TY - JOUR TI - The RFamide receptor DMSR-1 regulates stress-induced sleep in C. elegans AU - Iannacone, Michael J AU - Beets, Isabel AU - Lopes, Lindsey E AU - Churgin, Matthew A AU - Fang-Yen, Christopher AU - Nelson, Matthew D AU - Schoofs, Liliane AU - Raizen, David M A2 - Marder, Eve VL - 6 PY - 2017 DA - 2017/01/17 SP - e19837 C1 - eLife 2017;6:e19837 DO - 10.7554/eLife.19837 UR - https://doi.org/10.7554/eLife.19837 AB - In response to environments that cause cellular stress, animals engage in sleep behavior that facilitates recovery from the stress. In Caenorhabditis elegans, stress-induced sleep(SIS) is regulated by cytokine activation of the ALA neuron, which releases FLP-13 neuropeptides characterized by an amidated arginine-phenylalanine (RFamide) C-terminus motif. By performing an unbiased genetic screen for mutants that impair the somnogenic effects of FLP-13 neuropeptides, we identified the gene dmsr-1, which encodes a G-protein coupled receptor similar to an insect RFamide receptor. DMSR-1 is activated by FLP-13 peptides in cell culture, is required for SIS in vivo, is expressed non-synaptically in several wake-promoting neurons, and likely couples to a Gi/o heterotrimeric G-protein. Our data expand our understanding of how a single neuroendocrine cell coordinates an organism-wide behavioral response, and suggest that similar signaling principles may function in other organisms to regulate sleep during sickness. KW - sleep KW - sickness KW - neuropeptides KW - GPCR KW - RFamide JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -