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Correction: Registered report: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology

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Cite this article as: eLife 2015;4:e13015 doi: 10.7554/eLife.13015

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Khan I, Kerwin J, Owen K, Griner E, Reproducibility Project: Cancer Biology. 2015. Registered report: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology. eLife 4:e08245. doi: 10.7554/eLife.08245

Published 3 September 2015

After publication of the Registered report, but before experimental work relating to these changes had commenced, the following revisions have been made.

First, the timing from transfection to generation of lysate for the Western blot procedure described in Protocol 4 has been corrected from 24 hr to 48 hr.

Second, the statistical analysis and power calculations for Protocols 2 and 4 have been expanded to include two additional comparisons, one between siPTEN/PTENP1 and siPTEN, and one between siPTEN/PTENP1 and siPTENP1. This results in an increase in the number of replicates for Protocol 2.

Third, the source of the siRNA used for knockdown of PTEN and PTENP1 in Protocols 2, 3, and 4 has been corrected. The siPTEN Smartpool from Dharmacon, which targets PTEN and PTENP1, has been included and will be used instead of a mix of siPTEN and siPTENP1.

Finally, the Introduction has also been expanded to include an additional citation (Johnsson et al., 2013).

The article has now been corrected.

Article and author information

Author details

  1. Israr Khan

    Competing interests
    IK: Alamo Laboratories Inc. is a Science Exchange associated laboratory.
  2. John Kerwin

    Competing interests
    JK: Biotechnology Research and Education Program, University of Maryland is a Science Exchange associated laboratory.
  3. Kate Owen

    Competing interests
    No competing interests declared.
  4. Erin Griner

    Competing interests
    No competing interests declared.
  5. Reproducibility Project: Cancer Biology

    Competing interests
    RP:CB: EI, FT, JL, and NP are employed and holds shares in Science Exchange Inc.

Publication history

  1. Version of Record published: November 24, 2015 (version 1)

Copyright

© 2015, Khan et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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