TY - JOUR TI - Promoter nucleosome dynamics regulated by signalling through the CTD code AU - Materne, Philippe AU - Anandhakumar, Jayamani AU - Migeot, Valerie AU - Soriano, Ignacio AU - Yague-Sanz, Carlo AU - Hidalgo, Elena AU - Mignion, Carole AU - Quintales, Luis AU - Antequera, Francisco AU - Hermand, Damien A2 - Reinberg, Danny VL - 4 PY - 2015 DA - 2015/06/22 SP - e09008 C1 - eLife 2015;4:e09008 DO - 10.7554/eLife.09008 UR - https://doi.org/10.7554/eLife.09008 AB - The phosphorylation of the RNA polymerase II C-terminal domain (CTD) plays a key role in delineating transcribed regions within chromatin by recruiting histone methylases and deacetylases. Using genome-wide nucleosome mapping, we show that CTD S2 phosphorylation controls nucleosome dynamics in the promoter of a subset of 324 genes, including the regulators of cell differentiation ste11 and metabolic adaptation inv1. Mechanistic studies on these genes indicate that during gene activation a local increase of phospho-S2 CTD nearby the promoter impairs the phospho-S5 CTD-dependent recruitment of Set1 and the subsequent recruitment of specific HDACs, which leads to nucleosome depletion and efficient transcription. The early increase of phospho-S2 results from the phosphorylation of the CTD S2 kinase Lsk1 by MAP kinase in response to cellular signalling. The artificial tethering of the Lsk1 kinase at the ste11 promoter is sufficient to activate transcription. Therefore, signalling through the CTD code regulates promoter nucleosomes dynamics. KW - RNA polymerase KW - chromatin KW - Set1 KW - MAP kinase KW - HDAC JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -