1. Structural Biology and Molecular Biophysics
  2. Cell Biology
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RNA Localization: Finding FISH in a small pond

  1. Calvin H Jan  Is a corresponding author
  1. Calico Life Sciences LLC, United States
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Cite this article as: eLife 2016;5:e16598 doi: 10.7554/eLife.16598
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The distribution of mRNAs in E. coli reflects how newly built proteins are processed.

Genetic instructions encoded in the DNA of the chromosome are copied, or transcribed, by enzymes called RNA polymerases (purple) into mRNAs (thin lines). These mRNA are then translated into proteins by ribosomes (depicted in gray). Moffitt et al. show that mRNAs that encode soluble proteins (orange) diffuse freely from the chromosome and are translated throughout the cytosol. Similarly, mRNAs that encode proteins that will be release into the periplasm or beyond (blue) are found throughout the cytosol. Membrane proteins (green) are insoluble in the cytosol and are instead translated at the inner membrane. The degradasome (yellow) is also found at the inner membrane and preferentially breaks down nearby mRNAs.

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