Replication Study: Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma
- ARQ Genetics, United States
Figures
Figure 1 with 2 supplements
Relative abundance of F. nucleatum in colorectal carcinoma versus adjacent normal biopsies in samples with detectable F. nucleatum.
Only samples in which F. nucleatum was detected in both colorectal carcinoma (CRC) and adjacent normal tissue by quantitative PCR (qPCR) and confirmed by sequencing of the qPCR product are shown (n =…
Figure 1—figure supplement 1
First independent qPCR run.
First of two independent qPCR runs performed in technical duplicate. (A) Distribution of raw Ct values for PGT in colorectal carcinoma (CRC) tumor, adjacent normal tissue, or matched normal samples. …
Figure 1—figure supplement 2
Second independent qPCR run.
Second of two independent qPCR runs performed in technical duplicate. (A) Distribution of raw Ct values for PGT in colorectal carcinoma (CRC) tumor, adjacent normal tissue, or matched normal …
Figure 2 with 1 supplement
Relative abundance of F. nucleatum by qPCR in colorectal carcinoma versus adjacent normal biopsies.
Fold change values are shown for all paired specimens based on differences in Ct values, irrespective of whether the qPCR products were confirmed to be specific or non-specific upon sequencing. …
Figure 2—figure supplement 1
Independent qPCR runs.
Fold change values are shown for all paired specimens based on differences in Ct values, irrespective of whether the qPCR products were confirmed to be specific or non-specific upon sequencing. (A) …
Figure 3
Meta-analyses of each effect.
Effect size (r) and 95% confidence interval are presented for Castellarin et al. (2012), this replication attempt (RP:CB), and a meta-analysis to combine the two effects. To directly compare and …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.25801.008
