TY - JOUR TI - Functional genomic characterization of neoblast-like stem cells in larval Schistosoma mansoni AU - Wang, Bo AU - Collins, James J, III AU - Newmark, Phillip A A2 - Sánchez Alvarado, Alejandro VL - 2 PY - 2013 DA - 2013/07/30 SP - e00768 C1 - eLife 2013;2:e00768 DO - 10.7554/eLife.00768 UR - https://doi.org/10.7554/eLife.00768 AB - Schistosomes infect hundreds of millions of people in the developing world. Transmission of these parasites relies on a stem cell-driven, clonal expansion of larvae inside a molluscan intermediate host. How this novel asexual reproductive strategy relates to current models of stem cell maintenance and germline specification is unclear. Here, we demonstrate that this proliferative larval cell population (germinal cells) shares some molecular signatures with stem cells from diverse organisms, in particular neoblasts of planarians (free-living relatives of schistosomes). We identify two distinct germinal cell lineages that differ in their proliferation kinetics and expression of a nanos ortholog. We show that a vasa/PL10 homolog is required for proliferation and maintenance of both populations, whereas argonaute2 and a fibroblast growth factor receptor-encoding gene are required only for nanos-negative cells. Our results suggest that an ancient stem cell-based developmental program may have enabled the evolution of the complex life cycle of parasitic flatworms. KW - Schistosoma mansoni KW - stem cell KW - neoblast KW - trematode development KW - schistosomiasis JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -