TY - JOUR TI - Plasma membrane overgrowth causes fibrotic collagen accumulation and immune activation in Drosophila adipocytes AU - Zang, Yiran AU - Wan, Ming AU - Liu, Min AU - Ke, Hongmei AU - Ma, Shuangchun AU - Liu, Lu-Ping AU - Ni, Jian-Quan AU - Carlos Pastor-Pareja, José A2 - Lemaître, Bruno VL - 4 PY - 2015 DA - 2015/06/19 SP - e07187 C1 - eLife 2015;4:e07187 DO - 10.7554/eLife.07187 UR - https://doi.org/10.7554/eLife.07187 AB - Many chronic diseases are associated with fibrotic deposition of Collagen and other matrix proteins. Little is known about the factors that determine preferential onset of fibrosis in particular tissues. Here we show that plasma membrane (PM) overgrowth causes pericellular Collagen accumulation in Drosophila adipocytes. We found that loss of Dynamin and other endocytic components causes pericellular trapping of outgoing Collagen IV due to dramatic cortex expansion when endocytic removal of PM is prevented. Deposits also form in the absence of negative Toll immune regulator Cactus, excess PM being caused in this case by increased secretion. Finally, we show that trimeric Collagen accumulation, downstream of Toll or endocytic defects, activates a tissue damage response. Our work indicates that traffic imbalances and PM topology may contribute to fibrosis. It also places fibrotic deposits both downstream and upstream of immune signaling, consistent with the chronic character of fibrotic diseases. KW - extracellular matrix KW - basement membrane KW - membrane transport JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -