TY - JOUR TI - Structural dynamics of myosin 5 during processive motion revealed by interferometric scattering microscopy AU - Andrecka, Joanna AU - Ortega Arroyo, Jaime AU - Takagi, Yasuharu AU - de Wit, Gabrielle AU - Fineberg, Adam AU - MacKinnon, Lachlan AU - Young, Gavin AU - Sellers, James R AU - Kukura, Philipp A2 - Ha, Taekjip VL - 4 PY - 2015 DA - 2015/03/06 SP - e05413 C1 - eLife 2015;4:e05413 DO - 10.7554/eLife.05413 UR - https://doi.org/10.7554/eLife.05413 AB - Myosin 5a is a dual-headed molecular motor that transports cargo along actin filaments. By following the motion of individual heads with interferometric scattering microscopy at nm spatial and ms temporal precision we found that the detached head occupies a loosely fixed position to one side of actin from which it rebinds in a controlled manner while executing a step. Improving the spatial precision to the sub-nm regime provided evidence for an ångstrom-level structural transition in the motor domain associated with the power stroke. Simultaneous tracking of both heads revealed that consecutive steps follow identical paths to the same side of actin in a compass-like spinning motion demonstrating a symmetrical walking pattern. These results visualize many of the critical unknown aspects of the stepping mechanism of myosin 5 including head–head coordination, the origin of lever-arm motion and the spatiotemporal dynamics of the translocating head during individual steps. KW - molecular motor KW - myosin KW - single particle tracking JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -