TY - JOUR TI - Planar cell polarity signaling coordinates oriented cell division and cell rearrangement in clonally expanding growth plate cartilage AU - Li, Yuwei AU - Li, Ang AU - Junge, Jason AU - Bronner, Marianne A2 - Robertson, Elizabeth VL - 6 PY - 2017 DA - 2017/10/10 SP - e23279 C1 - eLife 2017;6:e23279 DO - 10.7554/eLife.23279 UR - https://doi.org/10.7554/eLife.23279 AB - Both oriented cell divisions and cell rearrangements are critical for proper embryogenesis and organogenesis. However, little is known about how these two cellular events are integrated. Here we examine the linkage between these processes in chick limb cartilage. By combining retroviral-based multicolor clonal analysis with live imaging, the results show that single chondrocyte precursors can generate both single-column and multi-column clones through oriented division followed by cell rearrangements. Focusing on single column formation, we show that this stereotypical tissue architecture is established by a pivot-like process between sister cells. After mediolateral cell division, N-cadherin is enriched in the post-cleavage furrow; then one cell pivots around the other, resulting in stacking into a column. Perturbation analyses demonstrate that planar cell polarity signaling enables cells to pivot in the direction of limb elongation via this N-cadherin-mediated coupling. Our work provides new insights into the mechanisms generating appropriate tissue architecture of limb skeleton. KW - cell polarity KW - planar cell polarity signaling KW - cartilage development JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -