Identification of octopaminergic neurons that modulate sleep suppression by male sex drive
- Thomas Jefferson University, United States
- School of Medicine, University of Minho, Portugal
- ICVS/3B's - PT Government Associate Laboratory, Portugal
- Perelman School of Medicine at the University of Pennsylvania, United States
Figures
Figure 1 with 1 supplement
Balance between sleep drive and sex drive determines male sleep levels.
(A) Sleep profile in 30 min intervals for wild-type (iso31) flies in isolation (M for male, F for female) or in pairs (MM for male-male, MF for male-female) using multi-beam monitors. Corresponding …
Figure 1—figure supplement 1
Single-beam DAM data demonstrating reduced male sleep in the presence of females.
(A) Sleep profile in 30 min intervals for wild-type (iso31) flies in isolation (M, F) or in pairs (MM, MF). N = 45–52. (B) Daytime and nighttime sleep amount for flies shown in (A). Two-way ANOVA …
Figure 2 with 2 supplements
Sexual satiety and sleep deprivation attenuate female-induced sleep loss in males.
(A) Sleep profile of wild-type (iso31) MF pairs including satiated or non-satiated (naive) males assayed using single-beam monitors. N = 31–33. (B–C) Sleep amount (B) and courtship index (C) of …
Figure 2—figure supplement 1
Successful manipulation of sexual satiety in male flies.
(A) Percent of satiated males shown in Figure 2A exhibiting mating (courtship or copulation) behavior. Mating behavior was examined twice during the first hour (initial) and twice during the last …
Figure 2—figure supplement 2
Activation of dFSB induces sleep in isolated males.
Sleep profile of R23E10>NaChBac and parental control (R23E10/+ or NaChBac/+) males in isolation. N = 40–57. Daytime and nighttime sleep amounts were summed for one-way ANOVA followed by Dunnett post …
Figure 3
MS1 neuronal activity regulates sleep and mating behavior in males.
(A) Sleep profile of male (left) and female (right) flies expressing the warmth-activated TrpA1 channel in MS1 neurons (MS1>TrpA1) and control flies (MS1/+ and TrpA1/+). TrpA1 was activated by …
Figure 4
MS1 neurons in the SOG are octopaminergic.
(A) Confocal projection of a whole-mounted MS1> CD8::GFP male (left) or female (right) adult central brain. Antibodies against GFP (green) and Bruchpilot (BRP, magenta) were used for immunostaining. …
Figure 5 with 1 supplement
MS1 neurons appear to form synaptic contacts with FRU neurons.
(A) Confocal projection of a male brain expressing CD8::GFP in the intersection between MS1-Gal4 and fru-FLP (MS1-Gal4/UAS-FRT-stop-FRT-CD8::GFP; FRU-FLP/+). Scale bar: 100 µm. (B) Confocal …
Figure 5—figure supplement 1
MS1 neurons do not express FRU but likely form synaptic contacts with FRU neurons.
(A) Confocal projection of the lateral protocerebrum of a male brain expressing RFP (magenta) in MS1 neurons (MS1-Gal4 > UAS-CD8::RFP) and GFP (green) in FRU neurons (fru-LexA > LexAOp-CD8::GFP). …
Figure 6 with 1 supplement
Experimental activation of MS1 neurons elicits calcium responses in FRU neurons.
(A) GCaMP6m increase (△F) in FRU neurons of a dissected male brain in which MS1 neurons are activated by P2X2 expression and ATP perfusion. Antero-dorsal (left) and posterior (right) views are …
Figure 6—figure supplement 1
MS1 stimulation induces calcium responses in P1.
P2X2 was expressed under the control of MS1-Gal4, while GCaMP6m was expressed under the control of R71G01-LexA. A posterior view is presented using the ‘fire’ look-up table. Scale bar: 100 µm.
Figure 7
Activation of P1 neurons suppresses male sleep and experimental activation of FRU neurons elicits calcium responses in MS1 neurons.
(A) Sleep profile of males expressing NaChBac in MS1 neurons (MS1>NaChBac) and control males (MS1/+ and NaChBac/+) in an iso31 control (left), fruLexA/4-40 mutant (middle) and dsx683/1649 mutant …
Videos
Video 1
Wild-type male-male (MM) and male-female (MF) pairs at ~ZT18 under infrared light.
While most MM pairs slept, many males paired with females engaged in courtship behaviors including chasing and wing extension.
Video 2
Male flies with activated MS1 neurons (MS1 > TrpA1) and control males (MS1/+ or TrpA1/+) at 29°C.
The flies were recorded at ~ZT17 under infrared light. MS1 activation led to pacing behavior typically observed in awake flies (note one of the control flies exhibiting the same behavior).
Video 3
Male flies with activated P1 neurons and control males (left) as well as male flies with activated MS1 neurons and control males (right) at 29°C.
The flies were recorded ~30 min after the switch from 22°C to 29°C (ZT0.5) under white light. Wing extension is seen in males with activated P1 neurons (top left), but not in males with activated …
Video 4
Flies shown in Video 3 at ~ZT5 at 29°C.
Males with activated P1 neurons no longer exhibited wing extension, but engaged in behavior typical of awake flies such as pacing, feeding, and grooming.
