TY - JOUR TI - Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development AU - van Lessen, Max AU - Shibata-Germanos, Shannon AU - van Impel, Andreas AU - Hawkins, Thomas A AU - Rihel, Jason AU - Schulte-Merker, Stefan A2 - Gerhardt, Holger VL - 6 PY - 2017 DA - 2017/05/12 SP - e25932 C1 - eLife 2017;6:e25932 DO - 10.7554/eLife.25932 UR - https://doi.org/10.7554/eLife.25932 AB - The lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and found loosely connected endothelial cells with lymphatic molecular signature covering parts of the brain without forming endothelial tubular structures. These brain lymphatic endothelial cells (BLECs) derive from venous endothelium, are distinct from macrophages, and are sensitive to loss of Vegfc. BLECs endocytose macromolecules in a selective manner, which can be blocked by injection of mannose receptor ligands. This first report on brain lymphatic endothelial cells in a vertebrate embryo identifies cells with unique features, including the uptake of macromolecules at a single cell level. Future studies will address whether this represents an uptake mechanism that is conserved in mammals and how these cells affect functions of the embryonic and adult brain. KW - lymphatics KW - endothelial cell KW - brain JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -