1. Immunology and Inflammation

Mitochondrial Ca2+ and membrane potential, an alternative pathway for Interleukin 6 to regulate CD4 cell effector function

  1. Rui Yang
  2. Dario Lirussi
  3. Tina M Thornton
  4. Dawn M Jelley-Gibbs
  5. Sean A Diehl
  6. Laure K Case
  7. Muniswamy Madesh
  8. Douglas J Taatjes
  9. Cory Teuscher
  10. Laura Haynes
  11. Mercedes Rincón  Is a corresponding author
  1. University of Vermont, United States
  2. Helmholtz Center for Infection Research, Germany
  3. Taconic, United States
  4. Temple University, United States
  5. Trudeau Institute, United States
https://doi.org/10.7554/eLife.06376
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  1. Rui Yang
  2. Dario Lirussi
  3. Tina M Thornton
  4. Dawn M Jelley-Gibbs
  5. Sean A Diehl
  6. Laure K Case
  7. Muniswamy Madesh
  8. Douglas J Taatjes
  9. Cory Teuscher
  10. Laura Haynes
  11. Mercedes Rincón
(2015)
Mitochondrial Ca2+ and membrane potential, an alternative pathway for Interleukin 6 to regulate CD4 cell effector function
eLife 4:e06376.
https://doi.org/10.7554/eLife.06376
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Abstract

IL-6 plays an important role in determining the fate of effector CD4 cells and the cytokines that these cells produce. Here we identify a novel molecular mechanism by which IL-6 regulates CD4 cell effector function. We show that IL-6-dependent signal facilitates the formation of mitochondrial respiratory chain supercomplexes to sustain high mitochondrial membrane potential late during activation of CD4 cells. Mitochondrial hyperpolarization caused by IL-6 is uncoupled from the production of ATP by oxidative phosphorylation. However, it is a mechanism to raise the levels of mitochondrial Ca2+ late during activation of CD4 cells. Increased levels of mitochondrial Ca2+ in the presence of IL-6 are used to prolong Il4 and Il21 expression in effector CD4 cells. Thus, the effect of IL-6 on mitochondrial membrane potential and mitochondrial Ca2+ is an alternative pathway by which IL-6 regulates effector function of CD4 cells and it could contribute to the pathogenesis of inflammatory diseases.

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Author details

  1. Rui Yang

    Department of Medicine, Immunobiology Program, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.

  2. Dario Lirussi

    Department of Vaccinology and Applied Microbiology, Helmholtz Center for Infection Research, Braunschweig, Germany
    Competing interests
    The authors declare that no competing interests exist.

  3. Tina M Thornton

    Department of Medicine, Immunobiology Program, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.

  4. Dawn M Jelley-Gibbs

    Taconic, Germantown, United States
    Competing interests
    The authors declare that no competing interests exist.

  5. Sean A Diehl

    Department of Medicine, Immunobiology Program, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.

  6. Laure K Case

    Department of Medicine, Immunobiology Program, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.

  7. Muniswamy Madesh

    Department of Medical Genetics and Molecular Biochemistry, Temple University, Philadelphia, United States
    Competing interests
    The authors declare that no competing interests exist.

  8. Douglas J Taatjes

    Department of Pathology and Laboratory Medicine, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.

  9. Cory Teuscher

    Department of Medicine, Immunobiology Program, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.

  10. Laura Haynes

    Trudeau Institute, Saranac lake, United States
    Competing interests
    The authors declare that no competing interests exist.

  11. Mercedes Rincón

    Department of Medicine, Immunobiology Program, University of Vermont, Burlington, United States
    For correspondence
    mrincon@uvm.edu
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Ruslan Medzhitov, Howard Hughes Medical Institute, Yale University School of Medicine, United States

Ethics

Animal experimentation: All procedures performed on the mice were approved by the Institutional Animal Care and Use Committee (IACUC) of University of Vermont using protocols #12-032 (Rincon), #11-024 (Teuscher) and by the IACUC of Trudeau Institute using protocol #03-005 (Haynes).

Version history

  1. Received: January 7, 2015
  2. Accepted: May 13, 2015
  3. Accepted Manuscript published: May 14, 2015 (version 1)
  4. Version of Record published: May 29, 2015 (version 2)

Copyright

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

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  1. Rui Yang
  2. Dario Lirussi
  3. Tina M Thornton
  4. Dawn M Jelley-Gibbs
  5. Sean A Diehl
  6. Laure K Case
  7. Muniswamy Madesh
  8. Douglas J Taatjes
  9. Cory Teuscher
  10. Laura Haynes
  11. Mercedes Rincón
(2015)
Mitochondrial Ca2+ and membrane potential, an alternative pathway for Interleukin 6 to regulate CD4 cell effector function
eLife 4:e06376.
https://doi.org/10.7554/eLife.06376

Share this article

https://doi.org/10.7554/eLife.06376

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