TY - JOUR TI - A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem AU - Rabe, Franziska AU - Bosch, Jason AU - Stirnberg, Alexandra AU - Guse, Tilo AU - Bauer, Lisa AU - Seitner, Denise AU - Rabanal, Fernando A AU - Czedik-Eysenberg, Angelika AU - Uhse, Simon AU - Bindics, Janos AU - Genenncher, Bianca AU - Navarrete, Fernando AU - Kellner, Ronny AU - Ekker, Heinz AU - Kumlehn, Jochen AU - Vogel, John P AU - Gordon, Sean P AU - Marcel, Thierry C AU - Münsterkötter, Martin AU - Walter, Mathias C AU - Sieber, Christian MK AU - Mannhaupt, Gertrud AU - Güldener, Ulrich AU - Kahmann, Regine AU - Djamei, Armin A2 - Kliebenstein, Daniel J VL - 5 PY - 2016 DA - 2016/11/11 SP - e20522 C1 - eLife 2016;5:e20522 DO - 10.7554/eLife.20522 UR - https://doi.org/10.7554/eLife.20522 AB - Due to their economic relevance, the study of plant pathogen interactions is of importance. However, elucidating these interactions and their underlying molecular mechanisms remains challenging since both host and pathogen need to be fully genetically accessible organisms. Here we present milestones in the establishment of a new biotrophic model pathosystem: Ustilago bromivora and Brachypodium sp. We provide a complete toolset, including an annotated fungal genome and methods for genetic manipulation of the fungus and its host plant. This toolset will enable researchers to easily study biotrophic interactions at the molecular level on both the pathogen and the host side. Moreover, our research on the fungal life cycle revealed a mating type bias phenomenon. U. bromivora harbors a haplo-lethal allele that is linked to one mating type region. As a result, the identified mating type bias strongly promotes inbreeding, which we consider to be a potential speciation driver. KW - biotrophic interaction KW - effector KW - plant pathogen KW - grass KW - Ustilago bromivora KW - Brachypodium distachyon JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -