Ali-Murthy Z, Kornberg TB. 2016. Bicoid gradient formation and function in the Drosophila pre-syncytial blastoderm. eLife 5:e13222. doi: 10.7554/eLife.13222
Published 17, February 2016
In the published article the Fixation and de-vitellinization section of the Materials and methods, for Embryo in situ hybridization the wash buffer was listed as containing 1% Triton X-100. The wash buffer should have been described as containing 0.1% Triton X-100, and the relevant sentence should be: Embryos were de-chorionated in 50% bleach, washed with 0.1% Triton X-100 and transferred to a 20 ml vial containing fixative (5 ml heptane, 5 ml phosphate buffered saline (PBS), 50 mM EGTA (pH 8.0), 10.24% formaldehyde (Ultrapure), and shaken for 1.5 hour.
In the Pre-hybridization section of the Materials and methods, for Embryo in situ hybridization the pre-hybridization solution (HybS) was listed as containing 50% formaldehyde. HybS should have been described as containing 50% formamide. The correct sentence is: Embryos were incubated with rocking for: (1) 10 min at room temperature in 1 ml 1:1 hybridization solution (HybS):PBT (HybS; 50% formamide, 5xSSC, 100 μg/ml salmon sperm DNA, 50 μg/ml heparin, 0.1% Tween 20, 0.3% Triton X-100); (2) 10 min in 1 ml HybS at 55°C; (3) 45 min in 1 ml fresh HybS at 55°C; and (4) overnight in fresh HybS at 55°C.
The article has been corrected accordingly.
- Received: March 14, 2017
- Accepted: March 14, 2017
- Version of Record published: March 15, 2017 (version 1)
© 2017, Ali-Murthy et al.
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