TY - JOUR TI - Functional mapping of yeast genomes by saturated transposition AU - Michel, Agnès H AU - Hatakeyama, Riko AU - Kimmig, Philipp AU - Arter, Meret AU - Peter, Matthias AU - Matos, Joao AU - De Virgilio, Claudio AU - Kornmann, Benoît A2 - Deshaies, Raymond J VL - 6 PY - 2017 DA - 2017/05/08 SP - e23570 C1 - eLife 2017;6:e23570 DO - 10.7554/eLife.23570 UR - https://doi.org/10.7554/eLife.23570 AB - Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the Saccharomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows one-step mapping of all genetic loci in which transposons can insert without disrupting essential functions. SATAY is particularly suited to discover loci important for growth under various conditions. SATAY (1) reveals positive and negative genetic interactions in single and multiple mutant strains, (2) can identify drug targets, (3) detects not only essential genes, but also essential protein domains, (4) generates both null and other informative alleles. In a SATAY screen for rapamycin-resistant mutants, we identify Pib2 (PhosphoInositide-Binding 2) as a master regulator of TORC1. We describe two antagonistic TORC1-activating and -inhibiting activities located on opposite ends of Pib2. Thus, SATAY allows to easily explore the yeast genome at unprecedented resolution and throughput. KW - transposon KW - yeast KW - genetic interaction KW - pharmacogenomics KW - protein domains KW - TORC1 KW - Pib2 KW - Prp45 JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -