Resection is responsible for loss of transcription around a double-strand break in Saccharomyces cerevisiae
Abstract
Emerging evidence indicate that the mammalian checkpoint kinase ATM induces transcriptional silencing in cis to DNA double-strand breaks (DSBs) through a poorly understood mechanism. Here we show that in Saccharomyces cerevisiae a single DSB causes transcriptional inhibition of proximal genes independently of Tel1/ATM and Mec1/ATR. Since the DSB ends undergo nucleolytic degradation (resection) of their 5′-ending strands, we investigated the contribution of resection in this DSB-induced transcriptional inhibition. We discovered that resection-defective mutants fail to stop transcription around a DSB, and the extent of this failure correlates with the severity of the resection defect. Furthermore, Rad9 and generation of γH2A reduce this DSB-induced transcriptional inhibition by counteracting DSB resection. Therefore, the conversion of the DSB ends from double-stranded to single-stranded DNA, which is necessary to initiate DSB repair by homologous recombination, is responsible for loss of transcription around a DSB in S. cerevisiae.
https://doi.org/10.7554/eLife.08942.001eLife digest
DNA is constantly under assault from harmful chemicals; some of which are produced inside the cell, while others come from outside of the cell. Breaks that form across both strands in a DNA double helix are considered the most dangerous type of DNA damage, and can cause a cell to die or become cancerous if they are not repaired accurately.
‘Homologous recombination’ is one of the main mechanisms used by cells to repair DNA double-strand breaks. This mechanism requires enzymes to eat away at the end of one of the DNA strands on each side of the double-strand break. This process is called ‘resection’ and it exposes single strands of DNA. These single-stranded DNA ‘tails’ are then free to interact with an intact copy of the same DNA sequence from elsewhere in the cell's nucleus, which is used as a guide when repairing the damage.
The proteins involved in homologous recombination have to work around other processes that go on inside the nucleus, such as the transcription of DNA in genes into RNA molecules. Previous research has reported that forming a double-strand break in the DNA reduces the levels of transcription for the genes that surround the break, but it was not clear how this occurred.
In mammalian cells, inhibiting the transcription of genes around a double-strand DNA break depends on a signaling pathway that is activated whenever DNA damage is detected. Manfrini et al. now show that this is not the case for budding yeast (Saccharomyces cerevisiae). Instead, the experiments indicate that it is the resection of the DNA around a double-strand break to form single-stranded tails that inhibits transcription in budding yeast. One of the next challenges will be to see if the resection process makes any contribution to changes in the transcription of genes that surround a double-strand break in mammals as well.
https://doi.org/10.7554/eLife.08942.002Introduction
DNA double-strand breaks (DSBs) are particularly dangerous for cells, since their inefficient or inaccurate repair can result in deletions and chromosomal translocations that can lead to cancer and/or severe developmental abnormalities in humans. DSB formation leads to activation of a complex DNA damage response (DDR), whose key players are highly conserved protein kinases, which include human ATM and ATR, as well as their Saccharomyces cerevisiae orthologs Tel1 and Mec1 (Gobbini et al., 2013). Once activated by DSBs, ATM/Tel1 and ATR/Mec1 promote DSB repair, delay cell cycle progression or trigger the elimination of genetically unstable cells by inducing cell death.
One of the main mechanisms to repair DSBs is homologous recombination (HR), which requires resection of the broken ends in order to generate 3′-ended single-stranded DNA (ssDNA) tails that invade the homologous undamaged template. In S. cerevisiae, DSB resection is initiated by the evolutionarily conserved MRX (Mre11-Rad50-Xrs2) complex that, together with Sae2, catalyzes the initial processing of the DSB ends. The 5′-ending strands can then be further degraded by two other machineries depending on Exo1 and Sgs1-Dna2, respectively (Symington and Gautier, 2011).
In eukaryotes, the DDR proteins function in the context of a highly organized chromatin environment that needs to be overcome to gain access to damaged DNA. Histone modifications and ATP-dependent chromatin remodelling proteins help to overcome this barrier by altering chromatin structure at the site of damage (Price and D'Andrea, 2013). One of the most characterized histone modifications is the phosphorylation of histone H2AX by ATM/Tel1 and ATR/Mec1, which spreads away from the DSB into large domains of surrounding chromatin (Rogakou et al., 1999; Downs et al., 2000; Burma et al., 2001; Ward and Chen, 2001). Other chromatin alterations detected at DSBs are associated with open and decondensed chromatin, indicating that chromatin at DSBs undergoes a transition to a more open, less compact conformation (Price and D'Andrea, 2013).
However, several proteins associated with repressive or transcriptionally inactive chromatin, including HP1, PcG (Polycomb group) proteins, PRMD2 methyltransferase, KAP-1, su(var)3-9 methyltrasferase variant (SUV3-9) and the macrohistone variant macroH2A1, are recruited to DNA lesions (Soria et al., 2012), suggesting that repressive chromatin can be generated around DSBs. Consistent with this hypothesis, generation of several DSBs distal to the promoter of a reporter gene in mammalian cells leads to ATM-dependent transcriptional repression of this reporter gene (Shanbhag et al., 2010), possibly through phosphorylation of the transcriptional elongation factor ENL (Ui et al., 2015). Similarly, RNA polymerase I-mediated transcription of rDNA is inhibited in an ATM-dependent manner in the vicinity of DSBs (Kruhlak et al., 2007). Furthermore, the steady state RNA levels of genes proximal to a single DSB have been observed to decrease by microarray analysis also in S. cerevisiae cells (Lee et al., 2000).
However, a different study in mammalian cells, where individual DSBs were induced at discrete endogenous sequences, showed that only transcription of the DSB-containing gene was affected (Pankotai et al., 2012). Furthermore, non-coding RNAs that control DDR activation are induced in the surrounding of DSBs in both vertebrates and Arabidopsis (Francia et al., 2012; Michalik et al., 2012; Wei et al., 2012), indicating that transcription can occur around DSBs.
Given these apparently contrasting results, other studies are required to understand how DSBs affect transcription in their surroundings. Furthermore, as DSBs are resected to generate 3′-ended ssDNA, and ATM, macroH2A1, PRMD2 and HP1 proteins are required for this process (Jazayeri et al., 2006; Soria and Almouzni, 2013; Ayrapetov et al., 2014; Khurana et al., 2014), whether DSB resection has a role in the DSB-induced transcriptional inhibition needs to be investigated.
By using the budding yeast HO endonuclease to create single DSBs at different chromosomal loci, we show that DSB induction causes Mec1- and Tel1-independent transcriptional inhibition of genes surrounding the DSB site. Failure to resect the DSB ends prevents this transcriptional inhibition, which is instead enhanced by accelerating the resection process. Altogether, these data indicate that loss of transcription around a DSB in S. cerevisiae cells is due to the conversion of DSB ends from double-stranded DNA (dsDNA) to ssDNA.
Results
Transcription is reduced in cis to a DSB at the MAT locus
To investigate changes in the transcription of genes surrounding DSBs, we took advantage of a yeast haploid strain (JKM139) where a single DSB can be generated at the MAT locus by galactose-induced expression of the HO endonuclease. As the homologous donor sequences HML and HMR are deleted in this strain, this DSB cannot be repaired by HR (Lee et al., 1998). We previously used this strain for total RNA-seq analysis of protein-coding gene expression upon induction of the HO-induced DSB to show that mRNA levels of the vast majority of protein-coding genes underwent no significant change upon DSB generation (Manfrini et al., 2015). Here we focused on the MAT locus that was not analyzed in the previous study. We mapped the RNA-seq data from two biological replicates of the JKM139 wild-type strain grown in raffinose (time zero, T0) and shifted to galactose-containing medium for 60 (T60) and 240 (T240) min on a restricted reference ‘genome’ corresponding to the MAT locus ±10 kb (Figure 1A). Quantitative analysis of tag densities for annotated genes in this region revealed a moderate signal decrease for the DSB-proximal genes 60 min after HO induction, and a strong signal reduction along the whole region after 240 min (Figure 1B). Strikingly, after 240 min of HO induction, density for the proximal genes BUD5, HMRA2, HMRA1 and TAF2 showed a ≥10-fold decrease compared to time zero, while density was only reduced by about twofold for the distal genes SNT1, IMG1 and BUD23 (Figure 1C).

Decrease of RNA levels around a DNA double-strand break (DSB).
(A) Strand-specific RNA-seq data from the two biological replicates of wild type strain (JKM139) before (time zero, T0) and 60 (T60) or 240 (T240) min after HO induction, were uniquely mapped to the MAT locus ±10 kb. For each time point, densities (tag/nucleotide, log2 scale) from the two replicates were pooled and visualized as a heatmap with the upper and lower panels corresponding to the + and − strands, respectively. Black arrows represent annotated genes. (B) Density for genes along the MAT locus ±10 kb at T0, T60 and T240 after HO induction. Mean values ±s.d. were calculated from the two biological replicates analyzed. RRT12 showed too low signal to be significantly quantified and was not included. (C) Ratio of density for genes along the MAT locus ±10 kb at T0, T60 and T240 after HO induction. For each time point, densities were normalized on the values obtained at T0. Mean values ±s.d. were calculated as above. RNA-seq data used to construct the graphs of Figure 1B,C are available in Figure 1—source data 1.
-
Figure 1—source data 1
RNA-seq data used to construct the graphs of Figure 1B,C.
- https://doi.org/10.7554/eLife.08942.004
Validation of the above data by using quantitative reverse transcriptase PCR (qRT-PCR) confirmed the reduction in transcript levels for genes located around the HO-induced DSB 240 min after HO induction (Figure 2A). The level of these mRNAs decreased progressively as the distance of the corresponding genes from the DSB diminished and such decrease was independent of the strand that was transcribed (Figure 2A). These decreases measured 240 min after HO induction were not influenced by mRNA stability, as all the analyzed mRNAs have half-lives shorter than 50 min (Geisberg et al., 2014).

DSB-induced transcriptional inhibition at the MAT locus.
(A) YEPR exponentially growing cell cultures of the JKM139 strain, carrying the HO cut site at the MAT locus, were transferred to YEPRG at T0 to induce HO. RNA levels of genes located at different distances from the HO cut site were evaluated by quantitative reverse transcriptase PCR (qRT-PCR) at T0 and T240 after HO induction. Results are presented as ratios between T240 and T0. RNA levels were quantified using ∆∆Ct method and quantities were normalized to ACT1 RNA levels. The mean values ±s.d. are represented (n = 3). (B) Exponentially growing YEPR cell cultures of the JKM139 derivative strains expressing either a fully functional Rpb2-HA fusion protein or untagged Rpb2 were transferred to YEPRG at T0 to induce HO. Binding of Rpb2-HA at different distance from the DSB at T0 and T240 after HO induction was evaluated by ChIP and qPCR. Primers used were the same as in (A). Results are presented as ratios between Rpb2-HA and untagged Rpb2, both of which normalized against the corresponding input, at T240 relative to T0. The mean values ±s.d. are represented (n = 3). Rpb2-HA binding at the ACT1 gene was used as internal control.
Because both qRT-PCR and RNA-seq measured the steady state level of total RNAs, which is a balance between synthesis and degradation, we investigated whether the reduction of RNA levels for genes around the DSB was due to transcriptional inhibition. To this end, the binding of the Rpb2 second largest subunit of RNA polymerase II was measured at T0 and 240 min after HO induction at different distances from the DSB. Indeed, Rpb2 association in the surroundings of the DSB decreased and this effect progressively diminished as a function of the distance from the DSB (Figure 2B), indicating that transcription was inhibited specifically around the DSB.
Local transcriptional inhibition is a general response to DSB formation
To investigate whether the DSB-induced transcriptional inhibition was specific for the MAT locus, we analyzed RNA levels and RNA polymerase II binding in tGI354 and YFP17 strains, which carried the recognition site for the HO endonuclease at the ARG5,6 locus on chromosome V or at the LEU2 locus on chromosome III, respectively. Since tGI354 strain can use the uncleavable MATa-inc sequence on chromosome III as a donor to repair the HO-induced DSB by Rad51-dependent HR, this strain carried the deletion of RAD51. After generation of the HO-induced DSB at the ARG5,6 locus, we found that both the RNA levels of genes located in the surrounding of the DSB (Figure 3A) and Rbp2 occupancy (Figure 3B) progressively decreased as the distance of the corresponding genes from the DSB diminished. Similar results were obtained when the HO-induced DSB was generated at the LEU2 locus (Figure 3C,D). We conclude that inhibition of local transcription is a general response to DSB formation.

DSB-induced transcriptional inhibition at different chromosomal loci.
(A) YEPR exponentially growing cell cultures of tGI354 strain, carrying the HO site at the ARG5,6 locus and the deletion of RAD51 gene, were transferred to YEPRG at T0 to induce HO. RNA levels of genes located at different distances from the HO cut site were evaluated by qRT-PCR at T0 and T240 after HO induction, as described in Figure 2A. Results are presented as ratios between T240 and T0. The mean values ±s.d. are represented (n = 3). (B) Binding of Rpb2-HA from samples collected in (A) was evaluated by ChIP and qPCR as described in Figure 2B. Primers used were the same as in (A). The mean values ±s.d. are represented (n = 3). (C) YEPR exponentially growing cell cultures of YFP17 strain, carrying the HO cut site at the LEU2 locus, were transferred to YEPRG at T0 to induce HO. RNA levels were analyzed as in Figure 2A but normalized to the ALG9 gene transcript. Only transcription of genes located on the right side of the HO-induced DSB was analyzed because no transcription units are present on the left side of the break. The mean values ±s.d. are represented (n = 3). (D) Binding of Rpb2-HA from samples collected in (C) was evaluated by ChIP and qPCR as described in Figure 2B. Primers used were the same as in (C).
The checkpoint kinases Tel1 and Mec1 are not required for DSB-induced transcriptional inhibition
In all eukaryotes, DNA DSBs induce a DDR that depends on the checkpoint kinases ATM/Tel1 and ATR/Mec1 (Gobbini et al., 2013). As transcriptional repression in proximity to DSBs requires ATM in mammalian cells (Kruhlak et al., 2007; http://journal.frontiersin.org/Journal/10.3389/fgene.2013.00136/full, Shanbhag et al., 2010), we asked whether Tel1 and/or Mec1 were necessary for transcriptional silencing of genes flanking the HO-induced DSB. HO expression was induced by galactose addition in mec1∆, tel1∆ and mec1∆ tel1∆ cells (mec1∆ and mec1∆ tel1∆ cells were kept viable by SML1 deletion) and mRNA levels were analyzed 240 min after HO induction. As the lack of Mec1 causes cells to progress through the cell cycle even after DSB formation, HO was induced in cells arrested in G2 with nocodazole and kept arrested in G2 during HO-cut induction. As shown in Figure 4, the amount of RNA transcribed from genes surrounding the DSB was reduced to quite similar extents in HO-induced wild type, mec1∆ sml1∆, tel1∆ and mec1∆ tel1∆ sml1∆ cells, with mec1∆ sml1∆ cells showing a more pronounced decrease. This finding indicates that neither Tel1 nor Mec1 are responsible for DSB-induced transcriptional inhibition.

DSB-induced transcriptional inhibition does not require Mec1 and Tel1.
YEPR exponentially growing cell cultures of the JKM139 strain, carrying the HO cut site at the MAT locus, were arrested in G2 with nocodazole and transferred at T0 to YEPRG in the presence of nocodazole. RNA levels were analyzed by qRT-PCR as described in Figure 2A. The mean values ±s.d. are represented (n = 3).
γH2A and Rad9 limit DSB-induced transcriptional inhibition by negatively regulating DSB resection
A single DSB triggers phosphorylation of histone H2A to form γH2A, which spreads on each side of the DSB to about 50 kb distance (Rogakou et al., 1999; Shroff et al., 2004). Interestingly, γH2A diminishes strongly over highly transcribed regions and H2A phosphorylation is rapidly restored if transcription is subsequently inhibited (Lee et al., 2014). We therefore investigated whether γH2A plays a role in DSB-induced transcriptional inhibition by expressing HO in hta1-S129A cells, which produced a H2A variant carrying a non-phosphorylatable alanine residue replacing Ser129. As histone H2A is encoded by the two genes HTA1 and HTA2, hta1-S129A cells also carried the HTA2 deletion. Strikingly, not only RNA levels of genes proximal to the DSB still decreased in hta1-S129A cells, but this decrease was more severe than in wild type cells (Figure 5A), indicating that γH2A counteracts repression of transcription around the DSB.

The lack of γH2A enhances transcriptional inhibition around the DSB by accelerating resection.
(A) YEPR exponentially growing cell cultures of the JKM139 derivative strains, carrying the HO cut site at the MAT locus, were transferred to YEPRG at T0. RNA levels of genes located in the surroundings of the HO cut site at the MAT locus were analyzed at T0 and T240 after HO induction by qRT-PCR as described in Figure 2A. The mean values ±s.d. are represented (n = 3). (B) Method to measure DSB resection. Gel blots of SspI-digested genomic DNA separated on alkaline agarose gel were hybridized with a single-stranded MAT probe (ss probe) that anneals to the unresected strand. 5′–3′ resection progressively eliminates SspI sites (S), producing larger SspI fragments (r1 through r7) detected by the probe. (C) DSB resection. Genomic DNA prepared from samples collected in (A) was analysed for single-stranded DNA (ssDNA) formation at the indicated times after HO induction as described in (B). The image that was used for the cropped final Figure 5C is available in Figure 5—source data 1.
-
Figure 5—source data 1
Image that was used for the cropped final Figure 5C.
- https://doi.org/10.7554/eLife.08942.009
The ends of DSBs are nucleolytically processed to generate 3′-ended ssDNA that initiate HR. DSB resection is negatively regulated by the checkpoint protein Rad9, which inhibits mainly the Sgs1-Dna2 resection machinery (Bonetti et al., 2015; Ferrari et al., 2015). The lack of γH2A reduces the amount of Rad9 bound at the DSB ends and, as a consequence, hta1-S129A cells resect the DSB ends more efficiently than wild type cells (Eapen et al., 2012; Clerici et al., 2014). Thus, we investigated whether the enhanced DSB-induced transcriptional repression observed in hta1-S129A mutant cells was due to their accelerated nucleolytic processing of the DSB ends. Resection of the DSB 5′ strand can be measured by following the loss of restriction fragments by Southern blot analysis with a ssRNA probe annealing on one side of the break (Figure 5B). Strikingly, SGS1 deletion, which reduced the accelerated resection in hta1-S129A cells to almost wild type levels (Figure 5C), also reduced their enhanced DSB-induced transcriptional inhibition (Figure 5A). Furthermore, rad9∆ cells, which undergo accelerated DSB resection (Lazzaro et al., 2008), showed transcription inhibition around the DSB to the same extent as hta1-S129A cells (Figure 5A). Altogether these data indicate that γH2A and Rad9 limit the inhibition of transcription around the DSB ends by counteracting the resection process.
Loss of transcription around a DSB depends on resection
We then investigated if generation of ssDNA was responsible for transcription inhibition at DSBs. Resection has been reported to initiate asynchronously from the ends of DSBs (Shroff et al., 2004) and to move about 4 kb/hr (Vaze et al., 2002). After 60 min of HO induction, wild type cells accumulated mostly r2 resection products, indicating that DSB resection in most cells had not proceeded beyond 3.5 kb from the DSB (Figure 6A). At the same time point, we detected a strong decrease of Rpb2 occupancy at the TAF2 and BUD5 genes, which are located within 4 kb from either side of the DSB (Figure 6B). Concomitantly with the progression of 5′–3′ resection (Figure 6A), also the binding of Rpb2 to distal genes progressively diminished (Figure 6B), suggesting that the decrease of RNA polymerase II occupancy around the DSB correlates with the time it takes for a DNA-end to become single-stranded.

Resection mutants fail to reduce transcription around the DSB.
(A) DSB resection. YEPR exponentially growing cell cultures of JKM139 derivative strains, carrying the HO cut site at the MAT locus, were transferred to YEPRG at T0. Formation of ssDNA was determined as described in Figure 5B. (B) Binding of Rpb2-HA in wild type cells from samples collected in (A) was evaluated by ChIP and qPCR as described in Figure 2B. The mean values ±s.d. are represented (n = 3). (C) Binding of Rpb2-HA in exo1∆ sgs1∆ cells from samples collected in (A) was evaluated by ChIP and qPCR as described in Figure 2B. The mean values ±s.d. are represented (n = 3). (D) RNA levels from samples collected in (A) were analyzed at T0 and T240 after HO induction by qRT-PCR as described in Figure 2A. The mean values ±s.d. are represented (n = 3). Contiguous images that were used for the cropped final Figure 6A are available in Figure 6—source data 1.
-
Figure 6—source data 1
Contiguous images that were used for the cropped final Figure 6A.
- https://doi.org/10.7554/eLife.08942.011
DSB resection in S. cerevisiae is initiated by MRX and Sae2, which catalyze an endonucleolytic cleavage of the 5′ strands. The resulting partially resected 5′ strand can be further processed by the nucleases Exo1 and Dna2, the latter working in concert with the 3′–5′ helicase Sgs1 (Mimitou and Symington, 2008; Zhu et al., 2008). DSB resection is severely affected by either simultaneous inactivation of Exo1 and Sgs1 or the lack of MRX, with the latter not only providing the endonuclease activity to initiate resection, but also promoting the loading of Exo1, Sgs1 and Dna2 at DSB ends (Shim et al., 2010). To assess whether DSB resection has a role in the DSB-induced transcriptional silencing, we asked whether exo1∆, mre11∆ or exo1∆ sgs1∆ cells, which were impaired in resection to different extents, were still capable to repress transcription of genes in the vicinity of the HO-induced DSB. Indeed, neither the amount of Rpb2 bound around the HO-induced DSB (Figure 6C) nor RNA levels were decreased in exo1∆ sgs1∆ cells (Figure 6D), which had the most severe DSB resection defect compared to mre11∆ and exo1∆ single mutants (Figure 6A). RNA levels only slightly decreased in HO-induced mre11∆ cells (Figure 6D), which were severely defective in the accumulation of resection products (Figure 6A), while only the RNA levels of genes proximal to the break site were diminished in exo1∆ mutant cells (Figure 6D), which initiated DSB resection but failed to accumulate resection products longer than 3.5 kb (Figure 6A). Thus, loss of transcription around a DSB in S. cerevisiae is due to the conversion of dsDNA to ssDNA by the resection machinery.
Discussion
Here, we further extend the observation that induction of a DSB at the MAT locus in S. cerevisiae cells leads to a decrease of the RNA steady state levels of proximal genes (Lee et al., 2000), by demonstrating that this decrease is not locus specific and is due to dissociation of RNA polymerase II from its template DNA. In contrast to the reported requirement of mammalian ATM for transcription silencing around a DSB (Kruhlak et al., 2007; Shanbhag et al., 2010; Ui et al., 2015), neither Tel1 nor Mec1 appear to be necessary for transcriptional inhibition at the DSB site in S. cerevisiae. Instead, we found that mutants defective in DSB resection fail to inhibit transcription around a DSB, and the extent of this failure correlates with the severity of the DSB resection defect. Furthermore, accelerating DSB resection by preventing γH2A generation or by eliminating Rad9 enhances this DSB-induced transcriptional inhibition. Altogether, these data indicate that loss of transcription around a DSB in S. cerevisiae is not due to an active regulatory mechanism, but to the conversion of the DSB ends from dsDNA to ssDNA.
Nucleolytic processing of the template DNA strand should stop transcription of the corresponding gene. However, we found that mRNA levels and RNA polymerase II occupancy around a DSB decrease independently of the strand that is transcribed, indicating that loss of non-template DNA strands also impedes transcription. Therefore, the most likely reason of why the conversion from dsDNA to ssDNA stops transcription is that the transcription machinery binds only dsDNA. Consistent with this hypothesis, it has been shown that within a region containing a stretch of dsDNA preceding a single-strand 3′ DNA end, purified RNA polymerase II transcribes within the dsDNA portion (Lilley and Houghton, 1979; Kadesch and Chamberlin, 1982). Furthermore, structural analyses of transcription initiation reveals that RNA polymerase II and its associated General Transcription Factors bind double-stranded promoter DNA to form a closed preinitiation complex (PIC), where the transcriptional machinery interacts with both template and non-template DNA strands (Liu et al., 2013; Sainsbury et al., 2015). Moreover, removal of either the template or non-template strands prevents binding of T7 RNA polymerase to the promoter, supporting the importance of protein-dsDNA interaction in the spatial organization of the transcriptional initiation complex (Maslak and Martin, 1993).
Interaction of the transcriptional machinery with dsDNA also allows transcription initiation and elongation. In fact, the double-stranded promoter DNA follows a straight path in the PIC complex and this rigidity allows the subsequent transition from a closed to an open promoter complex, where a central DNA region is melted leading to a transcription bubble in which the DNA template strand enters the RNA polymerase II cleft (Murakami et al., 2013). Furthermore, competition between the non-template DNA strand and the RNA transcript for base-pairing with the DNA template strand was shown to be important for maintaining structure and function during elongation (Kireeva et al., 2011), reinforcing the inhibitory effects of ssDNA on the transcription process.
In summary, while DSB-induced transcriptional repression in mammals is reportedly an active mechanism controlled by ATM, the stop of transcription around a DSB in S. cerevisiae cells is due to the conversion of dsDNA to ssDNA that is necessary to initiate DSB repair by HR (Figure 7). In any case, while Mec1 and Tel1 are not required to resect a DSB, ATM and the chromatin compaction promoting proteins macroH2A1, PRMD2 and HP1 promote end resection by facilitating the loading of BRCA1 at the DSB ends (Soria and Almouzni, 2013; Ayrapetov et al., 2014; Khurana et al., 2014). Thus, whether the nucleolytic processing of the DSB ends contributes to repress transcription around a DSB also in mammals is an important question that remains to be addressed. In fact, although in human cells inactivation of the end resection factor CtIP does not restore trascription around the DSB (Shanbhag et al., 2010), other resection machineries are known to be active in resecting DSBs in CtIP-depleted cells (Tomimatsu et al., 2012) and could contribute to inhibit transcription around the DSB.

Model for loss of transcription around a DSB.
Resection of the 5′ strands at both DSB ends leads to release of the transcription machinery (dashed lines) and to subsequent transcription arrest independently of whether the degraded DNA strand acts as template or non-template. Since the RNA polymerase binds double-stranded promoter DNA, generation of ssDNA at the DSB ends prevents reinitiation events (bar-headed line). Blue arrows indicate direction of transcription.
Materials and methods
Yeast strains
Request a detailed protocolThe yeast strains used in this study are derivatives of JKM139, YFP17 or tGI354 strains and are listed in Supplementary file 1. Cells were grown in YEP medium (1% yeast extract, 2% peptone) supplemented with 2% glucose (YEPD), 2% raffinose (YEPR) or 2% raffinose and 3% galactose (YEPRG).
DSB resection
Request a detailed protocolDSB end resection at the MAT locus in JKM139 derivative strains was analyzed on alkaline agarose gels as previously described (Clerici et al., 2008), by using a single-stranded probe complementary to the unresected DSB strand. This probe was obtained by in vitro transcription using Promega (Madison, WI) Riboprobe System-T7 and plasmid pML514 as a template. Plasmid pML514 was constructed by inserting in the pGEM7Zf EcoRI site a 900-bp fragment containing part of the MATα locus (coordinates 200870 to 201587 on chromosome III).
Total RNA-seq analysis
Request a detailed protocolTotal RNA-seq libraries were previously described (Manfrini et al., 2015) and data were retrieved from the Gene Expression Omnibus (accession number GSE63444; Manfrini et al., 2014). Reads were uniquely mapped as described (Manfrini et al., 2015) on a reference ‘genome’ corresponding to the MATa sequence ±10 kb, that was manually reconstructed by replacing MATα-specific elements with the corresponding MATa-specific elements in the MATα locus sequence ±10 kb (chr. 3, positions 188671 to 211177) retrieved from SGD (http://www.yeastgenome.org/). Data were normalized using the normalization factors previously used for the whole transcriptome analysis (Manfrini et al., 2015), based on the total number of reads that mapped on all the ORFs of the yeast genome.
qRT-PCR
Request a detailed protocolTotal RNA was extracted from cells using the Bio-Rad (Hercules, CA) Aurum total RNA mini kit. First strand cDNA synthesis was performed with the Bio-Rad iScript cDNA Synthesis Kit. qRT-PCR was performed on a MiniOpticon Real-time PCR system (Bio-Rad) and RNA levels were quantified using the ∆∆Ct method. Quantities were normalized to either ACT1 or ALG9 RNA levels. Since RNR1 is transcriptionally induced immediately after HO-induced DSB, its RNA level at T240 was normalized on the value obtained 30 min after HO induction, when DSB resection was not proceeded beyond 1.7 kb. Primer sequences are provided in Supplementary file 2.
ChIP analysis
Request a detailed protocolChIP analysis was performed as previously described (Viscardi et al., 2007). Chromatin extracts from both RPB2-HA and RPB2 strains were immunoprecipitated with anti-HA antibodies (12CA5). Input and immunoprecipitated DNA were purified and analyzed by qPCR. Amplicons were chosen well within the coding region of the genes and of the highly transcribed ACT1 gene on chromosome VI as a control. The ratio between values obtained for Rpb2-HA and those obtained with the untagged Rpb2 immunoprecipitated samples, both of which normalized against the corresponding input, was calculated for each time point after HO induction. The obtained values were divided by the ratio calculated from uninduced cells, arbitrarily set to 1, for each amplicon. Primer sequences are provided in Supplementary file 2.
Data availability
-
RNA processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNAPublicly available at the NCBI Gene Expression Omnibus (Accession no: GSE63444).
References
-
DNA double-strand breaks promote methylation of histone H3 on lysine 9 and transient formation of repressive chromatinProceedings of the National Academy of Sciences of USA 111:9169–9174.https://doi.org/10.1073/pnas.1403565111
-
ATM phosphorylates histone H2AX in response to DNA double-strand breaksThe Journal of Biological Chemistry 276:42462–42467.https://doi.org/10.1074/jbc.C100466200
-
The Saccharomyces cerevisiae chromatin remodeler Fun30 regulates DNA end resection and checkpoint deactivationMolecular and Cellular Biology 32:4727–4740.https://doi.org/10.1128/MCB.00566-12
-
Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA templateThe Journal of Biological Chemistry 257:5286–5295.
-
Interaction of RNA polymerase II fork loop 2 with downstream non-template DNA regulates transcription elongationThe Journal of Biological Chemistry 286:30898–30910.https://doi.org/10.1074/jbc.M111.260844
-
Dynamics of yeast histone H2A and H2B phosphorylation in response to a double-strand breakNature Structural & Molecular Biology 21:103–109.https://doi.org/10.1038/nsmb.2737
-
Arrest, adaptation, and recovery following a chromosome double-strand break in Saccharomyces cerevisiaeCold Spring Harbor Symposia on Quantitative Biology 65:303–314.https://doi.org/10.1101/sqb.2000.65.303
-
The interaction of RNA polymerase II from wheat with supercoiled and linear plasmid templatesNucleic Acids Research 6:507–523.https://doi.org/10.1093/nar/6.2.507
-
RNA polymerase II transcription: structure and mechanismBiochimica et Biophysica Acta 1829:2–8.https://doi.org/10.1016/j.bbagrm.2012.09.003
-
RNA processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNARNA processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA, NCBI Gene Expression Omnibus, GSE63444, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE63444.
-
A small RNA response at DNA ends in DrosophilaNucleic Acids Research 40:9596–9603.https://doi.org/10.1093/nar/gks711
-
DNAPKcs-dependent arrest of RNA polymerase II transcription in the presence of DNA breaksNature Structural & Molecular Biology 19:276–282.https://doi.org/10.1038/nsmb.2224
-
Megabase chromatin domains involved in DNA double-strand breaks in vivoThe Journal of Cell Biology 146:905–916.https://doi.org/10.1083/jcb.146.5.905
-
Structural basis of transcription initiation by RNA polymerase IINature Reviews. Molecular Cell Biology 16:129–143.https://doi.org/10.1038/nrm3952
-
Double-strand break end resection and repair pathway choiceAnnual Review of Genetics 45:247–271.https://doi.org/10.1146/annurev-genet-110410-132435
-
MRX-dependent DNA damage response to short telomeresMolecular Biology of the Cell 18:3047–3058.https://doi.org/10.1091/mbc.E07-03-0285
-
Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stressThe Journal of Biological Chemistry 276:47759–47762.https://doi.org/10.1074/jbc.C100569200
Decision letter
-
Jessica K TylerReviewing Editor; University of Texas MD Anderson Cancer Center, United States
eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.
Thank you for submitting your work entitled “Resection drives transcriptional repression around a DNA double-strand break in Saccharomyces cerevisiae” for peer review at eLife. Your submission has been favorably evaluated by Jim Kadonaga (Senior Editor) and three reviewers, one of whom is a member of our Board of Reviewing Editors.
The reviewers have discussed the reviews with one another, and the Reviewing Editor has drafted this decision to help you prepare a revised submission.
Summary:
We all felt that this was an important and high quality study that clearly supported the novel finding that transcription stops due to DNA resection.
1) We all felt that the likely reason why transcription stops and RNA polymerase II occupancy is lost upon resection is because the transcription machinery, such as TBP and RNA polymerase II bind to dsDNA and not ssDNA. This stopping transcription due to loss of DNA template model is quite a different model from the current repression model, which implies an active regulatory mechanism. As such, an in depth discussion of loss of transcription as a consequence of loss of DNA template with appropriate citations, and a model figure to illustrate this idea, is needed.
2) The data generated from the HO site at LEU2 and the HO site at MAT need to be in two separate figures, and the difference emphasized more strongly in the text. Otherwise this will all be assumed to be at MAT and the apparent novelty of this work, compared to the previous Lee et al. paper, will be reduced.
3) The result with the mec1∆ tel1∆ double mutant needs to be reported in order to conclude that the DNA damage checkpoint is not required for stopping transcription around a DNA double strand break.
Reviewer #1:
Although brief, this is an important study that makes significant advances on the previous work from Jim Haber's lab showing that there is progressive transcriptional repression around the DNA double strand break (DSB) induced by the HO endonuclease at the yeast MAT locus. There are two important novel results from the current work: 1) the transcriptional repression around a DSB is not dependent on checkpoint activation (in contrast to the situation in mammalian cells); 2) transcriptional repression is dependent on DNA resection. This last finding is very novel and exciting, and has not yet been examined in other systems. The work is of high quality, but the following points need to be addressed:
1) Given the partial redundancy of Tel1 and Mec1 in activation of the DNA damage checkpoint, it is important that they show that transcriptional repression around the HO site is not affected in a tel1 mec1 sml1 mutant, in order to make the conclusion that the activation of the DNA damage checkpoint is not required for transcriptional repression.
2) Mechanically, it makes sense that DNA resection would block transcription, given that RNA polymerase II binds to double strand DNA, and cannot transcribe from single strand DNA. This should be discussed briefly.
Reviewer #2:
This is a very straightforward analysis of a consequence of DNA double-strand break on local transcription. There are a plethora of observations in the literature that bare on this issue, motivating the authors to carry out these studies. At the risk of oversimplification, the message from this story seems to be very simply simplified, that conversion of DNA from double strand to single strand, that allows homologous recombination, has an ‘unintended’ consequence; genes that become single-stranded can no longer by transcribed (presumably it’s well know that RNA polymerase binding and regulation etc. occurs only on double-strand templates, and not on single-strand templates). All of their lovely experiments are consistent with this simple idea, an idea they only state once and, in my view, deserves to be highlighted in the Abstract, and supported with a model at manuscript’s end. Sometimes biology does have simple explanations; this seems to be one of those times.
I have but a few issues I think important to address, most in the writing. The experiments appear impeccable, thorough, clearly illustrated.
1) They need to emphasize that they carried out this analysis on a non-MAT locus DSB, Figure 2D is of an HO break at the LEU2 locus. So, they have tested 2 DSBs. Is their general conclusion made any more convincing by test of a third DSB? Yet, showing data at the HO site at LEU2 is critical for the generality of their conclusions. I suggest they therefore put the LEU2 HO data in a separate Figure. Shape the data in a similar fashion as the HO data, state distance from HO site, extending left and right from the DSB, as they did for the MAT HO site data. Parallel presentations make it easier for the reader.
2) As I said in the general comments, they need to show a model. The ‘take home’ message is simple, and easily grasped by all with a model. That model can have Rad9 and H2A∆ in it. Yet, most critically is the double-strand to single-strand conversion as the mechanistic explanation. And this inhibition appears to be perhaps unintended consequences of a separate biological reaction, generating a substrate for recombination.
3) They need a citation that in fact single-strand templates do not support transcription.
4) I do not quite get how the transcriptional repression cannot be Mec1 regulated, if Mec1 regulates degradation by regulating Rad9, etc.? And degradation of telomere proximal DNA has complex interactions with Mec1 and Rad53, and Rad9; a word on how the telomere resection is or is not relevant here.
5) In the Results section, the authors allude to a previous paper (Manfrini et al., 2015, EMBO Rep) stating: “We previously used this strain for total RNA-seq analysis of protein-coding gene expression upon induction of the HO-induced DSB”. What did they conclude from that study relevant to this study?
6) The conclusion that the inhibition is not cell cycle regulated, based on but two experiments – asynchronous and G2/M synchronized – is underwhelming. At least under those two conditions they see no difference; tone down the generalization, as the experiments do not support that conclusion. I do not suggest getting rigorous about the cell cycle effects, or lack thereof; this is not an important issue, it seems. Just have the language match the data in this point.
7) Make it more transparent how gene transcription reduction correlates with extent of degradation. So, when reporting on the r2 restriction site, state how far away it is from the HO site, what gene is there, and how much that genes transcription is reduced and at what time.
8) At the Results outset, I think it best to set the reader up for the story and technical issues. It is relevant that the time scale, in hours, is far longer than the half-lives of the mRNAs involved, though is this known? Therefore, half-lives of mRNAs does not muddle the analysis.
9) In the Discussion section, you state that “DSB resection not only generates ssDNA ends necessary to initiate HR, but can also facilitate the access of DNA repair proteins to the site of damage by releasing the transcriptional machinery from the DSB. That is speculation, right? Label as such.
Reviewer #3:
In the manuscript by Manfrini et al, the authors examine the transcript levels of genes flanking a DNA break in budding yeast and find that transcript levels decrease over time. The decrease in transcription correlates with resection levels, and strains with altered kinetics of resection show corresponding altered kinetics of transcription changes.
The data in the manuscript are generally of good quality and the changes in transcription and resection are very clear. The really major problem with this manuscript is that the conclusions are written in such a way as to imply that resection is regulating transcriptional repression. However, the data in the manuscript very clearly demonstrates that at the 240 minute time point (at which most of the transcription assays are performed), there is extensive resection of the area containing the genes being analyzed. Resection of double-stranded DNA will prevent transcription factors (such as TBP) from binding to promoter elements and as a result, transcription is no longer possible (and of course, depending on the orientation of the gene, resection can mean that there is no longer a template for the RNA polymerase!). Consequently, most of the cells in the population are unable to carry out transcription of these genes at this time point. This is quite different from ‘transcriptional repression’, which implies regulation. At earlier time points (e.g. shown in Figure 1), the only genes affected are precisely those genes that would no longer be completely double stranded in many cells. The ChIP data in Figure 4 fully support this (i.e. that loss of the double stranded DNA sequence results in loss of binding by RNA polymerase II).
Therefore, what the manuscript is in fact demonstrating is that transcription is never repressed in yeast in genes flanking a DNA break. It is only impeded by loss of DNA sequence during resection. This is different from what has been established in the mammalian system, where there is an active mechanism for signalling to the transcriptional machinery over multiple kilobases where resection isn't taking place. Therefore, if the manuscript is rewritten to make this clear it would be of interest.
https://doi.org/10.7554/eLife.08942.015Author response
1) We all felt that the likely reason why transcription stops and RNA polymerase II occupancy is lost upon resection is because the transcription machinery, such as TBP and RNA polymerase II bind to dsDNA and not ssDNA. This stopping transcription due to loss of DNA template model is quite a different model from the current repression model, which implies an active regulatory mechanism. As such, an in depth discussion of loss of transcription as a consequence of loss of DNA template with appropriate citations, and a model figure to illustrate this idea, is needed.
We have now provided a discussion of loss of transcription as consequence of loss of DNA template with appropriate references. Furthermore, we now show a model in new Figure 7 to illustrate this idea.
2) The data generated from the HO site at LEU2 and the HO site at MAT need to be in two separate figures, and the difference emphasized more strongly in the text. Otherwise this will all be assumed to be at MAT and the apparent novelty of this work, compared to the previous Lee et al., paper, will be reduced.
We now show the data regarding the HO site at the MAT and LEU2 loci in two separate figures (Figure 2 and 3). To emphasize that DSB-induced transcriptional inhibition is not locus specific, we have analyzed transcription around a third DSB at the ARG5,6 locus (see point 1, reviewer 2). The data regarding loss of transcription around the DSB at the LEU2 and ARG5,6 loci are reported in the new Figure 3.
3) The result with the mec1∆ tel1∆ double mutant needs to be reported in order to conclude that the DNA damage checkpoint is not required for stopping transcription around a DNA double strand break.
We have repeated the analysis in mec1∆ tel1∆ double mutant. The double mutant represses transcription around the DSB like each single mec1∆ and tel1∆ mutant. These results are reported in new Figure 4.
Reviewer #1:
Although brief, this is an important study that makes significant advances on the previous work from Jim Haber's lab showing that there is progressive transcriptional repression around the DNA double strand break (DSB) induced by the HO endonuclease at the yeast MAT locus. There are two important novel results from the current work: 1) the transcriptional repression around a DSB is not dependent on checkpoint activation (in contrast to the situation in mammalian cells); 2) transcriptional repression is dependent on DNA resection. This last finding is very novel and exciting, and has not yet been examined in other systems. The work is of high quality, but the following point need to be addressed:
Given the partial redundancy of Tel1 and Mec1 in activation of the DNA damage checkpoint, it is important that they show that transcriptional repression around the HO site is not affected in a tel1 mec1 sml1 mutant, in order to make the conclusion that the activation of the DNA damage checkpoint is not required for transcriptional repression.
We have repeated the analysis in mec1∆ tel1∆ double mutant. The double mutant represses transcription around the DSB like each single mec1∆ and tel1∆ mutant. These results are reported in new Figure 4.
Mechanically, it makes sense that DNA resection would block transcription, given that RNA polymerase II binds to double strand DNA, and cannot transcribe from single strand DNA. This should be discussed briefly.
We have discussed this point.
Reviewer #2:
1) They need to emphasize that they carried out this analysis on a non-MAT locus DSB, Figure 2D is of an HO break at the LEU2 locus. So, they have tested 2 DSBs. Is their general conclusion made any more convincing by test of a third DSB? Yet, showing data at the HO site at LEU2 is critical for the generality of their conclusions. I suggest they therefore put the LEU2 HO data in a separate Figure. Shape the data in a similar fashion as the HO data, state distance from HO site, extending left and right from the DSB, as they did for the MAT HO site data. Parallel presentations make it easier for the reader.
We now show the data regarding the HO site at the MAT and LEU2 loci in two separate figures (new Figures 2 and 3). To emphasize that DSB-induced transcriptional inhibition is not locus specific, we have analyzed transcription around a third DSB at the ARG5,6 locus. The data regarding loss of transcription around the DSB at the LEU2 and ARG5,6 loci are reported in new Figure 3.
2) As I said in the general comments, they need to show a model. The ‘take home’ message is simple, and easily grasped by all with a model. That model can have Rad9 and H2A∆ in it. Yet, most critically is the double-strand to single-strand conversion as the mechanistic explanation. And this inhibition appears to be perhaps unintended consequences of a separate biological reaction, generating a substrate for recombination.
We now show a model in new Figure 7.
3) They need a citation that in fact single-strand templates do not support transcription.
We have now provided a discussion of loss of transcription as consequence of loss of DNA template with appropriate references. Furthermore, we now show a model in new Figure 7 to illustrate this idea.
4) I do not quite get how the transcriptional repression cannot be Mec1 regulated, if Mec1 regulates degradation by regulating Rad9, etc.? And degradation of telomere proximal DNA has complex interactions with Mec1 and Rad53, and Rad9; a word on how the telomere resection is or is not relevant here.
We have previously shown that cells lacking Mec1 accelerates DSB resection because they fail to recruit Rad9 to the DSB (Clerici et al., 2014). However, DSB resection in mec1 mutant cells is not as fast as in rad9 cells because Mec1 has also a positive role in promoting resection by phosphorylating and activating Sae2 (Clerici et al., 2014). In any case, consistent with a slight acceleration of DSB resection, the decrease in mRNA levels in mec1 mutant cells in figure 4 is slightly more pronounced than in wild type.
We prefer not discussing resection at telomeres, because it is quite limited compared to that at DNA DSBs and we do not know the impact on transcription.
5) In the Results section, the authors allude to a previous paper (Manfrini et al., 2015, EMBO Rep) stating: “We previously used this strain for total RNA-seq analysis of protein-coding gene expression upon induction of the HO-induced DSB”. What did they conclude from that study relevant to this study?
We have modified the sentence.
6) The conclusion that the inhibition is not cell cycle regulated, based on but two experiments – asynchronous and G2/M synchronized – is underwhelming. At least under those two conditions they see no difference; tone down the generalization, as the experiments do not support that conclusion. I do not suggest getting rigorous about the cell cycle effects, or lack thereof; this is not an important issue, it seems. Just have the language match the data in this point.
We have deleted the paragraph.
7) Make it more transparent how gene transcription reduction correlates with extent of degradation. So, when reporting on the r2 restriction site, state how far away it is from the HO site, what gene is there, and how much that genes transcription is reduced and at what time.
We have modified the text accordingly.
8) At the Results outset, I think it best to set the reader up for the story and technical issues. It is relevant that the time scale, in hours, is far longer than the half-lives of the mRNAs involved, though is this known? Therefore, half-lives of mRNAs does not muddle the analysis.
We have added a sentence that the RNA decreases at 240 minutes after HO induction were not influenced by mRNA stability, as all the analyzed mRNAs have half-lives shorter than 50 minutes (Geisberg et al., 2014).
9) In the Discussion section, you state that “DSB resection not only generates ssDNA ends necessary to initiate HR, but can also facilitate the access of DNA repair proteins to the site of damage by releasing the transcriptional machinery from the DSB. That is speculation, right? Label as such.
We have modified the sentence.
Reviewer #3:
[…] The data in the manuscript are generally of good quality and the changes in transcription and resection are very clear. The really major problem with this manuscript is that the conclusions are written in such a way as to imply that resection is regulating transcriptional repression. However, the data in the manuscript very clearly demonstrates that at the 240 minute time point (at which most of the transcription assays are performed), there is extensive resection of the area containing the genes being analyzed. Resection of double-stranded DNA will prevent transcription factors (such as TBP) from binding to promoter elements and as a result, transcription is no longer possible (and of course, depending on the orientation of the gene, resection can mean that there is no longer a template for the RNA polymerase!). Consequently, most of the cells in the population are unable to carry out transcription of these genes at this time point. This is quite different from ‘transcriptional repression’, which implies regulation. At earlier time points (e.g. shown in Figure 1), the only genes affected are precisely those genes that would no longer be completely double stranded in many cells. The ChIP data in Figure 4 fully support this (i.e. that loss of the double stranded DNA sequence results in loss of binding by RNA polymerase II).
Therefore, what the manuscript is in fact demonstrating is that transcription is never repressed in yeast in genes flanking a DNA break. It is only impeded by loss of DNA sequence during resection. This is different from what has been established in the mammalian system, where there is an active mechanism for signalling to the transcriptional machinery over multiple kilobases where resection isn't taking place. Therefore, if the manuscript is rewritten to make this clear it would be of interest.
We have modified the title and the text to make the point raised by the reviewer clear.
https://doi.org/10.7554/eLife.08942.016Article and author information
Author details
Funding
Associazione Italiana per la Ricerca sul Cancro (IG15210)
- Maria Pia Longhese
PRIN 2010-2011
- Maria Pia Longhese
Agence Nationale de la Recherche (REGULncRNA)
- Antonin Morillon
European Research Council (ERC) (EpincRNA Starting grant)
- Antonin Morillon
European Research Council (ERC) (DARK Consolidator grant)
- Antonin Morillon
Associazione Italiana per la Ricerca sul Cancro (IG12971)
- Fabrizio d'Adda di Fagagna
Human Frontier Science Program (HFSP) (RGP0014/2012)
- Fabrizio d'Adda di Fagagna
Fondazione Cariplo (2010.0818)
- Fabrizio d'Adda di Fagagna
Fondazione Telethon (GGP12059)
- Fabrizio d'Adda di Fagagna
PRIN 2010-2011
- Fabrizio d'Adda di Fagagna
Istituto Nazionale Genetica Molecolare (MIUR EPIGEN)
- Fabrizio d'Adda di Fagagna
European Research Council (ERC) (322728)
- Fabrizio d'Adda di Fagagna
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank J Haber and G Liberi for strains and G Lucchini for critical reading of the manuscript. High-throughput sequencing was performed by the NGS platform of Institut Curie, supported by the grants ANR-10-EQPX-03 and ANR10-INBS-09-08 from the Agence Nationale de la Recherche (investissements d'avenir) and by the Canceropôle Ile-de-France. NM was supported by a fellowship from Fondazione Italiana per la Ricerca sul Cancro (FIRC).
Reviewing Editor
- Jessica K Tyler, University of Texas MD Anderson Cancer Center, United States
Version history
- Received: May 22, 2015
- Accepted: July 30, 2015
- Accepted Manuscript published: July 31, 2015 (version 1)
- Version of Record published: August 19, 2015 (version 2)
Copyright
© 2015, Manfrini et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 2,307
- Page views
-
- 528
- Downloads
-
- 23
- Citations
Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.
Download links
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Further reading
-
- Cell Biology
- Chromosomes and Gene Expression
The cohesin complex plays essential roles in chromosome segregation, 3D genome organisation, and DNA damage repair through its ability to modify DNA topology. In higher eukaryotes, meiotic chromosome function, and therefore fertility, requires cohesin complexes containing meiosis-specific kleisin subunits: REC8 and RAD21L in mammals and REC-8 and COH-3/4 in Caenorhabditis elegans. How these complexes perform the multiple functions of cohesin during meiosis and whether this involves different modes of DNA binding or dynamic association with chromosomes is poorly understood. Combining time-resolved methods of protein removal with live imaging and exploiting the temporospatial organisation of the C. elegans germline, we show that REC-8 complexes provide sister chromatid cohesion (SCC) and DNA repair, while COH-3/4 complexes control higher-order chromosome structure. High-abundance COH-3/4 complexes associate dynamically with individual chromatids in a manner dependent on cohesin loading (SCC-2) and removal (WAPL-1) factors. In contrast, low-abundance REC-8 complexes associate stably with chromosomes, tethering sister chromatids from S-phase until the meiotic divisions. Our results reveal that kleisin identity determines the function of meiotic cohesin by controlling the mode and regulation of cohesin–DNA association, and are consistent with a model in which SCC and DNA looping are performed by variant cohesin complexes that coexist on chromosomes.
-
- Chromosomes and Gene Expression
- Developmental Biology
Though long non-coding RNAs (lncRNAs) represent a substantial fraction of the Pol II transcripts in multicellular animals, only a few have known functions. Here we report that the blocking activity of the Bithorax complex (BX-C) Fub-1 boundary is segmentally regulated by its own lncRNA. The Fub-1 boundary is located between the Ultrabithorax (Ubx) gene and the bxd/pbx regulatory domain, which is responsible for regulating Ubx expression in parasegment PS6/segment A1. Fub-1 consists of two hypersensitive sites, HS1 and HS2. HS1 is an insulator while HS2 functions primarily as an lncRNA promoter. To activate Ubx expression in PS6/A1, enhancers in the bxd/pbx domain must be able to bypass Fub-1 blocking activity. We show that the expression of the Fub-1 lncRNAs in PS6/A1 from the HS2 promoter inactivates Fub-1 insulating activity. Inactivation is due to read-through as the HS2 promoter must be directed toward HS1 to disrupt blocking.