A non canonical subtilase attenuates the transcriptional activation of defence responses in Arabidopsis thaliana
- LIPM, Université de Toulouse, INRA, CNRS, France
- Fédération de Recherche 3450, Plateforme Imagerie, Pôle de Biotechnologie Végétale, France
Abstract
Proteases play crucial physiological functions in all organisms by controlling the lifetime of proteins. Here, we identified an atypical protease of the subtilase family [SBT5.2(b)] that attenuates the transcriptional activation of plant defence independently of its protease activity. The SBT5.2 gene produces two distinct transcripts encoding a canonical secreted subtilase [SBT5.2(a)] and an intracellular protein [SBT5.2(b)]. Concomitant to SBT5.2(a) downregulation, SBT5.2(b) expression is induced after bacterial inoculation. SBT5.2(b) localizes to endosomes where it interacts with and retains the defence-related TF MYB30. Nuclear exclusion of MYB30 results in its reduced transcriptional activation and, thus, suppressed resistance. sbt5.2 mutants, with abolished SBT5.2(a) and SBT5.2(b) expression, display enhanced defence that is suppressed in a myb30 mutant background. Moreover, overexpression of SBT5.2(b), but not SBT5.2(a), in sbt5.2 plants reverts the phenotypes displayed by sbt5.2 mutants. Overall, we uncover a regulatory mode of the transcriptional activation of defence responses previously undescribed in eukaryotes.
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Funding
Institut National de la Recherche Agronomique and Agreenskills
- Irene Serrano
Freach Ministry of National Education and Research
- Pierre Buscaill
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2016, Serrano et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A non canonical subtilase attenuates the transcriptional activation of defence responses in Arabidopsis thaliana
eLife 5:e19755.
https://doi.org/10.7554/eLife.19755
