TY - JOUR TI - Ribosomal mutations promote the evolution of antibiotic resistance in a multidrug environment AU - Gomez, James E AU - Kaufmann-Malaga, Benjamin B AU - Wivagg, Carl N AU - Kim, Peter B AU - Silvis, Melanie R AU - Renedo, Nikolai AU - Ioerger, Thomas R AU - Ahmad, Rushdy AU - Livny, Jonathan AU - Fishbein, Skye AU - Sacchettini, James C AU - Carr, Steven A AU - Hung, Deborah T A2 - Gilmore, Michael S VL - 6 PY - 2017 DA - 2017/02/21 SP - e20420 C1 - eLife 2017;6:e20420 DO - 10.7554/eLife.20420 UR - https://doi.org/10.7554/eLife.20420 AB - Antibiotic resistance arising via chromosomal mutations is typically specific to a particular antibiotic or class of antibiotics. We have identified mutations in genes encoding ribosomal components in Mycobacterium smegmatis that confer resistance to several structurally and mechanistically unrelated classes of antibiotics and enhance survival following heat shock and membrane stress. These mutations affect ribosome assembly and cause large-scale transcriptomic and proteomic changes, including the downregulation of the catalase KatG, an activating enzyme required for isoniazid sensitivity, and upregulation of WhiB7, a transcription factor involved in innate antibiotic resistance. Importantly, while these ribosomal mutations have a fitness cost in antibiotic-free medium, in a multidrug environment they promote the evolution of high-level, target-based resistance. Further, suppressor mutations can then be easily acquired to restore wild-type growth. Thus, ribosomal mutations can serve as stepping-stones in an evolutionary path leading to the emergence of high-level, multidrug resistance. KW - Mycobacterium KW - antibiotic resistance KW - ribosome KW - fluoroquinolones JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -