TY - JOUR TI - CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading AU - Pan, Dongqing AU - Klare, Kerstin AU - Petrovic, Arsen AU - Take, Annika AU - Walstein, Kai AU - Singh, Priyanka AU - Rondelet, Arnaud AU - Bird, Alexander W AU - Musacchio, Andrea A2 - Akhtar, Asifa VL - 6 PY - 2017 DA - 2017/01/06 SP - e23352 C1 - eLife 2017;6:e23352 DO - 10.7554/eLife.23352 UR - https://doi.org/10.7554/eLife.23352 AB - Centromeres are unique chromosomal loci that promote the assembly of kinetochores, macromolecular complexes that bind spindle microtubules during mitosis. In most organisms, centromeres lack defined genetic features. Rather, they are specified epigenetically by a centromere-specific histone H3 variant, CENP-A. The Mis18 complex, comprising the Mis18α:Mis18β subcomplex and M18BP1, is crucial for CENP-A homeostasis. It recruits the CENP-A-specific chaperone HJURP to centromeres and primes it for CENP-A loading. We report here that a specific arrangement of Yippee domains in a human Mis18α:Mis18β 4:2 hexamer binds two copies of M18BP1 through M18BP1’s 140 N-terminal residues. Phosphorylation by Cyclin-dependent kinase 1 (CDK1) at two conserved sites in this region destabilizes binding to Mis18α:Mis18β, limiting complex formation to the G1 phase of the cell cycle. Using an improved viral 2A peptide co-expression strategy, we demonstrate that CDK1 controls Mis18 complex recruitment to centromeres by regulating oligomerization of M18BP1 through the Mis18α:Mis18β scaffold. KW - kinetochore KW - centromere KW - cell cycle KW - CENP-A KW - chromatin JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -