September 2025

Cover articles

    1. Plant Biology

    Inside a thylakoid membrane

    Wojciech Wietrzynski, Lorenz Lamm ... Benjamin D Engel
    1. Physics of Living Systems

    The benefits of disorder

    Wencheng Ji, Ori Hachmo ... Ariel Amir
    1. Microbiology and Infectious Disease

    Enzyme production in fungi

    Ayaka Itani, Haruto Motomura ... Norio Takeshita
    1. Evolutionary Biology

    Genetic parallels

    Oliver Voigt, Magdalena V Wilde ... Gert Wörheide

Highlights controls:

Research articles

    1. Neuroscience

    Multiple event segmentation mechanisms in the human brain

    Tan T Nguyen, Joset A Etzel ... Jeffrey M Zacks
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Ecology
    2. Physics of Living Systems

    A tale of two birds: cognitive simplicity drives collective route improvements in homing pigeons

    Shoubhik Chandan Banerjee, Fritz A Francisco, Albert B Kao
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Evolutionary Biology

    Viral genome sequence datasets display pervasive evidence of strand-specific substitution biases that are best described using non-reversible nucleotide substitution models

    Rita Sianga-Mete, Penelope Hartnady ... Darren P Martin
    Non-reversible nucleotide substitution models best describe strand-specific nucleotide substitution biases of viral genomes.
    1. Ecology

    Pesticide-induced resurgence in brown planthoppers is mediated by action on a suite of genes that promote juvenile hormone biosynthesis and female fecundity

    Yang Gao, Shao-Cong Su ... Shun-Fan Wu
    Sublethal emamectin benzoate alters juvenile hormone-regulating genes to boost brown planthopper fecundity and cause resurgence.
    1. Cell Biology

    Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI

    Alexander N Kapustin, Sofia Serena Tsakali ... Catherine M Shanahan
    Fibronectin triggers secretion of extracellular vesicles carrying collagen VI that regulate smooth muscle cell adhesion and migration during vascular repair and atherogenesis.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Non-cognate immunity proteins provide broader defenses against interbacterial effectors in microbial communities

    Abigail Knecht, Denise Sirias ... Karine A Gibbs
    A biochemical and bioinformatics approach shows how T6SS immunity proteins bind to and neutralize a range of non-cognate effectors, which is critical for promoting bacterial fitness in mixed-strain environments.
    1. Neuroscience

    Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants

    Balázs István Tóth, Bahar Bazeli ... Thomas Voets
    Rapamycin, competing with other rapalogs, activates TRPM8 via directly binding to a novel binding site, which results in providing new insights into the mechanisms of TRPM8 activation.
    1. Neuroscience

    Nonlinear feedback modulation contributes to the optimization of flexible decision-making

    Xuanyu Wu, Yang Zhou
    Precise feedback connections in posterior parietal cortex enable iterative computation between decision processes, optimizing flexible decision-making reliability.
    1. Neuroscience

    Observational activation of anterior cingulate cortical neurons coordinates hippocampal replay in social learning

    Xiang Mou, Daoyun Ji
    Neurons selective to running trajectories in the rat anterior cingulate cortex are reactivated during observation and interact with hippocampal replay to guide subsequent spatial navigation.
    1. Evolutionary Biology
    2. Physics of Living Systems

    Adaptations in wing morphology rather than wingbeat kinematics enable flight in small hoverfly species

    Camille Le Roy, Nina Tervelde ... Florian T Muijres
    Small hoverflies compensate for size-related flight challenges through evolutionary changes in wing shape rather than altered wingbeat kinematics.
    1. Neuroscience

    Sparse innervation and local heterogeneity in the vibrissal corticostriatal projection

    Kenza Amroune, Lorenzo Fontolan ... Ingrid Bureau
    Functional connectivity mapping reveals sparse, specific, and largely complementary cortical innervation of individual striatal medium spiny neurons within overlapping and loosely topographical projections from the barrel cortex.
    1. Cancer Biology
    2. Chromosomes and Gene Expression

    Telomeres control human telomerase (TERT) expression through non-telomeric TRF2

    Antara Sengupta, Soujanya Vinayagamurthy ... Shantanu Chowdhury
    Telomere length controls hTERT expression by modulating TRF2 distribution and PRC2-mediated repression, highlighting a self-regulatory mechanism in cancer.
    1. Physics of Living Systems

    Design principles of transcription factors with intrinsically disordered regions

    Wencheng Ji, Ori Hachmo ... Ariel Amir
    Intrinsically disordered regions in transcription factors enhance both target binding probability and search efficiency.
    1. Neuroscience

    Astrocytic modulation of population encoding in mouse visual cortex via GABA transporter 3 revealed by multiplexed CRISPR/Cas9 gene editing

    Jiho Park, Grayson O Sipe ... Mriganka Sur
    Astrocytic GABA transporter 3 is essential for preserving the integrity of neuronal population dynamics in accurately encoding and representing sensory information.
    1. Neuroscience

    Diverse calcium dynamics underlie place field formation in hippocampal CA1 pyramidal cells

    Mate Sumegi, Gaspar Olah ... Zoltan Nusser
    Two-photon Ca2+ imaging reveals that place fields of hippocampal place cells emerge with variable cellular mechanisms.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    A drug repurposing approach reveals targetable epigenetic pathways in Plasmodium vivax hypnozoites

    Steven P Maher, Malina A Bakowski ... Dennis E Kyle
    Drug repurposing screens reveal several epigenetic inhibitors as active against P. vivax hypnozoites demonstrating that epigenetic pathways play a central role in hypnozoite quiescence.
    1. Neuroscience

    Representation of male features in the female mouse accessory olfactory bulb, and their stability during the estrus cycle

    Oksana Cohen, Yoram Ben-Shaul
    Electrophysiological recordings in female mice show that response profiles of accessory olfactory bulb neurons remain stable during the course of the estrus cycle.
    1. Chromosomes and Gene Expression

    Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response

    Carmen Ruger-Herreros, Lucia Svoboda ... Bernd Bukau
    The nuclear J-domain protein Apj1 controls the shut-off phase of the yeast heat shock response by displacing DNA-bound heat shock transcription factor Hsf1.
    1. Microbiology and Infectious Disease
    2. Neuroscience

    Enterovirus D68 2A protease causes nuclear pore complex dysfunction and motor neuron toxicity

    Katrina M Zinn, Mathew W McLaren ... Matthew J Elrick
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    MORC2 Mediates Transcriptional Regulation Through Liquid-Liquid Phase Separation and DNA Binding

    Yanshen Zhang, Weiya Xu ... Yihui Bi
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    • Incomplete
    1. Microbiology and Infectious Disease
    2. Cancer Biology

    RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

    Ahmed Rishiq, Johanna Galski ... Ofer Mandelboim
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Physics of Living Systems

    Controlling the synchronization and symmetry breaking of coupled bacterial pili on active biofilm carpets

    Baha Altın, Yusuf Ilker Yaman ... Aşkın Kocabaş
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    • Incomplete
    1. Developmental Biology

    Inverted Assembly of the Lens Within Ocular Organoids Reveals Alternate Paths to Ocular Morphogenesis

    Elin Stahl, Miguel Angel Delgado-Toscano ... Lucie Zilova
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Cell Biology

    Identification of nuclear pore proteins at plasmodesmata: potential role in intercellular transport?

    T Moritz Schladt, Manuel Miras ... Wolf B Frommer
    Not revised
    Reviewed Preprint v1
    • Incomplete
    1. Neuroscience

    Intrinsic fluctuations in global connectivity reflect transitions between states of high and low prediction error

    Paul C Bogdan, Shenyang Huang ... Roberto Cabeza
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Developmental Biology

    Genetic Network Shaping Kenyon Cell Identity and Function in Drosophila Mushroom Bodies

    Pei-Chi Chung, Kai-Yuan Ku ... Hung-Hsiang Yu
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Convincing
    1. Cell Biology
    2. Computational and Systems Biology

    Lipid packing contributes to the confinement of caveolae to the plasma membrane

    Elin Larsson, Aleksei Kabedev ... Richard Lundmark
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    • Incomplete
    1. Neuroscience

    Spyglass: a framework for reproducible and shareable neuroscience research

    Kyu Hyun Lee, Eric L Denovellis ... Loren M Frank
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    • Incomplete
    1. Neuroscience

    Perceptual glimpses are locally accumulated and globally maintained at distinct processing levels

    Elisabeth Parés-Pujolràs, Anna C Geuzebroek ... Simon P Kelly
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Cell Biology
    2. Developmental Biology

    Quantitative computerized analysis demonstrates strongly compartmentalized tissue deformation patterns underlying mammalian heart tube formation

    Morena Raiola, Miquel Sendra ... Miguel Torres
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Biochemistry and Chemical Biology

    D-serine suppresses one-carbon metabolism by competing with mitochondrial L-serine transport

    Masataka Suzuki, Kenichiro Adachi ... Jumpei Sasabe
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Cell Biology

    A GCN1-independent activator of the kinase GCN2

    JiaYi Zhu, Giulia Emanuelli ... Stefan J Marciniak
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Spatial frequency adaptation modulates population receptive field sizes

    Ecem Altan, Catherine A Morgan ... D Samuel Schwarzkopf
    Modulations in population receptive field size induced by spatial frequency adaptation reveal a direct link between spatial tuning in the visual cortex and spatial frequency selectivity of neuronal populations.
    1. Structural Biology and Molecular Biophysics

    Ω-Loop mutations control dynamics of the active site by modulating the hydrogen-bonding network in PDC-3 β-lactamase

    Shuang Chen, Andrew R Mack ... Shozeb Haider
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Product-stabilized filamentation by human glutamine synthetase allosterically tunes metabolic activity

    Eric Greene, Richard Muniz ... James S Fraser
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    1. Evolutionary Biology

    Heterozygote advantage cannot explain MHC diversity, but MHC diversity can explain heterozygote advantage

    Joshua L Cherry
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Developmental Biology
    2. Neuroscience

    Segment-specific axon guidance by Wnt/Fz signaling diversifies motor commands in Drosophila larvae

    Suguru Takagi, Shiina Takano ... Akinao Nose
    Rewiring the command neurons in the tactile circuit is sufficient to change the behavioral strategy of a whole animal, implying a 'plug-and-play' flexibility in sensorimotor circuits.
    1. Cell Biology
    2. Neuroscience

    The effects of 17α-estradiol treatment on endocrine system revealed by single-nucleus transcriptomic sequencing of hypothalamus

    Lei Li, Guanghao Wu ... Yinchuan Li
    Long-term 17α-estradiol modulates aged male hypothalamic function via HPG axis and energy metabolism, mitigating aging-related neuronal changes.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    The molecular infrastructure of glutamatergic synapses in the mammalian forebrain

    Julia Peukes, Charlie Lovatt ... René AW Frank
    CryoCLEM-guided cryoET of Psd95-GFP knockin mouse tissue and ultra-fresh synaptonerosomes indicate that the postsynaptic density is a not a conserved feature of glutamatergic synapses in the mouse brain.
    1. Genetics and Genomics
    2. Neuroscience

    Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain

    Tom Bonnifet, Sandra Sinnassamy ... Julia Fuchs
    LINE-1-encoded ORF1p is widely expressed in neurons, increases with age, and engages neuron-specific protein partners, revealing a dynamic role for retrotransposons in brain physiology and aging.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    Nucleation-dependent propagation of Polycomb modifications emerges during the Drosophila maternal to zygotic transition

    Natalie Gonzaga-Saavedra, Eleanor A Degen ... Shelby A Blythe
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Cell Biology
    2. Immunology and Inflammation

    Extracellular matrices regulate extravasation journey of leukocytes and inflammatory tissue fate

    Yu-Tung Li
    Not revised
    Reviewed Preprint v1
    1. Genetics and Genomics
    2. Neuroscience

    Starvation transforms signal encoding in C. elegans thermoresponsive neurons and suppresses heat avoidance via bidirectional glutamatergic and peptidergic signaling

    Saurabh Thapliyal, Dominique A Glauser
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Synaptotagmin 1 and Synaptotagmin 7 promote MR1-mediated presentation of Mycobacterium tuberculosis antigens

    Se-Jin Kim, Jessie C Peterson ... David M Lewinsohn
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Computational and Systems Biology
    2. Evolutionary Biology

    Importance of higher-order epistasis in protein sequence-function relationships

    Palash Sethi, Juannan Zhou
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience
    2. Stem Cells and Regenerative Medicine

    Adrenomedullin restores the human cortical interneurons migration defects induced by hypoxia

    Alyssa Puno, Wojciech P Michno ... Anca M Pasca
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology

    Treacle and MDC1 coordinate rDNA break repair by homologous recombination

    Andrea Haenel, Johannes Leyrer, Manuel Stucki
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Neuroscience

    Online reinforcement learning of state representation in recurrent network supported by the power of random feedback and biological constraints

    Takayuki Tsurumi, Ayaka Kato ... Kenji Morita
    Recurrent neural network and its readout (cortex–striatum) can learn state representation and value using online random-weight feedback of temporal-difference reward-prediction-error (dopamine) through feedback alignment or biological non-negative-weight constraint-induced loose alignment.
    1. Cell Biology

    Ubiquitination-activated TAB–TAK1–IKK–NF-κB axis modulates gene expression for cell survival in the lysosomal damage response

    Akinori Endo, Chikage Takahashi ... Yukiko Yoshida
    Ubiquitin accumulated on damaged lysosomes activates TAB–TAK1–IKK–NF-κB axis and remodels proteome through gene expression for cell survival and cell-cell communication.
    1. Evolutionary Biology

    Forecasting protein evolution by integrating birth-death population models with structurally constrained substitution models

    David Ferreiro, Luis Daniel González-Vázquez ... Miguel Arenas
    Forecasting protein evolution in terms of folding stability using state-of-the-art population genetics and substitution models is feasible in rugged fitness landscapes, whereas forecasting exact amino acid sequences remains more challenging.
    1. Structural Biology and Molecular Biophysics

    Conformational changes, excess area, and elasticity of the Piezo protein-membrane nanodome from coarse-grained and atomistic simulations

    Sneha Dixit, Frank Noé, Thomas R Weikl
    Piezo proteins induce a curved protein-membrane nanodome with an excess area of about 40 nm2 in tensionless membranes and resist tension-induced flattening with a force constant of about 60 pN/nm.
    1. Structural Biology and Molecular Biophysics

    Dimerization and dynamics of human angiotensin-I converting enzyme revealed by cryo-EM and MD simulations

    Jordan M Mancl, Xiaoyang Wu ... Wei-Jen Tang
    Structural and computational approaches uncover the molecular basis for dimerization, open-closed conformational transitions, and the dynamic behavior of the human angiotensin-I converting enzyme dimer.
    1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    Macroscopic Analyses of RNA-Seq Data to Reveal Chromatin Modifications in Aging and Disease

    Achal Mahajan, Francesca Ratti ... Vishrawas Gopalakrishnan
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Altered cognitive processes shape tactile perception in autism

    Ourania Semelidou, Mathilde Tortochot-Megne Fotso ... Andreas Frick
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Canonical and phosphoribosyl ubiquitination coordinate to stabilize a proteinaceous structure surrounding the Legionella-containing vacuole

    Adriana Steinbach, Chetan Mokkapati, Shaeri Mukherjee
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    1. Neuroscience

    A Priming Circuit Controls the Olfactory Response and Memory in Drosophila

    He Yang, Yang Jiang, Samuel Kunes
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Computational and Systems Biology

    Regulation of Transcriptional Bursting and Spatial Patterning in Early Drosophila Embryo Development

    César Nieto, Zahra Vahdat ... Abhyudai Singh
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    A Python Toolbox for Representational Similarity Analysis

    Jasper JF van den Bosch, Tal Golan ... Heiko H Schütt
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Neuroscience

    Restoration of locomotor function following stimulation of the A13 region in Parkinson’s mouse models

    Linda H Kim, Adam Lognon ... Patrick J Whelan
    Stimulation of the A13 brain region restores motor function in Parkinson's disease mouse models by alleviating bradykinesia and akinesia.
    1. Cell Biology

    SARS-CoV-2 NSP13 interacts with TEAD to suppress Hippo-YAP signaling

    Fansen Meng, Jong Hwan Kim ... James F Martin
    A novel YAP/TEAD regulatory mechanism by NSP13 provides molecular insights into Hippo-YAP regulation after SARS-CoV-2 infection.
    1. Computational and Systems Biology
    2. Neuroscience

    Neural dynamics of reversal learning in the prefrontal cortex and recurrent neural networks

    Christopher M Kim, Carson C Chow, Bruno B Averbeck
    Neural activity during reversal learning encodes decision-related evidence integrated across trials and shows substantial dynamics during each trial, suggesting an extension of the line attractor model that incorporates non-stationary dynamics.
    1. Ecology
    2. Plant Biology

    Strip cropping shows promising increases in ground beetle community diversity compared to monocultures

    Luuk Croijmans, Fogelina Cuperus ... Erik H Poelman
    Strip cropping is a farm management practice that increases ground beetle diversity without major effects on crop yield and while being practically implementable.
    1. Neuroscience

    Oxytocin restores context-specific hyperaltruistic preference

    Hong Zhang, Yinmei Ni, Jian Li
    Oxytocin facilitates hyperaltruism by promoting harm-framing in the moral decision-making task.
    1. Neuroscience

    Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation

    Jie Wang, Jun Nishiyama ... Ryohei Yasuda
    Rab10 and Rab4 bidirectionally regulate AMPA receptor trafficking and spine structural plasticity during long-term potentiation by exhibiting opposing and temporally distinct activity patterns in stimulated dendritic spines.
    1. Structural Biology and Molecular Biophysics

    Multiphase separation in postsynaptic density regulated by membrane geometry via interaction valency and volume

    Risa Yamada, Giovanni B Brandani, Shoji Takada
    Membrane geometry switches protein condensate organization of postsynaptic density proteins by reducing crowding effects while maintaining specific binding, allowing targeted interactions to dominate over size-based exclusion.
    1. Neuroscience

    Hierarchical encoding of natural sound mixtures in ferret auditory cortex

    Agnès Landemard, Célian Bimbard, Yves Boubenec
    To parse foreground and background sounds, the auditory cortex of humans and ferrets share a similar hierarchical organization, but the underlying computational mechanisms are fundamentally different.
    1. Microbiology and Infectious Disease

    The increase in cell volume and nuclear number of the koji-fungus Aspergillus oryzae contributes to its high enzyme productivity

    Ayaka Itani, Haruto Motomura ... Norio Takeshita
    More nuclei and cell volume, more enzymes in koji-fungus Aspergillus oryzae.
    1. Cell Biology
    2. Developmental Biology

    Sulfation affects apical extracellular matrix organization during development of the Drosophila embryonic salivary gland tube

    J Luke Woodward, Jeffrey Matthew ... SeYeon Chung
    Sulfation is required for organizing the apical extracellular matrix during tubular organ formation.
    1. Medicine

    Peer reviewers altered their recommendation based on whether they were cited or wanted to be cited. A matched study of open peer review at four journals

    Adrian Barnett
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Genetics and Genomics

    Identifying the genetic basis and molecular mechanisms underlying phenotypic correlation between complex human traits using a gene-based approach

    Jialiang Gu, Chris Fuller ... Hao Li
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    The Dual Molecular Identity of Vestibular Kinocilia: Bridging Structural and Functional Traits of Primary and Motile Cilia

    Zhenhang Xu, Amirrasoul Tavakoli ... David Z He
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Cancer Biology

    Transcriptional Subtypes on Immune Microenvironment and Predicting Postoperative Recurrence and Metastasis in Pheochromocytoma and Paraganglioma

    Yang Liu, Xu Yan ... Lingyu Li
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Chromosomes and Gene Expression

    ATAD2 mediates chromatin-bound histone chaperone turnover

    Ariadni Liakopoulou, Fayçal Boussouar ... Saadi Khochbin
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Computational and Systems Biology

    Identification of type 2 diabetes- and obesity-associated human β-cells using deep transfer learning

    Gitanjali Roy, Rameesha Syed ... Michael A Kalwat
    Deep transfer learning methodologies reveal hidden heterogeneity in β-cells driven by type 2 diabetes and obesity.
    1. Microbiology and Infectious Disease

    A SHERLOCK toolbox for eco-epidemiological surveillance of African trypanosomes in domestic pigs from Western Africa

    Roger Eloiflin, Elena Pérez-Antón ... Brice Rotureau
    A novel array of CRISPR-based SHERLOCK tests for RNA detection was developed for eco-epidemiological surveillance of human and animal African trypanosomes from Western Africa with domestic pigs as sentinels.
    1. Neuroscience

    Intrinsic and circuit mechanisms of predictive coding in a grid cell network model

    Inayath Shaikh, Collins Assisi
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Neuroscience

    Executive Resources Shape the Impact of Language Predictability Across the Adult Lifespan

    Merle Schuckart, Sandra Martin ... Jonas Obleser
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Developmental Biology
    2. Neuroscience

    Ptbp1 is not required for retinal neurogenesis and cell fate specification

    Haley Appel, Rogger P Carmen-Orozco ... Seth Blackshaw
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    An updated catalogue of split-GAL4 driver lines for descending neurons in Drosophila melanogaster

    Jessica L Zung, Shigehiro Namiki ... Gwyneth M Card
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Cell Biology
    2. Neuroscience

    Maintenance of neuronal TDP-43 expression requires axonal lysosome transport

    Veronica H Ryan, Sydney Lawton ... Michael Emmerson Ward
    Protein, but not mRNA, levels of neurodegenerative disease-associated protein TDP-43 are decreased upon knockdown of BORC, a complex that is required for the anterograde transport of lysosomes.
    1. Neuroscience

    Human single-neuron activity is modulated by intracranial theta burst stimulation of the basolateral amygdala

    Justin M Campbell, Rhiannon L Cowan ... Jon Timothy Willie
    Firing rate analyses revealed neurons throughout the hippocampus, amygdala, orbitofrontal cortex, and anterior cingulate cortex in humans that exhibited heterogeneous responses to intracranial theta burst stimulation of the human brain.
    1. Microbiology and Infectious Disease

    Membrane binding properties of the cytoskeletal protein bactofilin

    Ying Liu, Rajani Karmakar ... Martin Thanbichler
    A combination of cell biological, biochemical, and computational approaches characterizes the conserved membrane-targeting sequence of bactofilins and reveals a mutual influence of membrane binding and bactofilin polymerization.
    1. Biochemistry and Chemical Biology

    Blue-shifted ancyromonad channelrhodopsins for multiplex optogenetics

    Elena G Govorunova, Oleg A Sineshchekov ... John L Spudich
    Ancyromonad channelrhodopsins advance understanding of ionic selectivity and wavelength regulation in light-gated ion channels, and expand the toolkit for multiplexing with red-shifted fluorescent sensors.
    1. Microbiology and Infectious Disease

    The vaccine candidate Liver Stage Antigen 3 is exported during Plasmodium falciparum infection and required for liver-stage development

    Robyn McConville, Ryan WJ Steel ... Justin A Boddey
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    Recovery of the full in vivo firing range in post-lesion surviving DA SN neurons associated with Kv4.3-mediated pacemaker plasticity

    Lora Kovacheva, Josef Shin ... Jochen Roeper
    1. Cell Biology

    Distinct functions of cardiac β-adrenergic receptors in the T-tubule vs. outer surface membrane

    George WP Madders, Marion Barthé ... Rodolphe Fischmeister
    A size exclusion strategy using PEGylation of isoprenaline allowed to demonstrate that cardiac β-adrenergic receptors located in the outer surface membrane control mainly cAMP/PKA in the cytosol while those located in the T-tubule membrane control cAMP/PKA in the nucleus.
    1. Cell Biology
    2. Neuroscience

    BetaII-Spectrin Gaps and Patches Emerge from the Patterned Assembly of the Actin/Spectrin Membrane Skeleton in Human Motor Neuron Axons

    Nahir Guadalupe Gazal, Maria Jose Castellanos-Montiel ... Nicolás Unsain
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Neuroscience

    A novel mouse model for LAMA2-related muscular dystrophy with analysis of molecular pathogenesis and clinical phenotype

    Dandan Tan, Yidan Liu ... Hui Xiong
    Analysis of phenotype and molecular pathogenesis in a new LAMA2-related muscular dystrophy mouse model.
    1. Cell Biology
    2. Genetics and Genomics

    Impact of maximal overexpression of a non-toxic protein on yeast cell physiology

    Yuri Fujita, Shotaro Namba ... Hisao Moriya
    A neutrality index identifies mox-YG as a minimally cytotoxic protein, enabling ultra-high expression and uncovering key physiological consequences of protein burden.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Fast evolutionary turnover and overlapping variances of sex-biased gene expression patterns defy a simple binary sex classification of somatic tissues

    Chen Xie, Sven Künzel, Diethard Tautz
    Sex-biased gene expression in the body organs evolves rapidly, supporting a mosaic model of maleness and femaleness across tissues and thus challenging binary views of sex characteristics in mammals.
    1. Biochemistry and Chemical Biology

    Coupled equilibria of dimerization and lipid binding modulate SARS Cov 2 Orf9b interactions and interferon response

    CJ San Felipe, Jyoti Batra ... James S Fraser
    Lipid binding stabilizes the SARS-CoV-2 Orf9b homodimer, slowing its conversion to the Tom70-binding monomeric form and modulating interferon suppression.
    1. Cancer Biology
    2. Medicine

    Monitoring circulating cell-free HPV DNA in metastatic or recurrent cervical cancer: clinical significance and treatment implications

    Zhuomin Yin, Tao Feng ... Hanmei Lou
    HPV cfDNA is a highly effective tumor marker for monitoring disease progression and treatment response in HPV-positive cervical cancer, offering valuable insights for guiding clinical decisions in precision medicine.
    1. Neuroscience

    In vivo mapping of striatal neurodegeneration in Huntington’s disease with Soma and Neurite Density Imaging

    Vasileios Ioakeimidis, Marco Palombo ... Claudia Metzler-Baddeley
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Cell Biology
    2. Immunology and Inflammation

    Protein phase change batteries drive innate immune signaling and cell fate

    Alejandro Rodriguez Gama, Tayla Miller ... Randal Halfmann
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    The ciliary kinesin KIF7 controls the development of the cerebral cortex by acting differentially on SHH signaling in dorsal and ventral forebrain

    María Pedraza, Valentina Grampa ... Justine Masson
    KIF7 loss of function affects several developmental stages of the cerebral cortex and likely causes neurodevelopmental delay, intellectual disability, and epilepsia observed in ciliopathic patients with KIF7 mutations.
    1. Neuroscience

    Structure transfer and consolidation in visual implicit learning

    Dominik Garber, József Fiser
    Sleep is essential for consolidating implicitly acquired perceptual knowledge that enables the knowledge transfer effect via newly learned structured information observed in prior studies of explicit learning.
    1. Microbiology and Infectious Disease

    Prophage regulation of Shewanella fidelis 3313 motility and biofilm formation with implications for gut colonization dynamics in Ciona robusta

    Ojas Natarajan, Susanne L Gibboney ... Larry J Dishaw
    Deleting SfPat prophage reduces Shewanella fidelis motility, increases biofilms, changes pdeB expression and spatial retention in Ciona gut, while Ciona VCBP-C binding to the WT strain results in reduced expression of viral capsid proteins without inducing SOS, indicating tripartite interactions.
    1. Neuroscience

    Novel and optimized mouse behavior enabled by fully autonomous HABITS: Home-cage assisted behavioral innovation and testing system

    Bowen Yu, Penghai Li ... Yaoyao Hao
    Fully autonomous home-cage training system HABITS enables mice to learn complex cognitive behaviors without human intervention, expanding behavioral paradigms and optimizing training efficiency through machine teaching algorithms.
    1. Evolutionary Biology
    2. Physics of Living Systems

    Magnetotactic bacteria optimally navigate natural pore networks

    Alexander P Petroff, Julia Hernandez ... Nina Radchenko-Hannafin
    Diverse magnetotactic bacteria are adapted to the local geomagnetic field and grain size to maximize their swimming speed through the pore space.
    1. Cancer Biology
    2. Computational and Systems Biology

    Altered thymic niche synergistically drives the massive proliferation of malignant thymocytes

    Erika Tsingos, Advaita M Dick, Baubak Bajoghli
    Combining computer modeling and experiments in animals shows that the spatial organization of thymic epithelial cells affects the availability of signaling molecules that regulate thymocyte proliferation and development.
    1. Cell Biology

    Orderly mitosis shapes interphase genome architecture

    Krishnendu Guin, Adib Keikhosravi ... Tom Misteli
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Physics of Living Systems

    Transport kinetics across interfaces between coexisting liquid phases

    Lars Hubatsch, Stefano Bo ... Frank Jülicher
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    Spatially Periodic Computation in the Entorhinal-Hippocampal Circuit During Navigation

    Bo Zhang, Xin Guan ... Jia Liu
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Immunology and Inflammation
    2. Computational and Systems Biology

    Restoring data balance via generative models of T cell receptors for antigen-binding prediction

    Emanuele Loffredo, Mauro Pastore ... Rémi Monasson
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Evolutionary Biology

    The success of artificial selection for collective composition hinges on initial and target values

    Juhee Lee, Wenying Shou, Hye Jin Park
    Mathematical modeling uncovers how initial and target compositions limit microbial community selection, as inter-community selection clashes with intra-community selection like a rafter battling a waterfall.
    1. Structural Biology and Molecular Biophysics

    Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution

    Adrián Merino-Salomón, Jonathan Scheneider ... German Rivas
    Promoting FtsZ toroidal polymer formation by ZapD offers new insights into stabilizing the bacterial division ring.
    1. Cancer Biology

    SETD2 suppresses tumorigenesis in a KRASG12C-driven lung cancer model, and its catalytic activity is regulated by histone acetylation

    Ricardo J Mack, Natasha M Flores ... Or Gozani
    Genetic and biochemical studies show that SETD2 is tumor suppressive in a lung cancer mouse model and SETD2 methylation activity in vitro is regulated by histone acetylation.
    1. Cell Biology
    2. Evolutionary Biology

    Horizontally transferred cell-free chromatin particles function as autonomous ‘satellite genomes’ and vehicles for transposable elements within host cells

    Soumita Banerjee, Soniya Sanjay Shende ... Indraneel Mittra
    Uptake of cell-free chromatin particles released from dying cells and carrying non-coding DNA could redefine mammalian genomics.
    1. Computational and Systems Biology

    Anatomical basis of sex differences in the electrocardiogram identified by three-dimensional torso-heart imaging reconstruction pipeline

    Hannah J Smith, Blanca Rodriguez ... Abhirup Banerjee
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    • Incomplete
    1. Structural Biology and Molecular Biophysics

    Allosteric effects of the coupling cation in melibiose transporter MelB

    Parameswaran Hariharan, Yuqi Shi ... Lan Guan
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    1. Neuroscience
    2. Evolutionary Biology

    Distinct evolutionary trajectories of two integration centres, the central complex and mushroom bodies, across Heliconiini butterflies

    Max S Farnworth, Yi Peng Toh ... Stephen H Montgomery
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Neuroscience

    Multimodal MRI Marker of Cognition Explains the Association Between Cognition and Mental Health in UK Biobank

    Irina Buianova, Mateus Silvestrin ... Narun Pat
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Immunology and Inflammation

    Depletion of extracellular asparagine impairs self-reactive T cells and ameliorates autoimmunity in a murine model of multiple sclerosis

    Peter Georgiev, Sheila Johnson ... Arlene H Sharpe
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    The spatial frequency representation predicts category coding in the inferior temporal cortex

    Ramin Toosi, Behnam Karami ... Mohammad-Reza A Dehaqani
    Spatial frequency is explicitly and dynamically represented in the primate inferior temporal cortex, revealing distinct neural populations that differentially support object recognition, specially emphasizing high-frequency contributions to face processing.
    1. Neuroscience

    Policy shaping based on the learned preferences of others accounts for risky decision-making under social observation

    HeeYoung Seon, Dongil Chung
    Simulating others' preferences underlies riskier choices when being observed, driven by beliefs about observers' risk attitudes and supported by TPJ–dmPFC connectivity.
    1. Neuroscience

    Sequence action representations contextualize during early skill learning

    Debadatta Dash, Fumiaki Iwane ... Leonardo G Cohen
    When learning a new skill, neural representations of individual actions rapidly incorporate contextual information pertaining to the encompassing skill sequence-primarily during rest breaks interleaved with practice.
    1. Microbiology and Infectious Disease

    Tuberculosis susceptibility in genetically diverse mice reveals functional diversity of neutrophils

    Marietta M Ravesloot-Chavez, Erik Van Dis ... Sarah A Stanley
    Wild-derived mice exhibit diversity in outcomes after infection with Mycobacterium tuberculosis.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Ancient trans-species polymorphism at the Major Histocompatibility Complex in primates

    Alyssa Lyn Fortier, Jonathan K Pritchard
    Trans-species polymorphism at primate Major Histocompatibility Complex genes is old, especially in the 'classical' genes, while rapidly-evolving regions of each gene correspond with proteins' functional domains.
    1. Evolutionary Biology

    The general version of Hamilton’s rule

    Matthijs van Veelen
    The flexibility of the general version of Hamilton's rule allows the rule to match the complexity of the model of social behaviour, or the complexity implied by the data.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Spatial and longitudinal tracking of enhancer-AAV vectors that target transgene expression to injured mouse myocardium

    David W Wolfson, Joshua A Hull ... Kenneth D Poss
    Bioluminescence imaging in live mice was used to assess the abilities of systemically introduced adeno-associated viral capsids containing enhancer regulatory sequences to target transgene expression to myocardial injuries.
    1. Cell Biology
    2. Developmental Biology

    Gene regulatory mechanisms guiding bifurcation of inhibitory and excitatory neuron lineages in the mouse anterior brainstem

    Sami Kilpinen, Lassi Virtanen ... Juha Partanen
    Changes of the chromatin structure suggests how the fate selector transcription factors are activated and how they guide differentiation of GABAergic and glutamatergic neurons in the anterior brainstem.
    1. Microbiology and Infectious Disease
    2. Epidemiology and Global Health

    Associations Between Meteorological Factors and Influenza A/B Incidence in Subtropical China: A Six-Year Surveillance Study with Deep Learning Modelling for Influenza Early Warning

    Long Xie, Meng-Jie Zhang ... Xi-Wen Jiang
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Microbiology and Infectious Disease

    Starvation of the bacteria Vibrio atlanticus promotes lightning group-attacks on the dinoflagellate Alexandrium pacificum

    Jean Luc Rolland, Estelle Masseret ... Raphaël Lami
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    • Incomplete
    1. Neuroscience

    Selective life-long suppression of an odor processing channel in response to critical period experience

    Hans C Leier, Julius Jonaitis ... Andrew M Dacks
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    • Solid
    1. Developmental Biology

    A cell atlas of the developing human outflow tract of the heart and its adult derivatives

    Rotem Leshem, Syed Murtuza Baker ... Nicoletta Bobola
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Modeling metabolic disease susceptibility and resilience in genetically diverse mice

    Candice N Baker, Jeffrey M Harder ... Gary A Churchill
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    • Compelling
    1. Neuroscience

    Challenges in Replay Detection by TDLM in Post-Encoding Resting State

    Simon Kern, Juliane Nagel ... Gordon Feld
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Cell Biology

    Large-scale identification of plasma membrane repair proteins revealed spatiotemporal cellular responses to plasma membrane damage

    Yuta Yamazaki, Keiko Kono
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology

    Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

    Scott D Zawieja, Grace A Pea ... Michael J Davis
    Lymphatic muscle cells, but not CD34+ adventitial cells, exhibited pacemaker behaviors including pressure-dependent depolarization, pressure-dependent calcium mobilization during diastole, and propagated contraction waves induced by focal, optogenetic depolarization via channel rhodopsin2.
    1. Neuroscience

    Mining the neuroimaging literature

    Jérome Dockès, Kendra M Oudyk ... Jean-Baptiste Poline
    New tools for literature mining, such as automated analysis of the research literature, are accessible, scalable, and reliable.
    1. Cancer Biology
    2. Developmental Biology

    Dissecting infant leukemia developmental origins with a hemogenic gastruloid model

    Denise Ragusa, Chun Wai Suen ... Cristina Pina
    The hemogenic gastruloid model of embryonic blood development captures multi-wave haematopoiesis and enables interrogation of infant leukaemia cell-of-origin.
    1. Plant Biology

    Molecular architecture of thylakoid membranes within intact spinach chloroplasts

    Wojciech Wietrzynski, Lorenz Lamm ... Benjamin D Engel
    Cryo-electron tomography reveals plant photosynthetic membranes with single-complex precision, supporting a simple two-domain model of membrane organization.
    1. Neuroscience

    Dynamic estimation of the attentional field from visual cortical activity

    Ilona M Bloem, Leah Bakst ... Sam Ling
    Visuocortical activity in humans reveals that the spatial focus of covert attention flexibly shifts and expands in accordance with task demands.
    1. Evolutionary Biology

    Structural evolution of nitrogenase over 3 billion years

    Bruno Cuevas Zuviría, Franka Detemple ... Betül Kaçar
    Structural reconstruction of thousands of ancient nitrogenases through Earth’s history uncovers architectural constraints that shaped their evolution under global environmental transitions.
    1. Immunology and Inflammation

    Xcr1+ type 1 conventional dendritic cells are essential mediators for atherosclerosis progression

    Tianhan Li, Liaoxun Lu ... Lichen Zhang
    Using Xcr1Cre-Gfp Rosa26LSL-DTA Apoe–/– model and Xcl1–/– Apoe–/– mouse model reveal an essential non-redundant role for the XCL1-XCR1 axis in cDC1-mediated atherogenesis.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Genome reorganization and its functional impact during breast cancer progression

    Kathleen S Metz Reed, Andrew Fritz ... Tom Misteli
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Convincing
    1. Immunology and Inflammation

    Type I and type III interferon receptor knockout chickens: Novel models for unraveling interferon dynamics in influenza infection

    Mohanned Naif Alhussien, Hanna-Kaisa Vikkula ... Benjamin Schusser
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    • Incomplete
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Dissecting organoid-bacteria interaction highlights decreased contractile force as a key factor for heart infection

    Anheng Wang, Jiaxian Wang ... Chunming Wang
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Structural Biology and Molecular Biophysics

    In silico design and validation of high-affinity RNA aptamers for SARS-CoV-2 comparable to neutralizing antibodies

    Yanqing Yang, Lulu Qiao ... Ruhong Zhou
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Cell Biology
    2. Immunology and Inflammation

    Male-Biased Cyp17a2 Governs Antiviral Sexual Dimorphism in Fish via STING Stabilization and Viral Protein Degradation

    Long-Feng Lu, Bao-jie Cui ... Shun Li
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Developmental Biology
    2. Genetics and Genomics

    A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

    Yifan Shen, Ann T Yeung ... Chun Han
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Genetics and Genomics
    2. Immunology and Inflammation

    Inhibition of the UFD-1-NPL-4 complex triggers an aberrant immune response in Caenorhabditis elegans

    Rajneesh Rao, Alejandro Aballay, Jogender Singh
    Inhibition of the UFD-1-NPL-4 complex activates immune responses in Caenorhabditis elegans that reduce gut pathogen load but simultaneously compromise host survival.
    1. Computational and Systems Biology
    2. Immunology and Inflammation

    The flexible stalk domain of sTREM2 modulates its interactions with brain-based phospholipids

    David Saeb, Emma E Lietzke ... Kayla G Sprenger
    The flexible stalk region of sTREM2 binds the Ig-like domain, altering stability and ligand accessibility—a biochemically testable mechanism with broad implications for (s)TREM2 biology and rational Alzheimer's disease therapeutic design.
    1. Structural Biology and Molecular Biophysics

    p53 isoforms have a high aggregation propensity, interact with chaperones and lack binding to p53 interaction partners

    Anamari Brdar, Christian Osterburg ... Volker Dötsch
    p53 isoforms induce cellular stress based on their only partially folded states.
    1. Computational and Systems Biology
    2. Neuroscience

    Accept–reject decision-making revealed via a quantitative and ethological study of C. elegans foraging

    Jessica A Haley, Tianyi Chen ... Sreekanth H Chalasani
    C. elegans foraging decisions are guided by learned environmental features, integrating recent patch quality with internal satiety to balance exploration and exploitation.
    1. Cancer Biology
    2. Immunology and Inflammation

    An evaluation of the tumor microenvironment through CALR, IL1R1, IFNB1, and IFNG to assess prognosis and immunotherapy response in bladder cancer patients

    Lilong Liu, Zhenghao Liu ... Zheng Liu
    A novel risk-scoring model enables personalized treatment decisions for bladder cancer patients.
    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    Human cytomegalovirus infection coopts chromatin organization to diminish TEAD1 transcription factor activity

    Khund Sayeed, Sreeja Parameswaran ... Matthew T Weirauch
    Infection of human cells by human cytomegalovirus induces massive changes to human chromatin and reduction of TEAD1 activity.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Regulatory networks of KRAB zinc finger genes and transposable elements changed during human brain evolution and disease

    Yao-Chung Chen, Arnaud Maupas, Katja Nowick
    Primate cross-species analysis of interactions between transposable elements and KRAB-ZNF genes with the new TEKRABber tool highlights human-specific interactions with potential relevance to Alzheimer’s disease.
    1. Microbiology and Infectious Disease

    The second messenger signaling molecule cyclic di-AMP drives developmental cycle progression in Chlamydia trachomatis

    Junghoon Lee, Scot P Ouellette
    For the first time, a function for the second messenger molecule, cyclic di-AMP, in Chlamydia has been described, revealing its role in regulating chlamydial developmental cycle progression.
    1. Neuroscience

    Scheduled feeding improves behavioral outcomes and reduces inflammation in a mouse model of fragile X syndrome

    Huei-Bin Wang, Natalie E Smale ... Christopher S Colwell
    These findings provide convincing evidence of the beneficial effects that restored biological rhythms may have on disease pathophysiology and symptoms.
    1. Immunology and Inflammation

    Tolerance to Lung Infection in TWIK2 K+ Efflux Mediated Macrophage Trained Immunity

    Josh Thompson, Yufan Li ... Jingsong Xu
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    How attention simplifies mental representations for planning

    Jason da Silva Castanheira, Nicholas Shea, Stephen M Fleming
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Realistic coupling enables flexible macroscopic traveling waves in the mouse cortex

    Guanhua Sun, James Hazelden ... Daniel B Forger
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Microbiology and Infectious Disease
    2. Evolutionary Biology

    Host and antibiotic jointly select for greater virulence in Staphylococcus aureus

    Michelle Su, Kim L Hoang ... Timothy D Read
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments

    Annemiek C Veldsink, Jonas S Fischer ... Liesbeth M Veenhoff
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Developmental Biology

    Sp Transcription Factors Establish the Signaling Environment in the Neuromesodermal Progenitor Niche During Axial Elongation

    Ravindra B Chalamalasetty, Haley Tran ... Terry P Yamaguchi
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Microbiology and Infectious Disease

    Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial species

    Letong Xu, Shenghai Shen ... Xin Deng
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Neuroscience

    Prenatal light exposure affects number sense and the mental number line in young domestic chicks

    Rosa Rugani, Matteo Macchinizzi ... Lucia Regolin
    In ovo light exposure that induces brain lateralization boosts chicks' number sense and spatial-numerical association.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    The lipocone superfamily, a unifying theme in metabolism of lipids, peptidoglycan and exopolysaccharides, inter-organismal conflicts and immunity

    A Maxwell Burroughs, Gianlucca G Nicastro, L Aravind
    A hitherto unrecognized superfamily of enzymes identified here provides unifying insights spanning lipid metabolism, anti-viral and anti-bacterial immunity, and the origin of developmental regulators like Wnt.
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development

    Pramila Rijal, Leiyan Wei ... Alain RM Townsend
    Grafting surface loops containing antibody epitopes of a poorly expressed flu neuraminidase protein onto a high-expressing, stable scaffold improves its production and stability for vaccine development.
    1. Evolutionary Biology

    Genetic parallels in biomineralization of the calcareous sponge Sycon ciliatum and stony corals

    Oliver Voigt, Magdalena V Wilde ... Gert Wörheide
    Discovery of calcarins in calcareous sponges highlights gene duplication and neofunctionalization as drivers of parallel biomineralization, offering insight into the independent evolution of calcification in early-diverging animals.
    1. Immunology and Inflammation
    2. Neuroscience

    Glia-mediated gut–brain cytokine signaling couples sleep to intestinal inflammatory responses induced by oxidative stress

    Alina Malita, Anne H Skakkebaek ... Kim Rewitz
    Gut-to-brain communication via glial cytokine signaling links intestinal oxidative stress to altered sleep patterns, highlighting how gut inflammation can influence brain function and restorative sleep behaviors essential for mental well-being.
    1. Neuroscience
    2. Stem Cells and Regenerative Medicine

    Endothelin B receptor inhibition rescues aging-dependent neuronal regenerative decline

    Rui Feng, Sarah F Rosen ... Valeria Cavalli
    Inhibition of the endothelin B receptor (ETBR) with FDA-approved Bosentan enhances axon regeneration and reverses age-related decline, revealing a promising therapeutic target for improving nerve repair in older individuals.
    1. Neuroscience

    Neural Connectome of the Ctenophore Statocyst

    Kei Jokura, Sanja Jasek ... Gáspár Jékely
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Neuroscience

    Morphology and ultrastructure of pharyngeal sense organs of Drosophila larvae

    Vincent Richter, Tilman Triphan ... Andreas S Thum
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    • Exceptional
    1. Chromosomes and Gene Expression
    2. Physics of Living Systems

    Criticality-driven enhancer-promoter dynamics in Drosophila chromosomes

    Gautham Ganesh, Jean-Bernard Fiche ... Julien Mozziconacci
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    From cognition to compensation: Neurocomputational mechanisms of guilt-driven and shame-driven altruistic behavior

    Ruida Zhu, Huanqing Wang ... Chao Liu
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology

    SLC4A1 mutations that cause distal renal tubular acidosis alter cytoplasmic pH and cellular autophagy

    Grace Essuman, Midhat Rizvi ... Emmanuelle Cordat
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Atomistic simulation of voltage activation of a truncated BK channel

    Zhiguang Jia, Jianhan Chen
    Ten-microsecond atomistic simulations directly capture the voltage activation of the Core-MT big potassium channel, revealing novel voltage sensing and sensor-pore coupling mechanisms that have largely eluded the community.
    1. Immunology and Inflammation

    Aurora kinase A promotes trained immunity via regulation of endogenous S-adenosylmethionine metabolism

    Mengyun Li, Huan Jin ... Xiaojun Xia
    The kinase activity of Aurora kinase A is required for preserving S-adenosylmethionine availability for histone methylation and epigenetic reprogramming during trained immunity induction in macrophages.
    1. Neuroscience

    Preparatory attentional templates in prefrontal and sensory cortex encode target-associated information

    Zhiheng Zhou, Joy Geng
    fMRI decoding reveals that the inferior frontal junction-visual network reinstates target-related information as a proxy for target localization before visual search.
    1. Structural Biology and Molecular Biophysics

    Sld3CBD–Cdc45 structural insights into Cdc45 recruitment for CMG complex formation during DNA replication

    Hao Li, Izumi Ishizaki ... Min Yao
    The crystal structure of Sld3CBD-Cdc45 shows its intermolecular interactions, and subsequent Sld3CBD-CMG model construction and binding assays enable a proposal for the mechanism of Cdc45 recruitment and Sld3 release.
    1. Neuroscience

    RubyACRs Enable Red-Shifted Optogenetic Inhibition in Freely Behaving Drosophila

    Daniel Bushey, Hiroshi Shiozaki ... Glenn C Turner
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Common psychiatric treatments alter affective dynamics

    Quentin Dercon, Quentin JM Huys ... Camilla L Nord
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Microbiology and Infectious Disease

    Mycobacterial Metallophosphatase MmpE Acts as a Nucleomodulin to Regulate Host Gene Expression and Promote Intracellular Survival

    Liu Chen, Baojie Duan ... Aizhen Guo
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    Pathogenic O-GlcNAc dyshomeostasis associated with cortical malformations and hyperactivity

    Florence Authier, Asad Jan ... Daan MF van Aalten
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    The Mycobacterium tuberculosis complex pangenome is small and shaped by sub-lineage-specific regions of difference

    Mahboobeh Behruznia, Maximillian Marin ... Conor J Meehan
    The accessory genome of Mycobacterium tuberculosis is small, but differences in gene content are found both between and within lineages.
    1. Developmental Biology

    Arrayed single-gene perturbations identify drivers of human anterior neural tube closure

    Roya E Huang, Giridhar M Anand ... Sharad Ramanathan
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Exceptional
    • Solid
    1. Neuroscience

    Structural and functional evidence supports re-defining mouse higher order visual areas into a single area V2

    Declan P Rowley, Madineh Sedigh-Sarvestani
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Decision-making components and times revealed by the single-trial electro-encephalogram

    Gabriel Weindel, Jelmer P Borst, Leendert van Maanen
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Microbiology and Infectious Disease

    Spatial integration of sensory input and motor output in Pseudomonas aeruginosa chemotaxis through colocalized distribution

    Zhengyu Wu, Maojin Tian ... Junhua Yuan
    In Pseudomonas aeruginosa, the chemoreceptor cluster and flagellar motor colocalize at the same cell pole through FlhF anchoring, preventing cross-pathway signaling interference between chemotaxis and c-di-GMP regulation.
    1. Immunology and Inflammation

    Hematopoietic stem and progenitor cells as a reservoir for trained immunity

    Brandon T Tran, Vidthiya Jeyanathan ... Katherine Y King
    Inflammation supports selection, differentiation bias, and epigenetic reprogramming of hematopoietic stem and progenitor cells to generate innate immune memory.
    1. Evolutionary Biology
    2. Immunology and Inflammation

    Newly discovered and conserved role of IgM against viral infection in an early vertebrate

    Weiguang Kong, Xinyou Wang ... Zhen Xu
    sIgM plays a role in viral neutralization across both primitive and modern vertebrates by following conserved principles in the development of specialized antiviral immunity.
    1. Evolutionary Biology

    Meaning-making behavior in a small-brained hominin, Homo naledi, from the late Pleistocene: contexts and evolutionary implications

    Agustín Fuentes, Marc Kissel ... Lee R Berger
    Evidence for H. naledi and increasing recognition of meaning-making behavior across the later Pleistocene suggests that the hominin emotional, socio-cognitive niche is broader and more significant than previously thought.
    1. Neuroscience

    Modality-Agnostic Decoding of Vision and Language from fMRI

    Mitja Nikolaus, Milad Mozafari ... Rullen VanRullen
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    A Forebrain Hub for Cautious Actions via the Midbrain

    Ji Zhou, Muhammad S Sajid ... Manuel A Castro-Alamancos
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    • Incomplete
    1. Microbiology and Infectious Disease
    2. Epidemiology and Global Health

    Inferring variant-specific effective reproduction numbers from combined case and sequencing data

    Marlin D Figgins, Trevor Bedford
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    iGABASnFR2: Improved genetically encoded protein sensors of GABA

    Ilya Kolb, Jeremy P Hasseman ... Glenn C Turner
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Neuroscience

    Kilohertz transcranial magnetic perturbation (kTMP) as a new non-invasive method to modulate cortical excitability

    Ludovica Labruna, Christina Merrick ... Daniel Sheltraw
    kTMP is a novel, non-invasive brain stimulation method that, via continuous magnetic induction, safely and effectively increases cortical excitability without any tactile sensation.
    1. Cell Biology

    Deletion of sulfate transporter SUL1 extends yeast replicative lifespan via reduced PKA signaling instead of decreased sulfate uptake

    Juan Long, Meng Ma ... Jing Yang
    SUL1 deletion extends lifespan via PKA-MSN2 pathways, underscoring nutrient signaling over sulfate transport in aging regulation.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    DNA methylation insulates genic regions from CTCF loops near nuclear speckles

    Shelby A Roseman, Allison P Siegenfeld ... Brian B Liau
    CTCF binding promotes chromatin-nuclear speckle interactions and gene activation after DNA demethylation, yet its binding and looping patterns are unaffected by speckle depletion.
    1. Genetics and Genomics

    WRN and WRNIP1 ATPases impose high fidelity on translesion synthesis by Y-family DNA polymerases

    Jung Hoon Yoon, Karthi Sellamuthu ... Satya Prakash
    WRN and WRNP1 ATPases together with WRN 3’ to 5’ exonuclease raise the fidelity of intrinsically highly error-prone Y-family DNA polymerases such that they perform error-free translesion synthesis.
    1. Plant Biology

    An increase of NPY1 expression leads to inhibitory phosphorylation of PIN-FORMED (PIN) proteins and suppression of pinoid (pid) null mutants

    Michael Mudgett, Zhouxin Shen ... Yunde Zhao
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    Listening to the room: disrupting activity of dorsolateral prefrontal cortex impairs learning of room acoustics in human listeners

    Heivet Hernández-Pérez, Jessica JM Monaghan ... David McAlpine
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Mood computational mechanisms underlying increased risk behavior in adolescent suicidal patients

    Zhihao Wang, Tian Nan ... Bastien Blain
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Immunology and Inflammation

    Toll-like receptors (TLRs) in the trained immunity era

    Lena Alexopoulou, Magali Irla
    Toll-like receptors (TLRs) shape trained immunity, balancing infection responses and chronic inflammation, and TLR agonists are promising immunomodulators for infectious diseases and cancer.
    1. Computational and Systems Biology

    The Product neutrality function defining genetic interactions emerges from mechanistic models of cell growth

    Lucas Fuentes Valenzuela, Paul Francois, Jan M Skotheim
    Coarse-grained models of cellular growth and high-throughput empirical genetic data support the Product neutrality function in which a double-mutant fitness is the product of single-mutant fitnesses.
    1. Neuroscience

    Acquisition phase-specific contribution of climbing fiber transmission to cerebellum-dependent motor memory in mice

    Jewoo Seo, Seung Ha Kim ... Sang Jeong Kim
    Climbing fiber activity gates the acquisition phase of motor learning but becomes nonessential for memory consolidation or performance once proficiency is achieved.
    Version of Record
    Short Report
    • Valuable
    • Incomplete
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Opening and closing of a cryptic pocket in VP35 toggles it between two different RNA-binding modes

    Upasana L Mallimadugula, Matthew A Cruz ... Gregory R Bowman
    The cryptic pocket is critical for one of the VP35 protein's functions and therefore is likely under selective pressure.
    1. Genetics and Genomics

    Gene expression variation across genetically identical individuals predicts reproductive traits

    Amy K Webster, John H Willis ... Patrick C Phillips
    Differences among genetically identical individuals can be explained using expression levels of just a handful of genes, suggesting that environmental variation can be functionally analyzed.
    1. Microbiology and Infectious Disease

    Bacteria detect neutrophils via a system that responds to hypochlorous acid and flow

    Ilona P Foik, Runhang Shu ... Albert Siryaporn
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience
    2. Computational and Systems Biology

    Morphogenesis and morphometry of brain folding patterns across species

    Sifan Yin, Chunzi Liu ... L Mahadevan
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Individual differences in fear memory expression engage distinct functional brain networks

    Barbara D Fontana, Jacob Hudock ... Justin W Kenney
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    • Incomplete
    1. Developmental Biology
    2. Neuroscience

    Biophysical basis for brain folding and misfolding patterns in ferrets and humans

    Gary PT Choi, Chunzi Liu ... L Mahadevan
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Biochemistry and Chemical Biology

    Development of D-box peptides to inhibit the anaphase-promoting complex/cyclosome

    Rohan Eapen, Cynthia Okoye ... Laura S Itzhaki
    Peptide inhibitors of E3 ubiquitin ligase anaphase-promoting complex/cyclosome, engineered for enhanced affinity by incorporating unnatural amino acids, are able to block substrate ubiquitination and drive degradation of a fused target.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Coral anthozoan-specific opsins employ a novel chloride counterion for spectral tuning

    Yusuke Sakai, Saumik Sen ... Akihisa Terakita
    Environmental chloride ions act as essential counterions that enable coral opsins to respond to visible light, and the polar residue Glu292 plays a critical role in chloride binding.
    1. Evolutionary Biology

    Evidence for deliberate burial of the dead by Homo naledi

    Lee R Berger, Tebogo Vincent Makhubela ... John Hawks
    Remains of the extinct hominin species Homo naledi were interred by members of their own species, the first time that burial has been documented in populations other than modern humans and Neanderthals.
    1. Neuroscience

    Automatic and accurate reconstruction of long-range axonal projections of single-neuron in mouse brain

    Lin Cai, Taiyu Fan ... Shaoqun Zeng
    A novel point assignment method achieves 80% f1-score in single-neuron reconstruction across broad brain regions, enabling high-throughput brain-wide projection mapping.

Magazine

  1. Episode 100: September 2025

    1. Cancer Biology

    Transcription Factor-Dependent Cancers: Targeting chromatin remodeling complexes to treat uveal melanoma

    Jonas A Nilsson
    1. Structural Biology and Molecular Biophysics

    Enzyme Dynamics: Capturing enzymes in motion

    Álvaro de la Gándara, Agnieszka A Kendrick

  4. Living Science: My grandmother’s rug

    Eve Marder
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Wnt Signaling: Exploring the origins of a signaling pathway

    Balaji Santhanam
    1. Developmental Biology

    Morphogenesis: Regulating closure of the neural tube in humans

    Leon Qarawani, Jacob H Hanna
    1. Neuroscience

    Numerical Cognition: Is ‘number sense’ a sense?

    Toshiya Matsushima
    1. Neuroscience

    Dorsal Vagal Complex: A cell atlas to guide research into appetite control

    Greta Lommi, Thomas A Lutz

  9. Point of View: How the Global South is reshaping scholarly communication

    Humberto Debat, Izuchukwu Azuka Okafor ... Carolina Santacruz-Perez