TY - JOUR TI - Genetic architecture of natural variation in cuticular hydrocarbon composition in Drosophila melanogaster AU - Dembeck, Lauren M AU - Böröczky, Katalin AU - Huang, Wen AU - Schal, Coby AU - Anholt, Robert R H AU - Mackay, Trudy F C A2 - Kliebenstein, Daniel J VL - 4 PY - 2015 DA - 2015/11/14 SP - e09861 C1 - eLife 2015;4:e09861 DO - 10.7554/eLife.09861 UR - https://doi.org/10.7554/eLife.09861 AB - Insect cuticular hydrocarbons (CHCs) prevent desiccation and serve as chemical signals that mediate social interactions. Drosophila melanogaster CHCs have been studied extensively, but the genetic basis for individual variation in CHC composition is largely unknown. We quantified variation in CHC profiles in the D. melanogaster Genetic Reference Panel (DGRP) and identified novel CHCs. We used principal component (PC) analysis to extract PCs that explain the majority of CHC variation and identified polymorphisms in or near 305 and 173 genes in females and males, respectively, associated with variation in these PCs. In addition, 17 DGRP lines contain the functional Desat2 allele characteristic of African and Caribbean D. melanogaster females (more 5,9-C27:2 and less 7,11-C27:2, female sex pheromone isomers). Disruption of expression of 24 candidate genes affected CHC composition in at least one sex. These genes are associated with fatty acid metabolism and represent mechanistic targets for individual variation in CHC composition. KW - genome wide associaiton study KW - Drosophila genetic reference Panel KW - cuticular lipids KW - African Drosophila KW - multivariate analysis JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -