TY - JOUR TI - Postprandial sleep mechanics in Drosophila AU - Murphy, Keith R AU - Deshpande, Sonali A AU - Yurgel, Maria E AU - Quinn, James P AU - Weissbach, Jennifer L AU - Keene, Alex C AU - Dawson-Scully, Ken AU - Huber, Robert AU - Tomchik, Seth M AU - Ja, William W A2 - Scott, Kristin VL - 5 PY - 2016 DA - 2016/11/22 SP - e19334 C1 - eLife 2016;5:e19334 DO - 10.7554/eLife.19334 UR - https://doi.org/10.7554/eLife.19334 AB - Food consumption is thought to induce sleepiness. However, little is known about how postprandial sleep is regulated. Here, we simultaneously measured sleep and food intake of individual flies and found a transient rise in sleep following meals. Depending on the amount consumed, the effect ranged from slightly arousing to strongly sleep inducing. Postprandial sleep was positively correlated with ingested volume, protein, and salt—but not sucrose—revealing meal property-specific regulation. Silencing of leucokinin receptor (Lkr) neurons specifically reduced sleep induced by protein consumption. Thermogenetic stimulation of leucokinin (Lk) neurons decreased whereas Lk downregulation by RNAi increased postprandial sleep, suggestive of an inhibitory connection in the Lk-Lkr circuit. We further identified a subset of non-leucokininergic cells proximal to Lkr neurons that rhythmically increased postprandial sleep when silenced, suggesting that these cells are cyclically gated inhibitory inputs to Lkr neurons. Together, these findings reveal the dynamic nature of postprandial sleep. KW - feeding KW - sleep KW - behavior KW - neurogenetics KW - nutrition JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -