Digitizing mass spectrometry data to explore the chemical diversity and distribution of marine cyanobacteria and algae

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Abstract

Natural product screening programs have uncovered molecules from diverse natural sources with various biological activities and unique structures. However, much is yet underexplored and additional information is hidden in these exceptional collections. We applied untargeted mass spectrometry approaches to capture the chemical space and dispersal patterns of metabolites from an in-house library of marine cyanobacterial and algal collections. Remarkably, 86% of the metabolomics signals detected were not found in other available datasets of similar nature, supporting the hypothesis that marine cyanobacteria and algae possess distinctive metabolomes. The data were plotted onto a world map representing 8 major sampling sites, and revealed potential geographic locations with high chemical diversity. We demonstrate the use of these inventories as a tool to explore the diversity and distribution of natural products. Finally, we utilized this tool to guide the isolation of a new cyclic lipopeptide, yuvalamide A, from a marine cyanobacterium.

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Tal Luzzatto Knaan

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, United States

For correspondence
tal.luzzatto@mail.huji.ac.il

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-8392-0501
Neha Garg

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Mingxun Wang

Center for Computational Mass Spectrometry, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Evgenia Glukhov

Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Yao Peng

Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Gail Ackermann

Department of Pediatrics, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Amnon Amir

Department of Pediatrics, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Brendan M Duggan

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Sergey Ryazanov

European Molecular Biology Laboratory, Heidelberg, Germany

Competing interests
The authors declare that no competing interests exist.
Lena Gerwick

Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Rob Knight

Department of Pediatrics, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Theodore Alexandrov

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Nuno Bandeira

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
William H Gerwick

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.
Pieter C Dorrestein

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, United States

Competing interests
The authors declare that no competing interests exist.

Funding

National Institutes of Health (GM107550)

William H Gerwick

European Union FP7

Theodore Alexandrov

H2020 (305259 and 634402)

Theodore Alexandrov

Vaadia-BARD Fellowship no.FI-494-13

Tal Luzzatto Knaan

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.