TY - JOUR TI - Dynamic subcellular localization of a respiratory complex controls bacterial respiration AU - Alberge, François AU - Espinosa, Leon AU - Seduk, Farida AU - Sylvi, Léa AU - Toci, René AU - Walburger, Anne AU - Magalon, Axel A2 - Storz, Gisela VL - 4 PY - 2015 DA - 2015/06/16 SP - e05357 C1 - eLife 2015;4:e05357 DO - 10.7554/eLife.05357 UR - https://doi.org/10.7554/eLife.05357 AB - Respiration, an essential process for most organisms, has to optimally respond to changes in the metabolic demand or the environmental conditions. The branched character of their respiratory chains allows bacteria to do so by providing a great metabolic and regulatory flexibility. Here, we show that the native localization of the nitrate reductase, a major respiratory complex under anaerobiosis in Escherichia coli, is submitted to tight spatiotemporal regulation in response to metabolic conditions via a mechanism using the transmembrane proton gradient as a cue for polar localization. These dynamics are critical for controlling the activity of nitrate reductase, as the formation of polar assemblies potentiates the electron flux through the complex. Thus, dynamic subcellular localization emerges as a critical factor in the control of respiration in bacteria. KW - respiration KW - nitrate reductase KW - protonmotive force JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -