Only samples in which F. nucleatum was detected in both colorectal carcinoma (CRC) and adjacent normal tissue by quantitative PCR (qPCR) and confirmed by sequencing of the qPCR product are shown (n = 6). The mean relative abundance of F. nucleatum (normalized to PGT expression) in CRC tissue versus adjacent normal tissue of both independent runs is reported for each patient sample and error bars represent SEM. The y-axis represents mean fold gene expression change (2-ΔΔCt) while the x-axis represents patient samples. Exploratory analysis: two-tailed paired t test: t(5) = 1.067, p = 0.335, r = 0.43, 95% CI [−0.59, 0.92]. Additional details for this experiment can be found at https://osf.io/rb4yq/.