(A) A schematic diagram of a stage 9 Drosophila egg chamber showing oskar mRNA transport. (B–C) Confocal images of stage 9 egg chambers from a wild-type ovary (B) and from a 4D2 homozygous germline …
(A–D) Confocal images of stage 9 (A and B) and stage 10b egg chambers (C and D) from wild-type (A and C) and 4D2 germline clone ovaries (B and D) stained for Staufen (A–D) and Oskar (A’–D’) …
(A–B) Wild-type (A) and arp14D2 mutant (B) stage 9 egg chambers stained for kinesin-βgal. (C–D) Wild-type (C–C’’) and arp14D2 (D–D’’) mutant stage 9 egg chambers stained for p150Glued (C–D) and the …
(A) Diagram of the right arm of chromosome 3 showing the visible markers used for recombination mapping (cu sr e ca) and the position of the Arp1 locus. (B) Diagram showing the extent of the …
(A–D) Confocal images of GFP-Staufen (green) and DNA (DAPI; blue) in arp14D2/+ (A), an arp14D2 germ line clone (B), arp14D2/ arp11 (C) and arp14D2/ arp12 (D) stage 9 egg chambers. Scale bar 20 μm.
(A) A ClustalX alignment of part of subdomain 2 (amino acids 26–78) in the Arp1 orthologues from different animal species and Drosophila Actin 5C. The orthologues shown are Homo sapiens (human), Ratt…
(A–A”) A confocal image of a germarium from an arp14D2/+heterozygote stained for the oocyte marker Orb (white in A; red in A’) and expressing nls-GFP (white in A’; green in A’). (B–B”) A confocal …
(A) A graph showing the relative frequency of oskar mRNA movements at different distances from the posterior pole in arp14D2/+ (n = 528 tracks) and arp14D2 mutant oocytes (n = 410). The posterior …
The number of oskar mRNA tracks at the specified distances from the posterior pole.
Direction of oskar mRNA tracks at the specified distances from the posterior pole.
(A) A histogram showing the average velocities (µm/sec) of EB1 comets on growing microtubule plus ends in the centre of the oocyte (15–30 µm from the posterior) and near the posterior cortex (0–15 …
Velocities of EB1-GFP comets at the specified distances from the posterior pole.
Distances of EB1-GFP tracks from the posterior cortex.
Lifespan of EB1-GFP comets near the posterior cortex.
(A) A histogram showing the average velocities (µm/sec) of EB1 comets on growing microtubule plus ends in the centre of the oocyte (15–30 µm from the posterior) and near the posterior cortex (0–15 µm…
(A) A confocal image of microtubules in a wild-type stage 9 oocyte indicating the regions of the posterior and lateral cortex (yellow lines) from which the kymographs in (C) were collected. (B) A …
Dwell time of EB1-GFP comets at the lateral and posterior cortex
A series of diagrams showing the steps in the positive feedback loop that ensures that microtubules reach the posterior cortex to deliver oskar mRNA. The distribution of cortical ncMTOCs along the …
Phenotype | Average speed, μm/s | Tracks to Posterior, % | Mobile fraction, % |
---|---|---|---|
WT | 0.36 ± 0.01 (N=1075) | 65 ± 0.8 (N=989) | 12.0 ± 2.1 (N = 358) |
arp14D2 | 0.37 ± 0.03 (N=296) | 62 ± 0.7 (N=353) | 15.4 ± 2.5 (N = 361) |
Values shown are means plus and minus the SEM. In all cases, the differences between wild-type and the arp1[4D2] mutant were statistically insignificant, p>0.05.
Speed of oskar mRNA particles.
Direction of oskar mRNA particles.
Mobile fraction of oskar mRNA particles.