TY - JOUR TI - Regulation of plant immune receptor accumulation through translational repression by a glycine-tyrosine-phenylalanine (GYF) domain protein AU - Wu, Zhongshou AU - Huang, Shuai AU - Zhang, Xiaobo AU - Wu, Di AU - Xia, Shitou AU - Li, Xin A2 - Nürnberger, Thorsten VL - 6 PY - 2017 DA - 2017/03/31 SP - e23684 C1 - eLife 2017;6:e23684 DO - 10.7554/eLife.23684 UR - https://doi.org/10.7554/eLife.23684 AB - Plant immunity is tightly regulated to ensure proper defense against surrounding microbial pathogens without triggering autoimmunity, which negatively impacts plant growth and development. Immune receptor levels are intricately controlled by RNA processing and post-translational modification events, such as ubiquitination. It remains unknown whether, and if yes, how, plant immune receptor homeostasis is regulated at the translational level. From a mutant, snc1-enhancing (muse) forward genetic screen, we identified MUSE11/EXA1, which negatively regulates nucleotide-binding leucine-rich repeat (NLR) receptor mediated defence. EXA1 contains an evolutionarily conserved glycine-tyrosine-phenylalanine (GYF) domain that binds proline-rich sequences. Genetic and biochemical analysis revealed that loss of EXA1 leads to heightened NLR accumulation and enhanced resistance against virulent pathogens. EXA1 also associates with eIF4E initiation factors and the ribosome complex, likely contributing to the proper translation of target proteins. In summary, our study reveals a previously unknown mechanism of regulating NLR homeostasis through translational repression by a GYF protein. KW - plant immunity KW - resistance protein homeostasis KW - translational control KW - NLR receptor JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -