TY - JOUR TI - Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant AU - Izumo, Mariko AU - Pejchal, Martina AU - Schook, Andrew C AU - Lange, Ryan P AU - Walisser, Jacqueline A AU - Sato, Takashi R AU - Wang, Xiaozhong AU - Bradfield, Christopher A AU - Takahashi, Joseph S A2 - Ptáček, Louis VL - 3 PY - 2014 DA - 2014/12/19 SP - e04617 C1 - eLife 2014;3:e04617 DO - 10.7554/eLife.04617 UR - https://doi.org/10.7554/eLife.04617 AB - In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% deletion of BMAL1 in the SCN and exhibited an immediate and complete loss of circadian behavior in constant conditions. Circadian rhythms in peripheral tissues persisted but became desynchronized and damped in constant darkness. The loss of synchrony was rescued by light/dark cycles and partially by restricted feeding (only in the liver and kidney but not in the other tissues) in a distinct manner. These results suggest that the forebrain/SCN is essential for internal temporal order of robust circadian programs in peripheral clocks, and that individual peripheral clocks are affected differently by light and feeding in the absence of a functional oscillator in the forebrain. KW - circadian rhythm KW - Bmal1 KW - Cre/loxP KW - suprachiasmatic nucleus KW - peripheral clock KW - light entrainment KW - Arntl KW - Mop3 JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -