TY - JOUR TI - The kinetochore prevents centromere-proximal crossover recombination during meiosis AU - Vincenten, Nadine AU - Kuhl, Lisa-Marie AU - Lam, Isabel AU - Oke, Ashwini AU - Kerr, Alastair RW AU - Hochwagen, Andreas AU - Fung, Jennifer AU - Keeney, Scott AU - Vader, Gerben AU - Marston, Adèle L A2 - Lichten, Michael VL - 4 PY - 2015 DA - 2015/12/14 SP - e10850 C1 - eLife 2015;4:e10850 DO - 10.7554/eLife.10850 UR - https://doi.org/10.7554/eLife.10850 AB - During meiosis, crossover recombination is essential to link homologous chromosomes and drive faithful chromosome segregation. Crossover recombination is non-random across the genome, and centromere-proximal crossovers are associated with an increased risk of aneuploidy, including Trisomy 21 in humans. Here, we identify the conserved Ctf19/CCAN kinetochore sub-complex as a major factor that minimizes potentially deleterious centromere-proximal crossovers in budding yeast. We uncover multi-layered suppression of pericentromeric recombination by the Ctf19 complex, operating across distinct chromosomal distances. The Ctf19 complex prevents meiotic DNA break formation, the initiating event of recombination, proximal to the centromere. The Ctf19 complex independently drives the enrichment of cohesin throughout the broader pericentromere to suppress crossovers, but not DNA breaks. This non-canonical role of the kinetochore in defining a chromosome domain that is refractory to crossovers adds a new layer of functionality by which the kinetochore prevents the incidence of chromosome segregation errors that generate aneuploid gametes. KW - meiosis KW - kinetochore KW - DNA repair KW - recombination KW - chromosome segregation KW - DNA break formation JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -