TY - JOUR TI - Comparative analysis of viral RNA signatures on different RIG-I-like receptors AU - Sanchez David, Raul Y AU - Combredet, Chantal AU - Sismeiro, Odile AU - Dillies, Marie-Agnès AU - Jagla, Bernd AU - Coppée, Jean-Yves AU - Mura, Marie AU - Guerbois Galla, Mathilde AU - Despres, Philippe AU - Tangy, Frédéric AU - Komarova, Anastassia V A2 - Cao, Xuetao VL - 5 PY - 2016 DA - 2016/03/24 SP - e11275 C1 - eLife 2016;5:e11275 DO - 10.7554/eLife.11275 UR - https://doi.org/10.7554/eLife.11275 AB - The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during viral infection, we tagged RLRs (RIG-I, MDA5, LGP2) and applied tagged protein affinity purification followed by next-generation sequencing (NGS) of associated RNA molecules. Two viruses with negative- and positive-sense RNA genome were used: measles (MV) and chikungunya (CHIKV). NGS analysis revealed that distinct regions of MV genome were specifically recognized by distinct RLRs: RIG-I recognized defective interfering genomes, whereas MDA5 and LGP2 specifically bound MV nucleoprotein-coding region. During CHIKV infection, RIG-I associated specifically to the 3’ untranslated region of viral genome. This study provides the first comparative view of the viral RNA ligands for RIG-I, MDA5 and LGP2 in the presence of infection. KW - innate immunity KW - RIG-I-like receptors KW - virus-host interactions KW - PAMP KW - PRR KW - ribonucleoproteins JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -