TY - JOUR TI - Synthetic protein interactions reveal a functional map of the cell AU - Berry, Lisa K AU - Ólafsson, Guðjón AU - Ledesma-Fernández, Elena AU - Thorpe, Peter H A2 - Schekman, Randy VL - 5 PY - 2016 DA - 2016/04/21 SP - e13053 C1 - eLife 2016;5:e13053 DO - 10.7554/eLife.13053 UR - https://doi.org/10.7554/eLife.13053 AB - To understand the function of eukaryotic cells, it is critical to understand the role of protein-protein interactions and protein localization. Currently, we do not know the importance of global protein localization nor do we understand to what extent the cell is permissive for new protein associations – a key requirement for the evolution of new protein functions. To answer this question, we fused every protein in the yeast Saccharomyces cerevisiae with a partner from each of the major cellular compartments and quantitatively assessed the effects upon growth. This analysis reveals that cells have a remarkable and unanticipated tolerance for forced protein associations, even if these associations lead to a proportion of the protein moving compartments within the cell. Furthermore, the interactions that do perturb growth provide a functional map of spatial protein regulation, identifying key regulatory complexes for the normal homeostasis of eukaryotic cells. KW - GBP KW - localization KW - nanobody KW - GFP JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -