TY - JOUR TI - Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo AU - Benson, Robert A AU - MacLeod, Megan KL AU - Hale, Benjamin G AU - Patakas, Agapitos AU - Garside, Paul AU - Brewer, James M A2 - Sakaguchi, Shimon VL - 4 PY - 2015 DA - 2015/08/10 SP - e06994 C1 - eLife 2015;4:e06994 DO - 10.7554/eLife.06994 UR - https://doi.org/10.7554/eLife.06994 AB - The production of high affinity, class switched antibodies produced by B cells hinges on the effective differentiation of T follicular helper (Tfh) cells. Here we define conditions specifically enhancing Tfh differentiation and providing protection in a model of influenza infection. Tfh responses were associated with prolonged antigen presentation by dendritic cells (DCs), which maintained T cell/DC interactions into stage 3 (>72 hr) of activation. Blocking stage 3 interactions ablated Tfh generation, demonstrating a causal link between T cell-DC behaviour and functional outcomes. The current data therefore explain how duration of antigen presentation affects the dynamics of T cell-DC interactions and consequently determine Tfh cell differentiation in the developing immune response. KW - T follicular helper KW - multiphoton KW - antigen presentation KW - antibody KW - influenza vaccine JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -