TY - JOUR TI - Insights into electrosensory organ development, physiology and evolution from a lateral line-enriched transcriptome AU - Modrell, Melinda S AU - Lyne, Mike AU - Carr, Adrian R AU - Zakon, Harold H AU - Buckley, David AU - Campbell, Alexander S AU - Davis, Marcus C AU - Micklem, Gos AU - Baker, Clare VH A2 - Petit, Christine VL - 6 PY - 2017 DA - 2017/03/27 SP - e24197 C1 - eLife 2017;6:e24197 DO - 10.7554/eLife.24197 UR - https://doi.org/10.7554/eLife.24197 AB - The anamniote lateral line system, comprising mechanosensory neuromasts and electrosensory ampullary organs, is a useful model for investigating the developmental and evolutionary diversification of different organs and cell types. Zebrafish neuromast development is increasingly well understood, but neither zebrafish nor Xenopus is electroreceptive and our molecular understanding of ampullary organ development is rudimentary. We have used RNA-seq to generate a lateral line-enriched gene-set from late-larval paddlefish (Polyodon spathula). Validation of a subset reveals expression in developing ampullary organs of transcription factor genes critical for hair cell development, and genes essential for glutamate release at hair cell ribbon synapses, suggesting close developmental, physiological and evolutionary links between non-teleost electroreceptors and hair cells. We identify an ampullary organ-specific proneural transcription factor, and candidates for the voltage-sensing L-type Cav channel and rectifying Kv channel predicted from skate (cartilaginous fish) ampullary organ electrophysiology. Overall, our results illuminate ampullary organ development, physiology and evolution. KW - Polyodon spathula (Mississippi paddlefish) KW - ampullary organs KW - electroreceptors KW - neuromasts KW - hair cells KW - synaptic ribbons KW - Atoh1 KW - Pou4f3 KW - Neurod4 KW - Vglut3 KW - otoferlin KW - Cav1.3 KW - Cavβ2 KW - beta-parvalbumins KW - oncomodulin KW - voltage-gated ion channels KW - Kv1.5 KW - Kvβ3 KW - hh JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -