TY - JOUR TI - Neuron-glia signaling in developing retina mediated by neurotransmitter spillover AU - Rosa, Juliana M AU - Bos, Rémi AU - Sack, Georgeann S AU - Fortuny, Cécile AU - Agarwal, Amit AU - Bergles, Dwight E AU - Flannery, John G AU - Feller, Marla B A2 - Barres, Ben VL - 4 PY - 2015 DA - 2015/08/14 SP - e09590 C1 - eLife 2015;4:e09590 DO - 10.7554/eLife.09590 UR - https://doi.org/10.7554/eLife.09590 AB - Neuron-glia interactions play a critical role in the maturation of neural circuits; however, little is known about the pathways that mediate their communication in the developing CNS. We investigated neuron-glia signaling in the developing retina, where we demonstrate that retinal waves reliably induce calcium transients in Müller glial cells (MCs). During cholinergic waves, MC calcium transients were blocked by muscarinic acetylcholine receptor antagonists, whereas during glutamatergic waves, MC calcium transients were inhibited by ionotropic glutamate receptor antagonists, indicating that the responsiveness of MCs changes to match the neurotransmitter used to support retinal waves. Using an optical glutamate sensor we show that the decline in MC calcium transients is caused by a reduction in the amount of glutamate reaching MCs. Together, these studies indicate that neurons and MCs exhibit correlated activity during a critical period of retinal maturation that is enabled by neurotransmitter spillover from retinal synapses. KW - iGluSnFR KW - GCaMP KW - retinal ganglion cell JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -