TY - JOUR TI - Temporal transcriptomics suggest that twin-peaking genes reset the clock AU - Pembroke, William G AU - Babbs, Arran AU - Davies, Kay E AU - Ponting, Chris P AU - Oliver, Peter L A2 - Ptáček, Louis VL - 4 PY - 2015 DA - 2015/11/02 SP - e10518 C1 - eLife 2015;4:e10518 DO - 10.7554/eLife.10518 UR - https://doi.org/10.7554/eLife.10518 AB - The mammalian suprachiasmatic nucleus (SCN) drives daily rhythmic behavior and physiology, yet a detailed understanding of its coordinated transcriptional programmes is lacking. To reveal the finer details of circadian variation in the mammalian SCN transcriptome we combined laser-capture microdissection (LCM) and RNA-seq over a 24 hr light / dark cycle. We show that 7-times more genes exhibited a classic sinusoidal expression signature than previously observed in the SCN. Another group of 766 genes unexpectedly peaked twice, near both the start and end of the dark phase; this twin-peaking group is significantly enriched for synaptic transmission genes that are crucial for light-induced phase shifting of the circadian clock. 341 intergenic non-coding RNAs, together with novel exons of annotated protein-coding genes, including Cry1, also show specific circadian expression variation. Overall, our data provide an important chronobiological resource (www.wgpembroke.com/shiny/SCNseq/) and allow us to propose that transcriptional timing in the SCN is gating clock resetting mechanisms. KW - circadian rhythm KW - transcriptomic KW - non-coding RNA JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -