universal essential genes suggesting promising drug targets.
    1. Neuroscience

    Vascular endothelial-specific loss of TGF-beta signaling as a model for choroidal neovascularization and central nervous system vascular inflammation

    Yanshu Wang, Amir Rattner ... Jeremy Nathans
    In mice, loss of TGF-beta signaling specifically in vascular endothelial cells leads to retinal hypovascularization, choroidal neovascularization, a pro-inflammatory state within brain and retinal endothelial cells, and an influx of diverse inflammatory cells into the retina.
    1. Developmental Biology
    2. Genetics and Genomics

    Computer prediction and genetic analysis identifies retinoic acid modulation as a driver of conserved longevity pathways in genetically diverse Caenorhabditis nematodes

    Stephen A Banse, Christine A Sedore ... Patrick C Phillips
    Computer screening identifies that the widely prescribed vitamin A derivative all-trans retinoic acid increases both longevity and late-life health in a powerful genetic model system.
    1. Medicine
    2. Neuroscience

    GPR30 in spinal cholecystokinin-positive neurons modulates neuropathic pain

    Qing Chen, Hui Wu ... Xinzhong Chen
    A combination of viral strategies, electrophysiological methods, and behavioral tests reveals how GPR30 in spinal CCK+ neurons modulates neuropathic pain.
    1. Genetics and Genomics

    Microbiota impact Drosophila ageing via Acetobacter, Tachykinin, and TkR99D

    Diana Marcu, David R Sannino ... Adam J Dobson
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology

    Sex-biased expression of enteroendocrine cell-derived hormones contributes to higher fat storage in Drosophila females

    Puja Biswas, Elizabeth J Rideout
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    1. Neuroscience

    Synergistic MAPT mutations as a platform to uncover modifiers of tau pathogenesis

    Miles R Bryan, Michael F Almeida ... Todd J Cohen
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    • Solid
    1. Neuroscience

    Decoding Spine Nanostructure in Mental Disorders Reveals a Schizophrenia-Linked Role for Ecrg4

    Yutaro Kashiwagi, Qingrui Liu ... Shigeo Okabe
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Emergence of Functional Heart-Brain Circuits in a Vertebrate

    Luis Hernandez-Nunez, Joana Avrami ... Mark C Fishman
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Neuroscience

    Intersection of transient cell states with stable cell types in hippocampus

    Jack A Olmstead, Lauren E King, Brenda L Bloodgood
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    Structural basis for collagen recognition by the Streptococcus pyogenes M3 protein and its involvement in biofilm

    Marta Wojnowska, Takeaki Wajima ... Ulrich Schwarz-Linek
    The T-shaped fold of an abundant surface protein ties streptococci to collagens, an interaction that enhances biofilm such as often found in invasive infections.
    1. Neuroscience

    Cerebellar climbing fibers impact experience-dependent plasticity in the mouse primary somatosensory cortex

    Abby Silbaugh, Kevin P Koster, Christian Hansel
    Optogenetic climbing fiber activation regulates experience-dependent plasticity in the primary somatosensory cortex of mice, suggesting a role of the olivo-cerebellum in instructive signaling across brain regions.
    1. Neuroscience

    Afadin-deficient mouse retinas exhibit severe neuronal lamination defects but preserve visual functions

    Akiko Ueno, Konan Sakuta ... Chieko Koike
    Afadin-deficient mice, whose outer-retinal lamination and photoreceptor synapses are severely disrupted, nonetheless partially retain retinal neural circuits and visual function.
    1. Computational and Systems Biology
    2. Neuroscience

    Human EEG and artificial neural networks reveal disentangled representations and processing timelines of object real-world size and depth in natural images

    Zitong Lu, Julie Golomb
    Neural and computational evidence reveals that real-world size is a temporally late, semantically grounded, and hierarchically stable dimension of object representation in both human brains and artificial neural networks.
    1. Neuroscience

    Cone bipolar cell synapses generate transient versus sustained signals in parallel ON pathways of the mouse retina

    Sidney P Kuo, Wan-Qing Yu ... Fred Rieke
    Differences in kinetics of retinal output signals originate at least in part from differences in synaptic output from distinct bipolar cell types.
    1. Neuroscience

    Non-decision time-informed collapsing threshold diffusion model: A joint modeling framework with identifiable time-dependent parameters

    Amir Hosein Hadian Rasanan, Lukas Schumacher ... Jörg Rieskamp
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Ecology

    Exogenous myristate fuels the growth of symbiotic arbuscular mycorrhizal fungi but disrupts their carbon-phosphorus exchange with host plants

    Hanwen Chen, Tian Xiong ... Yutao Wang
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Developmental Biology
    2. Neuroscience

    Voltage imaging reveals the emergence of population activity in the spinal cord

    Asuka Shiraishi, Ayane Hayashi ... Sachiko Tsuda
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Maximized field-of-view deep-brain calcium imaging through gradient-index lenses

    Chenmao Wang, Zongyue Cheng ... Meng Cui
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    Brain Perfusion Imaging of a Large Population: Arterial Spin Labelling MRI in UK Biobank

    Thomas W Okell, Xinyi Xu ... Michael A Chappell
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Microbiology and Infectious Disease

    Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis

    Kamal L Nahas, Viv Connor ... Colin M Crump
    Deletion of specific herpes simplex virus proteins prevents the progression of virus assembly at discrete stages, allowing detailed 3D imaging of rare events in the morphogenesis of virus particles.
    1. Evolutionary Biology
    2. Genetics and Genomics

    G-protein-coupled receptor diversity and evolution in the closest living relatives of metazoa

    Alain Garcia De Las Bayonas, Nicole King
    Up-to-date computational pipeline characterizes G-protein-coupled receptor (GPCR) repertoires in the closest relatives of metazoans, providing a framework to investigate the evolutionary origins and ancestral functions of key metazoan signaling pathways.
    1. Biochemistry and Chemical Biology

    A green lifetime biosensor for calcium that remains bright over its full dynamic range

    Franka H van der Linden, Stephen C Thornquist ... Joachim Goedhart
    Directed evolution was used to engineer a green fluorescent biosensor for quantitative calcium imaging that remains fluorescent, yet shows a large change in lifetime in cells.
    1. Neuroscience

    PRRT2 as an auxiliary regulator of Nav channel slow inactivation

    Bin Lu, Qi-Wu Xu ... Zhi-Qi Xiong
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Structural Biology and Molecular Biophysics

    Structural mechanisms of pump assembly and drug transport in the AcrAB-TolC efflux system

    Xiaofei Ge, Zhiwei Gu, Jiawei Wang
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    • Incomplete
    1. Evolutionary Biology

    Brawn before bite in endemic Asian mammals after the end-Cretaceous extinction

    Z Jack Tseng, Qian Li, Suyin Ting
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Cell Biology

    Testosterone-induced metabolic changes in seminal vesicle epithelium modify seminal plasma components with potential to improve sperm motility

    Takahiro Yamanaka, Zimo Xiao ... Masayuki Shimada
    Testosterone promotes the conversion of glucose to fatty acids by increasing the expression of ACLY in seminal vesicle epithelial cells, thereby regulating the seminal plasma components.
    1. Structural Biology and Molecular Biophysics

    Insights into substrate binding and utilization by hyaluronan synthase

    Zachery Stephens, Julia Karasinska, Jochen Zimmer
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Cell Biology

    CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

    Maria Giovanna De Leo, Andreas Mayer
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    • Incomplete
    1. Developmental Biology

    Extracellular adenosine deamination primes tip organizer development in Dictyostelium

    Pavani Hathi, Baskar Ramamurthy
    Extracellular adenosine deamination by ADGF generates ammonia that directs tip organizer development, revealing a conserved metabolic mechanism linking nucleotide turnover to developmental regulation.
    1. Developmental Biology

    Endothelial Slit2 guides the Robo1-positive sympathetic innervation during heart development

    Juanjuan Zhao, Susann Bruche ... Mathilda Mommersteeg
    Loss of endothelial Slit2 removes the chemo-attractive signal needed for Robo1-positive sympathetic axons to follow coronary vessels, resulting in reduced ventricular innervation.
    Version of Record
    Short Report
    • Important
    • Convincing
    1. Neuroscience

    On CA1 ripple oscillations in rats and the reassessment of asynchronicity evidence

    Robson Scheffer-Teixeira, Adriano BL Tort
    Ripples maintain time-locked occurrence across the septo-temporal axis and hemispheres while showing local phase coupling, revealing a dual mode of synchrony in CA1 network dynamics.
    1. Plant Biology

    An increase of NPY1 expression leads to inhibitory phosphorylation of PIN-FORMED (PIN) proteins and suppression of pinoid (pid) null mutants

    Michael Mudgett, Zhouxin Shen ... Yunde Zhao
    A cause and impact of PIN-FORMED protein phosphorylation has been revealed.
    1. Cancer Biology

    Multi-gradient permutation survival analysis identifies mitosis and immune signatures steadily associated with cancer patient prognosis

    Xinlei Cai, Yi Ye ... Hongbin Ji
    Genes steadily associated with prognosis define conserved mitotic and immune programs that robustly predict cancer patient survival and therapeutic resistance across tumor types.
    1. Microbiology and Infectious Disease

    An in vitro human vessel model to study Neisseria meningitidis colonization and vascular damages

    Léa Pinon, Melanie Chabaud ... Guilllaume Dumenil
    A vessel on a chip microfluidic device recapitulates the main features of meningococcal vascular colonization, providing high time and space microscopy resolution of the infection process in 3D.
    1. Microbiology and Infectious Disease

    Niche exclusion of a lung pathogen in mice with designed probiotic communities

    Kelsey E Hern, Ashlee M Phillips ... Adam P Arkin
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Neural activity profiles reveal overlapping, intermingled subpopulations spanning area borders in mouse sensorimotor cortex

    Sohrab Salimian, Harrison A Grier, Matthew T Kaufman
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Neuroscience

    High-Fidelity Neural Speech Reconstruction through an Efficient Acoustic-Linguistic Dual-Pathway Framework

    Jiawei Li, Chunxu Guo ... Yuanning Li
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Evolutionary Biology
    2. Genetics and Genomics

    Lifestyles shape genome size and gene content in fungal pathogens

    Anna Fijarczyk, Pauline Hessenauer ... Christian R Landry
    A genomic analysis shows that the evolution of genome size and gene content among fungal pathogens in the class Sordariomycetes varies depending on their interactions with hosts and vectors.
    1. Cell Biology

    The fate of pyruvate dictates cell growth by modulating cellular redox potential

    Ashish G Toshniwal, Geanette Lam ... Jared Rutter
    Pyruvate dictates redox balance and cell size, establishing metabolism as a dominant regulator of cellular fitness, unveiling new strategies to modulate cancer, regeneration, and diabetes.
    1. Neuroscience

    Strikingly different neurotransmitter release strategies in dopaminergic subclasses

    Ana Dorrego-Rivas, Darren J Byrne ... Matthew S Grubb
    Different locations of neurotransmitter release sites in axon-bearing and anaxonic olfactory bulb dopaminergic neurons allow self-inhibition only in anaxonic cells, showing how polarity differences create separate functional roles in sensory processing.
    1. Physics of Living Systems

    Postural adaptations may contribute to the unique locomotor energetics seen in hopping kangaroos

    Lauren Thornton, Taylor Dick ... Christofer J Clemente
    Postural adjustments that reduce ankle leverage in kangaroos enhance tendon stress and elastic energy storage at higher speeds, providing energetic efficiency during hopping but potentially constraining the maximum size achievable by large species.
    1. Immunology and Inflammation

    Immunoglobulin M regulates airway hyperresponsiveness independent of T helper 2 allergic inflammation

    Sabelo Hadebe, Anca Flavia Savulescu ... Frank Brombacher
    Immunoglobulin M has other functions beyond antibody class switching that have implications in asthma treatment.
    1. Cancer Biology

    Transcriptional subtypes on immune microenvironment and predicting postoperative recurrence and metastasis in human pheochromocytoma and paraganglioma

    Yang Liu, Xu Yan ... Lingyu Li
    Distinct transcriptional subtypes in PPGLs link molecular programs with immune context, providing biomarkers for recurrence risk and therapeutic targeting.
    1. Cancer Biology

    Endoscopic liquid biopsies of gastric fluid in a large human patient cohort reveal DNA content as a candidate tumor biomarker in gastric cancer

    Francine C Cadoná, Thais F Bartelli ... Emmanuel Dias-Neto
    Elevated gfDNA supports gastric cancer diagnosis and may guide management when combined with tissue biopsies, and in established cancer, higher gfDNA may predict better outcomes, potentially indicating immune-cell infiltration.
    1. Computational and Systems Biology
    2. Physics of Living Systems

    Exploiting fluctuations in gene expression to detect causal interactions between genes

    Euan Joly-Smith, Mir Mikdad Talpur ... Andreas Hilfinger
    Analyzing stochastic cell-to-cell variability can potentially reveal causal interactions in gene regulatory networks.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes

    Yury S Bykov, Solene Zuttion ... Maya Schuldiner
    Combined with high-throughput yeast genetics, a split green fluorescent protein encoded directly in the mitochondrial DNA helps to identify targeting signals and functions of dually localized proteins.
    1. Medicine
    2. Computational and Systems Biology

    Global molecular landscape of early MASLD progression in obesity

    Qing Zhao, William De Nardo ... Philipp Kaldis
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    1. Neuroscience

    Single molecule counting detects low-copy glycine receptors in hippocampal and striatal synapses

    Serena Camuso, Yana Vella ... Christian G Specht
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Epidemiology and Global Health

    Subnational tailoring of ITN distributions to maximise malaria control

    Andrew C Glover, Hannah Koenker ... Thomas S Churcher
    Not revised
    Reviewed Preprint v1
    • Useful
    • Convincing
    1. Immunology and Inflammation
    2. Stem Cells and Regenerative Medicine

    IL-27 limits HSPC differentiation during infection and protects from stem cell exhaustion

    Daniel L Aldridge, Zachary Lanzar ... Christopher A Hunter
    Functional analyses showcase that in the absence of IL-27, hematopoietic stem and progenitor cells are more likely to become monocytes during toxoplasmosis, making them less responsive after infection.
    1. Neuroscience

    Early changes in the properties of CA3 engram cells explored with a novel viral tool in mice

    Dario Cupolillo, Noelle Grosjean ... Christophe Mulle
    A novel viral tool (FLEN) is designed, and used to monitor changes in the intrinsic and synaptic properties of engram cells quickly (3–6 hours) following encoding of a memory.
    1. Neuroscience

    Dissociable memory modulation mechanisms facilitate fear amnesia at different timescales

    Yinmei Ni, Ye Wang ... Jian Li
    The retrieval-extinction paradigm elicits a short-term fear amnesia that differs in cue specificity, timescale, and dependence on thought-control ability from the long-term amnesia believed to be associated with memory reconsolidation.
    1. Genetics and Genomics

    Chromatin accessibility variation provides insights into missing regulation underlying immune-mediated diseases

    Raehoon Jeong, Martha L Bulyk
    Analyzing population-level chromatin accessibility data shows added benefit over utilizing only gene expression data in elucidating the genetic mechanism of disease-associated variants.
    1. Neuroscience

    Spectral decomposition of local field potentials uncovers frequency-tuned gain modulation of working memory in primate visual system

    Majid Roshanaei, Mohammad Reza Daliri ... Behrad Noudoost
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    In-situ glial cell-surface proteomics identifies pro-longevity factors in Drosophila

    Madeline P Marques, Bo Sun ... Hongjie Li
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Microbiology and Infectious Disease

    The structural context of mutations in proteins predicts their effect on antibiotic resistance

    Anna G Green, Mahbuba Tasmin ... Maha R Farhat
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Structural Biology and Molecular Biophysics

    Lenacapavir-induced Lattice Hyperstabilization is Central to HIV-1 Capsid Failure at the Nuclear Pore Complex and in the Cytoplasm

    Arpa Hudait, Ryan C Burdick ... Gregory A Voth
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Linear and categorical coding units in the mouse gustatory cortex drive population dynamics and behavior in taste decision-making

    Liam Lang, Camelia Yuejiao Zheng ... Alfredo Fontanini
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Neuroscience

    Verbal Episodic Processing in Newborns

    Emma Visibelli, Ana Fló ... Silvia Benavides-Varela
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Solid
    1. Neuroscience

    Reactivation-coupled brain stimulation enables complete learning generalization

    Yibo Xie, Minmin Wang ... Ke Jia
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Developmental Biology
    2. Neuroscience

    Complimentary vertebrate Wac models exhibit phenotypes relevant to DeSanto-Shinawi Syndrome

    Kang-Han Lee, April M Stafford ... Daniel Vogt
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Virtual Brain Inference (VBI), a flexible and integrative toolkit for efficient probabilistic inference on whole-brain models

    Abolfazl Ziaeemehr, Marmaduke Woodman ... Meysam Hashemi
    The Virtual Brain Inference (VBI) toolkit enables efficient, accurate, and scalable Bayesian inference over whole-brain network models, improving parameter estimation, uncertainty quantification, and predictive modeling across neuroimaging modalities toward precision medicine.
    1. Cancer Biology

    Spatially defined multicellular functional units in colorectal cancer revealed from single cell and spatial transcriptomics

    Inbal Avraham-Davidi, Simon Mages ... Aviv Regev
    An integrative analysis of scRNA-seq and spatial transcriptomics provides complementary views into the biology of pre-clinical colorectal cancer models in mice with possible applications to the human system.
    1. Neuroscience

    Separating the control of moving and holding in human post-stroke arm paresis

    Alkis M Hadjiosif, Kahori Kita ... John W Krakauer
    Patients with stroke often show impairment in both moving and holding the arm, deficits that are dissociable experimentally, suggesting that moving and holding are controlled by separate neural mechanisms.
    1. Ecology

    Range geography and temperature variability explain cross-continental convergence in range and phenology shifts in a model insect taxon

    Catherine Sirois-Delisle, Susan CC Gordon, Jeremy Kerr
    Across continents, half of odonate species shifted both ranges and phenologies in response to climate warming, with southern species and those experiencing less temperature variability shifting ranges more strongly.
    1. Neuroscience

    Sex-specific exploration accounts for differences in valence learning in male and female mice

    Heike Schuler, Eshaan S Iyer ... Rosemary C Bagot
    1. Cancer Biology
    2. Chromosomes and Gene Expression

    Degradation of LMO2 in T cell leukaemia results in collateral breakdown of transcription complex partners and causes LMO2-dependent apoptosis

    Naphannop Sereesongsaeng, Carole Bataille ... Terence Rabbitts
    Antibody surrogate degraders of LMO2 indicate that transcription complexes are susceptible to drug discovery and are therefore druggable.
    1. Cell Biology

    THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycle

    Taylar Hammond, Jong Bin Choi ... Julien Sage
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Microbiology and Infectious Disease

    Loss of the s2U tRNA modification induces antibiotic tolerance and is linked to changes in ribosomal protein expression

    Katherine L Cotten, Abigail McShane ... Kimberly M Davis
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Convincing
    1. Biochemistry and Chemical Biology

    A simplified and highly efficient cell-free protein synthesis system for prokaryotes

    Xianshengjie Lang, Changbin Zhang ... Wenfei Li
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Defining the chromatin-associated protein landscapes on Trypanosoma brucei repetitive elements using synthetic TALE proteins

    Roberta Carloni, Tadhg Devlin ... Robin C Allshire
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Biochemistry and Chemical Biology

    General trends in the calnexin-dependent expression and pharmacological rescue of clinical CFTR variants

    Austin Tedman, John A Olson III ... Jonathan P Schlebach
    Combining deep mutational scanning with CRISPR, interactome measurements, and electrophysiology reveals nuanced effects of the lectin chaperone calnexin on the pharmacological properties of 232 cystic fibrosis variants.
    1. Evolutionary Biology
    2. Neuroscience

    A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma

    Mireia Rovira, Giuliano Ferrero ... Valerie Wittamer
    The zebrafish brain combines a conserved heterogeneity of immune cells with a unique dendritic cell-like population.
    1. Cell Biology
    2. Developmental Biology

    The oocyte zinc transporter Slc39a10/Zip10 is a regulator of zinc sparks during fertilization in mice

    Atsuko Kageyama, Narumi Ogonuki ... Junya Ito
    The zinc transporter Slc39a10/Zip10 is important for zinc homeostasis during fertilization and embryonic development in mice.
    1. Physics of Living Systems

    Advantageous and disadvantageous inequality aversion can be taught through learning of others’ preferences

    Shen Zhang, Oriel FeldmanHall ... A Ross Otto
    Like disadvantageous inequity aversion, advantageous inequity aversion can be learned by observing another’s fairness preferences.
    1. Genetics and Genomics

    Functional analysis across model systems implicates ribosomal proteins in growth and proliferation defects associated with hypoplastic left heart syndrome

    Tanja Nielsen, Anaïs Kervadec ... Alexandre R Colas
    Cross-species functional genomics identify ribosomal protein genes as key regulators of cardiomyocyte proliferation and heart development, implicating them as candidate modifiers for HLHS and revealing new avenues for therapeutic discovery.
    1. Neuroscience

    State-dependent brain responsiveness, from local circuits to the whole brain

    Alain Destexhe, Jennifer S Goldman ... Maria V Sanchez-Vives
    Not revised
    Reviewed Preprint v1
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Noncanonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy

    Hao Chen, Haocheng Wang ... Jiou Wang
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Cell Biology

    Adaptation of endothelial cells to microenvironment topographical cues through lysyl oxidase like-2-mediated basement membrane scaffolding

    Marion F Marchand, Noémie Brassard-Jollive ... Laurent Muller
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Multiscale dynamical characterization of cortical brain states: from synchrony to asynchrony

    Maria V Sanchez-Vives, Arnau Manasanch ... Alain Destexhe
    Not revised
    Reviewed Preprint v1
    1. Microbiology and Infectious Disease

    The impact of different antimicrobial exposures on the gut microbiome in the ARMORD observational study

    Leon Peto, Nicola Fawcett ... Ann Sarah Walker
    Metagenomic sequencing of stool samples from 225 hospital patients and healthy volunteers reveals the impact of different antimicrobial exposures on the gut microbiome.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Dock-and-lock binding of SxIP ligands is required for stable and selective EB1 interactions

    Teresa Almeida, Eleanor Hargreaves ... Igor Barsukov
    Structural analysis reveals a two-step EB1-SxIP binding mechanism and identifies ligand mutations that greatly enhance affinity, offering potential therapeutic avenues for targeting microtubule plus end interactions.
    1. Neuroscience

    Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

    Jure Majnik, Manon Mantez ... Rosa Cossart
    A novel algorithm allowed for the continuous tracking of neurons throughout early postnatal development, revealing a transition in neural firing statistics coinciding with the onset of behaviour-dependent activity.
    1. Medicine

    Addressing cultural and knowledge barriers to enable preclinical sex inclusive research

    Brianna N Gaskill, Benjamin Phillips ... Natasha A Karp
    Targeted education corrects key misconceptions and identifies methods to influence intent to use sex-inclusive designs in in vivo studies.
    1. Cell Biology

    Evidence of centromeric histone 3 chaperone involved in DNA damage repair pathway in budding yeast

    Prakhar Agarwal, Anushka Alekar ... Santanu K Ghosh
    Genetic and biochemical assays reveal the role of Scm3 in genome stability by regulating the DNA damage checkpoint pathway in budding yeast.
    1. Neuroscience

    Robust and replicable effects of ageing on resting state brain electrophysiology measured with MEG

    Andrew J Quinn, Jemma Pitt ... Mark W Woolrich
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    1. Cell Biology

    Persistent contacts between Climp63 and microtubules cause mitotic defects and nuclear fragmentation

    Jelmi uit de Bos, Ulrike Kutay
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Developmental Biology
    2. Neuroscience

    The Fd4 transcription factor translates transient spatial cues in progenitors into long-term lineage identity

    Sen-Lin Lai, Chris Q Doe
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Computational and Systems Biology

    Thymic selection of the T cell receptor repertoire is biased toward autoimmunity in females

    Hélène Vantomme, Valentin Quiniou ... David Klatzmann
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Ecology

    Cardenolide toxin diversity impacts monarch butterfly growth and sequestration

    Anurag A Agrawal, Amy P Hastings, Paola Rubiano-Buitrago
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    EEG decodability of facial expressions and their stereoscopic depth cues in immersive virtual reality

    Felix Klotzsche, Ammara Nasim ... Michael Gaebler
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    Characterisation of cold-selective lamina I spinal projection neurons

    Aimi N Razlan, Wenhui Ma ... Junichi Hachisuka
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    1. Neuroscience

    How Occam’s razor guides human decision-making

    Eugenio Piasini, Shuze Liu ... Joshua I Gold
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Modulating task outcome value to mitigate real-world procrastination via noninvasive brain stimulation

    Zhiyi Chen, Zhilin Ren ... Tingyong Feng
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Continuous flashing suppression of V1 responses and the perceptual consequences revealed via two-photon calcium imaging and transformer modeling

    Cai-Xia Chen, Xin Wang ... Cong Yu
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Computational and Systems Biology
    2. Neuroscience

    A dynamic scale-mixture model of motion in natural scenes

    Jared M Salisbury, Stephanie E Palmer
    1. Stem Cells and Regenerative Medicine

    Glycogen engineering improves the starvation resistance of mesenchymal stem cells and their therapeutic efficacy in pulmonary fibrosis

    Yongyue Xu, Mamatali Rahman ... Qiong Wu
    Glycogen engineering strategies of mammalian cells were developed, which improved the survival of mesenchymal stem cells post-implantation and their therapeutic efficacy in pulmonary fibrosis model.
    1. Neuroscience

    Human neurocomputational mechanisms of guilt-driven and shame-driven altruistic behavior

    Ruida Zhu, Huanqing Wang ... Chao Liu
    Harm and responsibility differentially shape guilt and shame, which in turn drive compensatory behavior to varying extents.
    1. Neuroscience

    Frequency-selective contrast sensitivity modulation driven by fine-tuned exogenous attention at the foveal scale

    Yue Guzhang, T Florian Jaeger, Martina Poletti
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Neuroscience

    Learning-Associated Flexibility of Cortical Taste Coding Is Impaired in Shank3 Knockout Mice

    Chi-Hong Wu, Gina G Turrigiano
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Physics of Living Systems

    Theory of active self-organization of dense nematic structures in the actin cytoskeleton

    Waleed Mirza, Marco De Corato ... Marino Arroyo
    Theoretical and computational modeling reveals a mechanism of active patterning of uniform isotropic actin gels into dense nematic bundles, providing a physical basis for the adaptability of the actin cytoskeleton.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    A modular platform to display multiple hemagglutinin subtypes on a single immunogen

    Dana Thornlow Lamson, Faez Amokrane Nait Mohamed ... Aaron G Schmidt
    BOAS is a platform for building multivalent viral protein immunogens without a scaffold, such as a nanoparticle, to elicit broad influenza vaccine responses.
    1. Developmental Biology

    Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification

    Adiyant Lamba, Meng Zhu ... Magdalena Zernicka-Goetz
    Mouse embryo cells undergo apical-basal polarization asynchronously at the 8-cell stage, and the timing of polarization influences subsequent lineage allocation.
    1. Microbiology and Infectious Disease

    PPARγ mediated enhanced lipid biogenesis fuels Mycobacterium tuberculosis growth in a drug-tolerant hepatocyte environment

    Binayak Sarkar, Jyotsna Singh ... Rajesh S Gokhale
    In vitro, ex vivo, and in vivo studies reveal that hepatocytes are significantly infected by Mycobacterium tuberculosis which rewires the host cell metabolism to upregulate the lipid biosynthetic pathways.
    1. Neuroscience

    Rapid rebalancing of co-tuned ensemble activity in the auditory cortex

    HiJee Kang, Travis A Babola, Patrick O Kanold
    Holographic neuronal stimulation shows that auditory cortex neurons with shared functional properties rapidly adjust their responses during sensory processing, revealing a circuit-level mechanism that regulates overall network activity balance.
    1. Biochemistry and Chemical Biology

    Deep learning reveals endogenous sterols as allosteric modulators of the GPCR–Gα interface

    Sanjay Kumar Mohanty, Aayushi Mittal ... Gaurav Ahuja
    An AI-driven computational toolkit, Gcoupler, integrates ligand design, statistical modeling, and graph neural networks to predict endogenous metabolites that allosterically modulate the GPCR–Gα interface and regulate downstream signaling.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Serratia marcescens Outer Membrane Vesicles rapidly paralyze Drosophila melanogaster through triggering apoptosis in the nervous system

    Bechara Sina Rahme, Roberto E Bruna ... Dominique Ferrandon
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    • Incomplete
    1. Genetics and Genomics
    2. Cancer Biology

    Multimodal single-cell analyses reveal distinct fusion-regulated transcriptional programs in Ewing sarcoma

    Olivia G Waltner, April A Apfelbaum ... Scott N Furlan
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Epidemiology and Global Health

    Atovaquone/Proguanil Use and Zoster Vaccination Are Associated with Reduced Alzheimer’s Disease Risk in Two Cohorts: Implications for a Latent Toxoplasma gondii Mechanism

    Ariel Israel, Abraham Weizman ... Eugene Merzon
    Not revised
    Reviewed Preprint v1
    • Useful
    • Inadequate
    1. Developmental Biology

    Junctional and Actomyosin Dynamics Drive Endothelial Cell Rearrangements during Vascular Tube Formation

    Ludovico Maggi, Jianmin Yin ... Heinz-Georg Belting
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Neuroscience

    Sex differences in BNST signaling and BNST CRF in fear processing

    Olivia J Hon, Sofia Neira ... Thomas L Kash
    Critical differences in how the BNST responds to different types of fear across male and female mice.
    1. Neuroscience

    Neural correlates and reinstatement of recent and remote memory in children and young adults

    Iryna Schommartz, Philip F Lembcke ... Yee Lee Shing
    Children show less robust memory retention overnight and after 2 weeks, with memories transforming more rapidly from detailed to gist-like neural representations, revealing less stable consolidation mechanisms than in adults.
    1. Evolutionary Biology

    Trained immunity and immune priming in plants and invertebrates

    Joachim Kurtz, Raul Andino ... Jurriaan Ton
    An evolutionary perspective on immune priming across plants and invertebrates highlights the roles of microbiomes and epigenetic regulation in shaping innate immune memory with promising applications in agriculture and aquaculture.
    1. Cell Biology
    2. Developmental Biology

    Characterisation of cell-scale signalling by the core planar polarity pathway during Drosophila wing development

    Alexandre Carayon, Helen Strutt, David Strutt
    Quantitative Drosophila pupal wing experiments support planar polarity depending on cell-scale signalling that does not involve depletion of a limited pool of core proteins or polarised transport on microtubules.
    1. Genetics and Genomics

    Stranded short nascent strand sequencing reveals the topology of DNA replication origins in Trypanosoma brucei

    Slavica Stanojcic, Bridlin Barckmann ... Yvon Sterkers
    Not revised
    Reviewed Preprint v1
    • Inadequate
    1. Cell Biology

    Hepatic Transthyretin knockdown alleviates NAFLD by enhancing SERCA2 function and inhibiting endoplasmic reticulum stress

    Yingzi He, Tian Yang ... Fenping Zheng
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    • Incomplete
    1. Neuroscience

    Microsaccades track shifting but not necessarily maintaining covert visual-spatial attention

    Anna M van Harmelen, Freek van Ede
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    Cholinergic blockade reveals a role for human hippocampal theta in memory encoding but not retrieval

    Tamara Gedankien, Jennifer Kriegel ... Joshua Jacobs
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience
    2. Physics of Living Systems

    Sub-surface deformation of individual fingerprint ridges during tactile interactions

    Giulia Corniani, Zing S Lee ... Hannes P Saal
    Sub-surface imaging demonstrates that vertical shear beneath fingertip ridges enables high mechanical spatial resolution to support tactile sensing.
    1. Evolutionary Biology

    Coenzyme-protein interactions since early life

    Alma Carolina Sanchez Rocha, Mikhail Makarov ... Klára Hlouchová
    Amino acids that were abundant since the prebiotic era are enriched in protein binding sites of the most ancient coenzymes, supporting the plausibility of an early coenzyme–peptide world.
    1. Structural Biology and Molecular Biophysics

    Crossover in aromatic amino acid interaction strength between tyrosine and phenylalanine in biomolecular condensates

    David De Sancho, Xabier Lopez
    Hydration modulates aromatic interactions, explaining why Tyr is a stronger sticker than Phe in aqueous environments like protein condensates but not in the cores of folded proteins.
    1. Neuroscience

    Biophysical network modeling of temporal and stereotyped sequence propagation of neural activity in the premotor nucleus HVC

    Zeina Bou Diab, Marc Chammas, Arij Daou
    Songbird HVC sequences arise from a balance of ionic currents and structured inhibition, providing a mechanistic framework for understanding cortical sequence generation.
    1. Developmental Biology
    2. Neuroscience

    Ptbp1 is not required for retinal neurogenesis and cell fate specification

    Haley Appel, Rogger P Carmen-Orozco ... Seth Blackshaw
    Loss of function of Ptbp1 in retinal progenitors leads to changes in RNA splicing but does not affect neurogenesis and cell fate specification.
    1. Cell Biology
    2. Immunology and Inflammation

    Extracellular matrices regulate extravasation journey of leukocytes and inflammatory tissue fate

    Yu-Tung Li
    A holistic narrative describes how extracellular matrices regulate various aspects of the entire leukocyte journey from blood to inflamed tissue, and the subsequent functional impacts on leukocyte and tissue fates.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules

    Samantha J Cassell, Weiyan Li ... Jonathan R Whicher
    Cryo-EM and electrophysiology reveal the mechanism of SK2 channel modulation by the bee toxin apamin and small molecules, providing insights that enable the rational design of targeted SK2 channel therapeutics.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Timely vaccine strain selection and genomic surveillance improve evolutionary forecast accuracy of seasonal influenza A/H3N2

    John Huddleston, Trevor Bedford
    Reducing vaccine development time from 12 months to 6 reduces forecasting error by 25%, while reducing lags in genome sequence submission reduces uncertainty of current clade frequencies by 50%.
    1. Genetics and Genomics
    2. Computational and Systems Biology

    Evolutionary remodeling of non-canonical ORF translation in mammals

    Yue Chang, Tianyu Lei ... Hong Zhang
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Computational and Systems Biology
    2. Neuroscience

    The electrogenicity of the Na+/K+-ATPase poses challenges for computation in highly active spiking cells

    Liz Weerdmeester, Jan-Hendrik Schleimer, Susanne Schreiber
    The electrogenic Na+/K+-ATPase generates a slow activity-dependent feedback signal that influences the computation of excitable cells and requires additional compensatory mechanisms during periods of high sustained activity.
    1. Evolutionary Biology
    2. Genetics and Genomics

    The primate Major Histocompatibility Complex as a case study of gene family evolution

    Alyssa Lyn Fortier, Jonathan K Pritchard
    The Major Histocompatibility Complex region evolves via gene birth-and-death, resulting in short-lived genes, rapidly expanding gene subfamilies, and many gene fragments.
    1. Cell Biology

    Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity

    Hannah Neiswender, Jessica E Pride ... Graydon B Gonsalvez
    Disease-associated mutations in BICD2 result in loss- and gain-of-function interactions and dynein hyperactivity.
    1. Neuroscience

    Dorsal hippocampus mediates light–tone associations in male mice

    Julia S Pinho, Carla Ramon-Duaso ... Arnau Busquets-Garcia
    A light–tone sensory preconditioning task in male mice is developed revealing sex differences and demonstrating that dorsal, but not ventral, hippocampal CaMKII-positive neurons encode innocuous stimulus associations.
    1. Microbiology and Infectious Disease

    Peptidoglycan recycling is critical for cell division, cell wall integrity and β-lactam resistance in Caulobacter crescentus

    Pia Richter, Anna Merz ... Martin Thanbichler
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    1. Neuroscience

    Dynamic regulation of mRNA acetylation at synapses by spatial memory in mouse hippocampus

    Hai-Qian Zhou, Zhen Zhu ... Dong-Min Yin
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Developmental Biology

    Eed controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest

    Tim Casey-Clyde, S John Liu ... David R Raleigh
    Eed, a core subunit of Polycomb repressive complex 2, is required for craniofacial osteoblast differentiation and mesenchymal proliferation after induction of the neural crest.
    1. Computational and Systems Biology
    2. Developmental Biology

    MorphoNet 2.0: An innovative approach for qualitative assessment and segmentation curation of large-scale 3D time-lapse imaging datasets

    Benjamin Gallean, Tao Laurent ... Emmanuel Faure
    A collaborative open-source platform enabling interactive 3D visualization, quantitative evaluation, and expert curation of complex biological morphologies to enhance the quality and reproducibility of large-scale imaging studies.
    1. Genetics and Genomics
    2. Evolutionary Biology

    Pre-Cambrian origin of envelope-carrying retrotransposons in metazoans

    Shashank Chary, Rippei Hayashi
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Microbiology and Infectious Disease

    Bivalent mRNA booster encoding virus-like particles elicits potent polyclass RBD antibodies in pre-vaccinated mice

    Chengcheng Fan, Alexander A Cohen ... Magnus AG Hoffmann
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    1. Neuroscience

    Distinct brain mechanisms support trust violations, belief integration, and bias in human-AI teams

    Luisa Roeder, Pamela Hoyte ... Johan N van der Meer
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    Individual differences in tail risk sensitive exploration using Bayes-adaptive Markov decision processes

    Tingke Shen, Peter Dayan
    A normative computational model of individual differences in mouse exploration driven by reward and threat uncertainty as well as risk sensitivity when faced with a novel object in an open field.
    1. Genetics and Genomics
    2. Stem Cells and Regenerative Medicine

    Joint profiling of cell morphology and gene expression during in vitro neurodevelopment

    Adithi Sundaresh, Dimitri Meistermann ... Helena Kilpinen
    Molecular and morphological profiles of developing neurons in vitro uncover the cellular dynamics of human neurodevelopment and related disease.
    1. Neuroscience

    Complex opioid-driven modulation of glutamatergic and cholinergic neurotransmission in a GABAergic brain nucleus associated with emotion, reward, and addiction

    Ramesh Chittajallu, Anna Vlachos ... Chris J McBain
    A pharmacogenetic approach provides a foundational dataset describing the influence of opioids on synaptic processing in a brain microcircuit known to be critical for driving hedonic behaviors.
    1. Neuroscience

    Cerebellar Purkinje cell stripe patterns reveal a differential vulnerability and resistance to cell loss during normal aging in mice

    Sarah G Donofrio, Cheryl Brandenburg ... Roy V Sillitoe
    Purkinje cell subpopulations have varying vulnerability to age-related neurodegeneration, creating a striped pattern of cell loss that reflects the developmental, anatomical, and functional organization of the cerebellum.
    1. Neuroscience

    Cross-species standardised cortico-subcortical tractography

    Stephania Assimopoulos, Shaun Warrington ... Stamatios N Sotiropoulos
    By providing new ways to map cortico-subcortical connectivity patterns, the proposed cross-species tractography approaches directly allow novel comparative studies between the human and macaque brain and enable subsequent explorations of brain-behaviour/disease symptom associations across individuals.
    1. Neuroscience

    C. elegans food choice exhibits effort discounting-like behavior

    Jonathan RM Millet, Serge Faumont ... Shawn R Lockery
    The demonstration of effort discounting-like behavior in a lower invertebrate sets a new phylogenetic boundary on discounting behavior.
    1. Epidemiology and Global Health

    Using step selection functions to analyse human mobility using telemetry data in infectious disease epidemiology: a case study of leptospirosis

    Pablo Ruiz Cuenca, Fábio N Souza ... Emanuele Giorgi
    Step selection functions are a useful tool for analysing fine-scale human movements to understand environmental interactions in the context of infectious disease epidemiology.
    1. Cell Biology

    Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol

    Samuel Bru, Lydie Michaillat Mayer ... Andreas Mayer
    In vitro reconstitution identifies how lysosome-related vacuoles coordinate the synthesis and turnover of inorganic polyphosphates to create a powerful buffer system stabilising a vital metabolic parameter, the cytosolic concentration of phosphate.
    1. Neuroscience

    Nanophysiology approach reveals diversity in calcium microdomains across zebrafish retinal bipolar ribbon synapses

    Nirujan Rameshkumar, Abhishek P Shrestha ... Thirumalini Vaithianathan
    Calcium levels and structural diversity among synaptic ribbons in bipolar neurons likely enable differential encoding of visual information, revealing a potential mechanism for fine-tuning sensory signal processing.

Magazine

  1. Blurry photo of a flag, fish and a bird in the water

    Episode 101: December 2025

    1. Cancer Biology

    Breast Cancer: Slowing the growth of drug-resistant tumors

    Yingyi Zhang, Qianying Lu
    1. Neuroscience

    Experience-Dependent Plasticity: The little brain supervises learning in the big brain

    Martha Garcia, Mark Wagner
    1. Microbiology and Infectious Disease

    Tuberculosis: A surprising new hiding place for a dangerous pathogen

    Sheetal Gandotra, Yogendra Singh
    1. Neuroscience

    Neuronal Activity: Keeping track of moving targets

    Renata Batista-Brito, Geoffrey Terral