TY - JOUR TI - The ubiquitin-proteasome system regulates focal adhesions at the leading edge of migrating cells AU - Teckchandani, Anjali AU - Cooper, Jonathan A A2 - Davis, Roger J VL - 5 PY - 2016 DA - 2016/09/22 SP - e17440 C1 - eLife 2016;5:e17440 DO - 10.7554/eLife.17440 UR - https://doi.org/10.7554/eLife.17440 AB - Cell migration requires the cyclical assembly and disassembly of focal adhesions. Adhesion induces phosphorylation of focal adhesion proteins, including Cas (Crk-associated substrate/p130Cas/BCAR1). However, Cas phosphorylation stimulates adhesion turnover. This raises the question of how adhesion assembly occurs against opposition from phospho-Cas. Here we show that suppressor of cytokine signaling 6 (SOCS6) and Cullin 5, two components of the CRL5SOCS6 ubiquitin ligase, inhibit Cas-dependent focal adhesion turnover at the front but not rear of migrating epithelial cells. The front focal adhesions contain phospho-Cas which recruits SOCS6. If SOCS6 cannot access focal adhesions, or if cullins or the proteasome are inhibited, adhesion disassembly is stimulated. This suggests that the localized targeting of phospho-Cas within adhesions by CRL5SOCS6 and concurrent cullin and proteasome activity provide a negative feedback loop, ensuring that adhesion assembly predominates over disassembly at the leading edge. By this mechanism, ubiquitination provides a new level of spatio-temporal control over cell migration. KW - cell adhesion KW - cell signaling KW - ubiquitin JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -