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Correction: Bicoid gradient formation and function in the Drosophila pre-syncytial blastoderm

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Cite this article as: eLife 2017;6:e26811 doi: 10.7554/eLife.26811

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Ali-Murthy Z, Kornberg TB. 2016. Bicoid gradient formation and function in the Drosophila pre-syncytial blastoderm. eLife 5:e13222. doi: 10.7554/eLife.13222

Published 17, February 2016

In the published article the Fixation and de-vitellinization section of the Materials and methods, for Embryo in situ hybridization the wash buffer was listed as containing 1% Triton X-100. The wash buffer should have been described as containing 0.1% Triton X-100, and the relevant sentence should be: Embryos were de-chorionated in 50% bleach, washed with 0.1% Triton X-100 and transferred to a 20 ml vial containing fixative (5 ml heptane, 5 ml phosphate buffered saline (PBS), 50 mM EGTA (pH 8.0), 10.24% formaldehyde (Ultrapure), and shaken for 1.5 hour.

In the Pre-hybridization section of the Materials and methods, for Embryo in situ hybridization the pre-hybridization solution (HybS) was listed as containing 50% formaldehyde. HybS should have been described as containing 50% formamide. The correct sentence is: Embryos were incubated with rocking for: (1) 10 min at room temperature in 1 ml 1:1 hybridization solution (HybS):PBT (HybS; 50% formamide, 5xSSC, 100 μg/ml salmon sperm DNA, 50 μg/ml heparin, 0.1% Tween 20, 0.3% Triton X-100); (2) 10 min in 1 ml HybS at 55°C; (3) 45 min in 1 ml fresh HybS at 55°C; and (4) overnight in fresh HybS at 55°C.

The article has been corrected accordingly.

Article and author information

Author details

  1. Zehra Ali-Murthy

Publication history

  1. Received: March 14, 2017
  2. Accepted: March 14, 2017
  3. Version of Record published: March 15, 2017 (version 1)

Copyright

© 2017, Ali-Murthy et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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